Showing: 41 - 60 of 12549 results
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Plasmid#47932Purposein vitro transcription of ddx19 gRNADepositorInsertddx19 gRNA (ddx19 Zebrafish)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pIK86
Plasmid#47933PurposeC. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.DepositorInsertCas9
UseCRISPR and UnspecifiedTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMB60
Plasmid#47941PurposeCan be used to generate synthetic guide in vitro from T7 promotorDepositorInsertsynthetic guide RNA
UseCRISPRTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMB70
Plasmid#47943PurposeCan be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequencesDepositorInsertguide RNA
UseCRISPRTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMB62
Plasmid#47944PurposeExpresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormMutationPromotereef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
pMB63
Plasmid#47945PurposeExpresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, and egl-13 NLSExpressionWormMutationPromotereef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
pMB66
Plasmid#47946PurposeExpresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormMutationPromoterhsp-16.48AvailabilityAcademic Institutions and Nonprofits only -
pMB67
Plasmid#47947PurposeExpresses C. elegans optimized Cas9 from the hsp-16.48 promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, and egl-13 NLSExpressionWormMutationPromoterhsp-16.48AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pCMV-7.1
Plasmid#47948PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840A (catalyticall…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (PX165)
Plasmid#48137PurposeHuman codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)DepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianMutationPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP (PX458)
Plasmid#48138PurposeCas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorHas ServiceCloning Grade DNAInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-GFP (PX461)
Plasmid#48140PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorInserthSpCas9n
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462)
Plasmid#48141PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62987DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pHsp70-Cas9
Plasmid#45945PurposeThe codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.DepositorInsertCodon optimized Cas9
UseCRISPRTags3x FLAGExpressionMutationSilent change from C to A at bp 834 of insertPromoterAvailabilityAcademic Institutions and Nonprofits only -
pU6-BbsI-chiRNA
Plasmid#45946PurposePlasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.DepositorInsertU6-BbsI-chiRNA
UseCRISPRTagsExpressionInsectMutationPromoterDm-snRNA:U6:96AbAvailabilityAcademic Institutions and Nonprofits only -
MLM3636
Plasmid#43860Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoterDepositorInsertNone
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
JDS246
Plasmid#43861PurposeExpresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promotersDepositorInsertmammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
UseCRISPRTags3X FLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
p3s-Cas9HC
Plasmid#43945DepositorInsertCas9
UseCRISPRTagsHA epitope and NLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pdCas9-humanized
Plasmid#44246PurposeExpression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdownDepositorInsertdead Cas9 with 3X NLS
UseCRISPR and Retroviral; VectorTags3x NLSExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterMSCV LTR promoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 41 - 60 of 12549 results