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Addgene
Showing: 61 - 80 of 170 results

  5. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid
    #194328
    Purpose
    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness
    Depositor
    Alexander McLellan
    Article
    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Tet-responsive promotor related mutations: G72V i…
    Promoter
    Tight tet-responsive promotor
    Availability
    Academic Institutions and Nonprofits only
    194328-plasmid-map-sequence-id-383152

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  12. pSB-Bleo STAT3

    Plasmid
    #139184
    Purpose
    Empty backbone encoding constitutive bleomycin resistance with an inducible STAT3 promoter to allow for an insert in a Sleeping Beauty backbone.
    Depositor
    Neal Devaraj
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    STAT3
    Availability
    Academic Institutions and Nonprofits only
    139184-plasmid-map-sequence-id-309370

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  15. pT2/shαSMA/GFP4_Seq2/clone1

    Plasmid
    #201402
    Purpose
    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    alpha-SMA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    201402-plasmid-map-sequence-id-394260

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  16. pSB-Bleo Gal4 tBFP

    Plasmid
    #139190
    Purpose
    Gal4-inducible tag blue fluorescent protein (tBFP) in pSB-Bleo Gal4 Sleeping Beauty backbone.
    Depositor
    Neal Devaraj
    Insert
    tBFP
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Gal4 CMV and STAT3
    Availability
    Academic Institutions and Nonprofits only
    139190-plasmid-map-sequence-id-309397

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  17. pT2/SV-Venus-SIINFEKL

    Plasmid
    #111624
    Purpose
    Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies
    Depositor
    Jon Yewdell
    Insert
    Venus-LEQLE-SIINFEKL-TEW
    Use
    Tags
    LEQLE-SIINFEKL-TEW
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Availability
    Academic Institutions and Nonprofits only
    111624-plasmid-map-sequence-id-213225

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  18. pSBBi-Pur LaG17-synNotch-TetRVP64

    Plasmid
    #158133
    Purpose
    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
    Depositor
    Neal Devaraj
    Insert
    LaG17-synNotch-TetRVP64
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    158133-plasmid-map-sequence-id-309383

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  19. pSBBi-Pur LaG17-synNotch-Gal4VP64

    Plasmid
    #158135
    Purpose
    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
    Depositor
    Neal Devaraj
    Insert
    LaG17-synNotch-Gal4VP64
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    158135-plasmid-map-sequence-id-309380

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  20. pSBbi-RP Hu CXCR3

    Plasmid
    #183255
    Purpose
    Expression of human CXCR3 in a Sleeping Beauty transposon vector
    Depositor
    Alexander McLellan
    Insert
    CXCR3 (CXCR3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    183255-plasmid-map-sequence-id-369698

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Showing: 61 - 80 of 170 results