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Showing: 21 - 40 of 62 results

  2. pFloxed DHFR-TS*

    Plasmid
    #100605
    Purpose
    pUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTR
    Depositor
    David Sibley
    Insert
    Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    100605-plasmid-map-sequence-id-192861

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  3. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Expression
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Availability
    Academic Institutions and Nonprofits only
    87258-plasmid-map-sequence-id-176556

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  4. pUPRT::Floxed DHFR-TS*

    Plasmid
    #100606
    Purpose
    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.
    Depositor
    David Sibley
    Insert
    TgUPRT::Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    pTgDHFR-TS
    Availability
    Academic Institutions and Nonprofits only
    100606-plasmid-map-sequence-id-192862

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  8. pCAT-YFP-TetR

    Plasmid
    #59018
    Purpose
    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Insert
    YFP-TetR
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    alpha-tubulin
    Availability
    Academic Institutions and Nonprofits only
    59018-plasmid-map-sequence-id-137741

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  13. ATSR

    Plasmid
    #50052
    Purpose
    expresses t.gondii VWA+TSR1 domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-ATSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A
    Promoter
    Chick beta-actin promoter
    Availability
    Academic Institutions and Nonprofits only

  15. proATSR

    Plasmid
    #50053
    Purpose
    Expresses T.gondii prodomain+VWA+TSR1 domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-proATSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A
    Promoter
    chick beta-actin promoter
    Availability
    Academic Institutions and Nonprofits only

  16. A5TSR

    Plasmid
    #50358
    Purpose
    expresses T.gondii MIC2(67-593) domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-A5TSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CHICK beta-actin promoter
    Availability
    Academic Institutions and Nonprofits only

  17. A6TSR

    Plasmid
    #50359
    Purpose
    expresses T.gondii MIC2(67-660) domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-A6TSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CHICK beta-actin promoter
    Availability
    Academic Institutions and Nonprofits only

  18. PP-mACP

    Plasmid
    #25124
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    59.m03510:C40207
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  19. PP-ROP2A

    Plasmid
    #25130
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    33.m01398:C43601
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  20. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid
    #86668
    Purpose
    for generation of BirA tagged strains using CRISPR tagging technology
    Depositor
    David Sibley
    Insert
    Linker-BirA-3xHA-HXGPRT 3'UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    86668-plasmid-map-sequence-id-190141

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Showing: 21 - 40 of 62 results