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Plasmid#25123PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert33.m00005:C33888 (TGME49_039600 Toxoplasma gondii)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-CyP8
Plasmid#25122PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert541.m00136:C37419
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
Tg-UBC (TgTwinScan_2218)
Plasmid#25565PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTg-UBC (TgTwinScan_2218)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-CyP5
Plasmid#25121PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert49.m03261:C37453
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUPRT::DHFR-D
Plasmid#58528Purposereplace TgUPRT with DHFR by homologous recombinationDepositorInsertDHFR cassette
UseGateway cloningTagsExpressionMutationpyrimethamine resistantPromoterDHFRAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
PP-CDPK4 (3MA6)
Plasmid#25275PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTgCDPK4:P36-Q315
UseTagsHisExpressionBacterialMutationContains amino acids P36-Q315PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-PYRK1 (3GG8)
Plasmid#25288PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert55.m00007:I39-E531
UseTagsHisExpressionBacterialMutationContains amino acids I39-E531PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-ACP
Plasmid#25188PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert55.m00019:s:S100-S180
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-CDPK1 (3HZT)
Plasmid#25189PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert541.m00134:L72-H537
UseTagsHisExpressionBacterialMutationContains amino acids L72-H537PromoterAvailable sinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-CDPK6 (3IS5)
Plasmid#25194PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert38.m00014:T112-A378
UseTagsHisExpressionBacterialMutationContains amino acids T112-A378PromoterAvailable sinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-CDPK1 (3DXN)
Plasmid#25129PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert541.m00134:MAC02T-C05:C40091
UseTagsHisExpressionBacterialMutationContains amino acids L72-Q339PromoterAvailable sinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-MAPK2 (3RP9)
Plasmid#32862PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK2
UseTagsHis tag with TEV cleavageExpressionBacterialMutationamino acids 123-548 only, P421LPromoterAvailable sinceJan. 10, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGCaMP5
Plasmid#78705PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable markerDepositorInsertGCaMP5
UseTagsExpressionMutationPromoterSAG1 5' UTRAvailable sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
3HX4
Plasmid#60137PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTgCDPK4:MAC03F-A01:C208088
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-UPRT
Plasmid#69310Purposeexpresses UPRT cDNADepositorInsertUPRT cDNA
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-HA::UPRT
Plasmid#47110Purposefor subcloning UPRT into expression vectorsDepositorInserturacil phosphoribosyltransferase
UseTagsHAExpressionMutationPromoterAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTet-On-TAP
Plasmid#59017PurposeConstruct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.DepositorTypeEmpty backboneUseToxoplasma expressionTagsCalmodulin Binding Peptide fused with a TEV Cleav…ExpressionMutationPromoterRPS13 with Tet Operators InsertedAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-UPRT-T2A-RFP-IRES-CD
Plasmid#126677PurposeDoxycycline inducible expression of UPRT and CD along with reporter RFPDepositorInsertUPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tagger
Plasmid#129396PurposeExpresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoterDepositorInsertrpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
UseTagsFLAG, HA, and V5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiCre-mCherry
Plasmid#164572PurposeIntegration of a DiCre cassette in PbGFP parasite genomeDepositorInsertsFKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
UseCre/LoxTagsExpressionMutationPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only