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Addgene
Showing: 41 - 60 of 333 results

  5. p1A CbbL-

    Plasmid
    #162693
    Purpose
    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Use
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Mutation
    CbbL K194M (inactive rubisco)
    Promoter
    PLtet0-1 promoter
    Availability
    Academic Institutions and Nonprofits only
    162693-plasmid-map-sequence-id-324169

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  8. pCB’

    Plasmid
    #162703
    Purpose
    pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    pHnCB10 derived carboxysome operon, prk
    Use
    Tags
    Expression
    Bacterial
    Mutation
    5513T>G (tetR E37A); 7758G>T (second tet op…
    Promoter
    PLtet0-1 promoter
    Availability
    Academic Institutions and Nonprofits only
    162703-plasmid-map-sequence-id-324199

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  9. pCCM

    Plasmid
    #162708
    Purpose
    Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLtet0-1 promoter
    Availability
    Academic Institutions and Nonprofits only
    162708-plasmid-map-sequence-id-324195

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  10. pCCM’

    Plasmid
    #162709
    Purpose
    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    p15A origin replaced with high-copy colE1 origin
    Promoter
    PLtet0-1 promoter
    Availability
    Academic Institutions and Nonprofits only
    162709-plasmid-map-sequence-id-341131

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  11. pCB’ CsoS2 ΔNTD

    Plasmid
    #162705
    Purpose
    Carboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    pHnCB10 derived carboxysome operon, prk
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Deletion of residues 2-256 of CsoS2, Y131D
    Promoter
    PLtet0-1 promoter
    Availability
    Academic Institutions and Nonprofits only
    162705-plasmid-map-sequence-id-341116

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  12. pCB’ CbbL Y72R

    Plasmid
    #162707
    Purpose
    Carboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomes
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    pHnCB10 derived carboxysome operon, prk
    Use
    Tags
    Expression
    Bacterial
    Mutation
    CbbL Y72R; Substitution of CsoS2-binding tyrosine…
    Promoter
    PLtet0-1 promoter
    Availability
    Academic Institutions and Nonprofits only
    162707-plasmid-map-sequence-id-326996

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  15. p1A Prk-

    Plasmid
    #162694
    Purpose
    p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Use
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Mutation
    Prk inactive (K20M S21A native protein, this map …
    Promoter
    PLtet0-1 promoter
    Availability
    Academic Institutions and Nonprofits only
    162694-plasmid-map-sequence-id-324149

    Enlarge

  16. pcDNA3.1-3'3xFlag

    Plasmid
    #208616
    Purpose
    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    208616-plasmid-map-sequence-id-418312

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  17. pcDNA3.1-3'TwStrep

    Plasmid
    #208618
    Purpose
    Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    208618-plasmid-map-sequence-id-418314

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  18. pET28-5'TwStrep

    Plasmid
    #208620
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    208620-plasmid-map-sequence-id-415919

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Showing: 41 - 60 of 333 results