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Showing: 1 - 20 of 36 results

  2. pGCP123-EbpA_g1

    Plasmid
    #153517
    Purpose
    sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded
    Depositor
    Kimberly Kline
    Article
    Afonina et al mBio. 2020
    Insert
    pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    153517-plasmid-map-sequence-id-307072

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  3. pGCP123-GFP_g1

    Plasmid
    #153518
    Purpose
    sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded
    Depositor
    Kimberly Kline
    Article
    Afonina et al mBio. 2020
    Insert
    PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    153518-plasmid-map-sequence-id-307069

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  4. pRecT_2

    Plasmid
    #167546
    Purpose
    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Insert
    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    IPTG
    Availability
    Academic Institutions and Nonprofits only
    167546-plasmid-map-sequence-id-332204

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  5. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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  7. pSMART-Mev1

    Plasmid
    #65815
    Purpose
    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    insulated mipAp from E. coli and insulated waaHp…
    Availability
    Academic Institutions and Nonprofits only
    65815-plasmid-map-sequence-id-111947

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Showing: 1 - 20 of 36 results