-
Plasmid#209085PurposeNgn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only
-
piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry
Plasmid#182560PurposeExpresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherryDepositorInsertsmouse Scn8a ICL1
sfGFP-P2A-mCherry
UseTagsExpressionMammalianMutationPromoterSynAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)
Plasmid#209086PurposeNgn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsAviTagExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)
Plasmid#209079PurposeNgn2 mediated cortical neuron induction, along with sfGFP over-expressionDepositorInsertsUseTagsExpressionMammalianMutationPromoterTRE4GAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)
Plasmid#209082PurposeNFIB and SOX9 mediated astrocye inductionDepositorUseTagsExpressionMammalianMutationPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4
Plasmid#217971PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.DepositorInsert2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)
Plasmid#209077PurposeNgn2 mediated cortical neuron induction, dox induction is includedDepositorInsertsUseTagsExpressionMammalianMutationPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)
Plasmid#209078PurposeNgn2 mediated cortical neuron induction, along with SNCA(3K) over-expressionDepositorUseTagssfGFPExpressionMammalianMutation3K mutant: E35K, E46K, E61KPromoterTRE4GAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)
Plasmid#209080PurposeNgn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expressionDepositorUseTagssfGFPExpressionMammalianMutationA53TPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)
Plasmid#209081PurposeNgn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expressionDepositorUseTagssfGFPExpressionMammalianMutationA53T, NAC deletion (aa60-95)PromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-PB
Plasmid#133885PurposepiggyBac ITR's in high copy number pBluescript backbone plasmidDepositorTypeEmpty backboneUsePiggybacTagsExpressionMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p15A-PB
Plasmid#133886PurposepiggyBac ITR's in low copy number p15A backbone plasmidDepositorTypeEmpty backboneUsePiggybacTagsExpressionMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-minimal PB
Plasmid#177151PurposeMinimal piggyBac ITR's in high copy number pBluescript backbone plasmidDepositorTypeEmpty backboneUsePiggybacTagsExpressionMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-MCS-Puro
Plasmid#219794PurposeMammalian expression plasmids using piggyBac Transposon SystemDepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CA retrofitted
Plasmid#159093PurposepiggyBac ITR's from PB-CA retrofitted with a linkerDepositorTypeEmpty backboneUsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-MCS-Puro-EGFP
Plasmid#219795PurposeMammalian expression plasmids using piggyBac Transposon SystemDepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CA sfGFP
Plasmid#159094PurposePB-CA sfGFP piggyBac transposonDepositorInsertsfGFP
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CA tdTomato
Plasmid#159095PurposePB-CA tdTomato piggyBac transposonDepositorInserttdTomato
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-iCre-ERT2
Plasmid#137858PurposeCAG-iCre-ERT2 piggyBac transposonDepositorInsertiCre-ERT2
UseCre/Lox; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-mTagBFP2-T2A-iCreERT2
Plasmid#177308PurposeCAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposonDepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/Lox; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only