Showing: 41 - 60 of 65 results
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Plasmid#40253DepositorInsertLOV-ipaA L514K L531E
UseTags6xHisExpressionBacterialMutationL514K; L531E; Jα helix region hybridized (AsLOV2 …PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
EGFP-IpgD
Plasmid#183666PurposeN-terminally tagged Shigella flexneri IpgD for mammalian expressionDepositorInsertIpgD
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-IpgD
Plasmid#183672PurposeInducible Shigella flexneri IpgD for expression in yeastDepositorInsertIpgD
UseTagsExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pRMCD28
Plasmid#167556PurposePlasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneUseTagsPhoA'ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRMCD70
Plasmid#167557PurposePlasmid pWSK29 encoding E. coli LacZ' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneUseTagsLacZ'ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
EGFP-IpgD(C439S)
Plasmid#183667PurposeN-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expressionDepositorInsertIpgD
UseTagsEGFPExpressionMammalianMutationC439SPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-IpgD(C439S)
Plasmid#183673PurposeInducible Shigella flexneri IpgD (catalytically inactive) for expression in yeastDepositorInsertIpgD
UseTagsExpressionYeastMutationC439SPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pCACTUS
Plasmid#167559PurposeSuicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.DepositorTypeEmpty backboneUseSuicide cloning vector, temperature sensitive.TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pnCas9-AID
Plasmid#196242PurposePlasmid expressing the nCas9-AID fusionDepositorInsertnickase Cas9 fused to AID
UseCRISPRTagsExpressionBacterialMutationPromotertac promoterAvailabilityAcademic Institutions and Nonprofits only -
pGL0_34 [PaadA]
Plasmid#198947PurposeLevel 0 partDepositorInsertPaadA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGL0_35 [aadA]
Plasmid#198948PurposeLevel 0 partDepositorInsertaadA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRMCD30
Plasmid#173033PurposepQE30 with S. flexneri 2a wzzBSF geneDepositorInsertS. flexneri 2a wzzBSF gene
UseTags6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGL0_7 [pSa ORI]
Plasmid#198923PurposeLevel 0 partDepositorInsertpSa ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FLAG-NLRP10
Plasmid#38141DepositorInsertNLRP10 (NLRP10 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLG005 (Retron-TIR)
Plasmid#157883PurposeExpresses the retron-TIR defense systemDepositorInsertRetron-TIR
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
4A3.SU
Plasmid#197675PurposeExpresses P1 S tail fibre and U chaperoneDepositorInsertsS
U
UseTagsExpressionBacterialMutationPromoterLate S promoter, LPS and N/AAvailabilityAcademic Institutions and Nonprofits only -
4A3.S'U'
Plasmid#197676PurposeExpresses P1 S' tail fibre and U' chaperoneDepositorInsertsS'
U'
UseTagsExpressionBacterialMutationPromoterLate S promoter, LPS and N/AAvailabilityAcademic Institutions and Nonprofits only -
pBM019
Plasmid#128179PurposeConstruct for generating stable Cas9-expressing Toxoplasma gondiiDepositorInsertssgRNA#1
SpCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalExpressionMutationPromoterTUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…AvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)
Plasmid#171089PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsDecoy sgRNA
dCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalExpressionMutationD10A, H840APromoterTUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…AvailabilityAcademic Institutions and Nonprofits only -
pBBR1.cas9-NT
Plasmid#197871PurposeConstitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)DepositorInsertstrancCRNA
Cas9
crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 41 - 60 of 65 results