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Showing: 61 - 80 of 174 results

  2. pHJ17

    Plasmid
    #48356
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48356-plasmid-map-sequence-id-70042

    Enlarge

  3. pHJ18

    Plasmid
    #48357
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48357-plasmid-map-sequence-id-70046

    Enlarge

  4. pHJ22

    Plasmid
    #48358
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Neomycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-NEO-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48358-plasmid-map-sequence-id-70047

    Enlarge

  5. pHJ42

    Plasmid
    #48359
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Bleomycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BLE-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48359-plasmid-map-sequence-id-70049

    Enlarge

  6. pHJ41

    Plasmid
    #48360
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    48360-plasmid-map-sequence-id-70051

    Enlarge

  11. p221- purVSG117UTR

    Plasmid
    #59732
    Purpose
    Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.
    Depositor
    Gloria Rudenko
    Article
    Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:
    Insert
    VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
    Use
    For homologous recombination into vsg221 expressi…
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    59732-plasmid-map-sequence-id-100249

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  12. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

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  13. pLEW100v5-trunc

    Plasmid
    #33346
    Depositor
    George Cross
    Insert
    Luciferase truncated to allow cytoplasmic instead of peroxisomal (glycosomal) expression
    Use
    Trypanosoma brucei expression vector
    Tags
    Expression
    Mutation
    21bp of wt luciferase deleted
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    33346-plasmid-map-sequence-id-39752

    Enlarge

  14. pRP-iSL(MCS1+2)

    Plasmid
    #69245
    Purpose
    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a random RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    Expression
    Mutation
    Promoter
    RRNA promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 61 - 80 of 174 results