Addgene

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TUBG1

Plasmid

#87954

Purpose

expresses human gamma-tubulin with a GFP-tag on the N-terminal of the protein

Depositor

Maria Alvarado-Kristensson

Article

Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

whole protein, The C-terminal is a sh-gamma-tubul…

Promoter

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TUBG1

Plasmid

#171967

Purpose

pGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion protein

Depositor

Maria Alvarado-Kristensson

Article

Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

Insert

TUBG1

Use

Tags

GST

Expression

Bacterial

Mutation

R275H (Please see depositor comments)

Promoter

Availability

Academic Institutions and Nonprofits only

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TUBG1_pLX307

Plasmid

#98376

Purpose

Lentiviral expression of TUBG1

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TUBG1 (TUBG1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Availability

Academic Institutions and Nonprofits only

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TUBG1 F7.4 gRNA

Plasmid

#90930

Purpose

3rd generation lentiviral gRNA plasmid targeting human TUBG1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TUBG1 (Guide Designation F7.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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TUBG1 F8.4 gRNA

Plasmid

#90931

Purpose

3rd generation lentiviral gRNA plasmid targeting human TUBG1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TUBG1 (Guide Designation F8.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pBactin-Tubg1-mNeonGreen

Plasmid

#196869

Purpose

Expression of the gamma-tubulin fused to mNeonGreen

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Tubg1-mNeonGreen (Tubg1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Availability

Academic Institutions and Nonprofits only

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pBactin-Tubg1-mEGFP

Plasmid

#196870

Purpose

Expression of the gamma-tubulin fused to enhanced (E)-GFP

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Tubg1-mEGFP (Tubg1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-gamma-tubulin

Plasmid

#87956

Purpose

Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein

Depositor

Ana Carrera, Maria Alvarado-Kristensson

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

The protein is a sh-gamma-tubulin resistant gene …

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
gamma-tubulin (334-451)

Plasmid

#104436

Purpose

expressing a fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin fragment 334 to 451 (TUBG1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

deleted a.a. 1 to 333

Promoter

Availability

Academic Institutions and Nonprofits only

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pET21 gamma-tubulin

Plasmid

#101825

Purpose

production of gamma-tubulin in bacteria

Depositor

Ana Carrera, Maria Alvarado-Kristensson

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Whole protein

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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sg resistant gamma-tubulin

Plasmid

#104433

Purpose

For expressing gamma-tubulin in cells expressing γTUBULIN single guide RNA

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

None

Expression

Mammalian

Mutation

changed t bp28 to c; g bp30 to a; c bp33 to a; …

Promoter

Availability

Academic Institutions and Nonprofits only

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pACEBac1-gamma-tubulin-TEV-His6

Plasmid

#178067

Purpose

Insect cell expression of gamma-tubulin-TEV-His6

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

gamma-tubulin (TUBG1 Human)

Use

Tags

TEV-His6

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MAC_N_TBG1

Plasmid

#111696

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TBG1 (TUBG1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pACEBac1-gamma-tubulin(N229A)-TEV-His6

Plasmid

#178077

Purpose

Insect cell expression of gamma-tubulin(N229A)-TEV-His6

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

gamma-tubulin with an N229A point mutation (TUBG1 Human)

Use

Tags

TEV-His6

Expression

Insect

Mutation

Changed Asparagine 229 to Alanine

Promoter

Availability

Academic Institutions and Nonprofits only

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pTER GAMMA-TUBULIN shRNA Human

Plasmid

#87955

Purpose

reduced the expression of gamma-tubulin in human cells

Depositor

Ana Carrera, Maria Alvarado-Kristensson

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

sh gamma-tubulin (TUBG1 Human)

Use

Tags

No-tag

Expression

Mammalian

Mutation

The protein is a sh-gamma-tubulin resistant gene.…

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-N-gamma-tubulin (1-333)

Plasmid

#87858

Purpose

Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Fragment from aa 1 to 333

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPN1-N-gamma-tubulin (334-449)

Plasmid

#87859

Purpose

Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

fragment from aa 334 to 449

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS/U6/RNAi gamma-tubulin shRNA mouse

Plasmid

#70666

Purpose

Reduced the expression of gamma-tubulin in murine cells

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)

Use

U6 promoter

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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mEmerald-Gamma-Tubulin

Plasmid

#54105

Purpose

Localization: Centrosomes, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Gamma-Tubulin (TUBG1 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

R47H

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EBFP2-Gamma-Tubulin-17

Plasmid

#55242

Purpose

Localization: Centrosomes, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

Gamma-Tubulin (TUBG1 Human)

Use

Tags

EBFP2

Expression

Mammalian

Mutation

R57H in Gamma Tubulin

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits