We narrowed to 102 results for: cas12a

1. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

   Plasmid

   #107942

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
   

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R, and N282A

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

   Plasmid

   #107943

   Purpose

   Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
   

   Tags

   3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

   Plasmid

   #114073

   Purpose

   T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
   

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   E174R, S542R, K548R and N282A

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJC005.em

   Plasmid

   #182738

   Purpose

   CRISPR-Cas12a genome editing of E. faecium

   Depositor

   James Collins

   Article

   Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

   Plasmid

   #140220

   Purpose

   Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   dLbCpf1
   

   Use

   CRISPR

   Tags

   miniVPR-mCherry

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   PGK

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Promoter

   EF1a

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

   Plasmid

   #140086

   Purpose

   Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   LbCpf1 (LbCas12a) crRNA backbone
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-BFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUCsRNAP

   Plasmid

   #182746

   Purpose

   Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids

   Depositor

   James Collins

   Article

   Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

   Insert

   small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
   

   Use

   CRISPR

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBE48

   Plasmid

   #174656

   Purpose

   CAPTURE method BAC origin of replication receiver plasmid

   Depositor

   Huimin Zhao

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF525-EF1a-Hygro-P2A-mCherry-lenti

    Plasmid

    #115796

    Purpose

    Expresses mCherry in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EF-1a promoter

    Available Since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBE45

    Plasmid

    #174655

    Purpose

    CAPTURE method receiver plasmid for Streptomyces heterologous expression

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBE44

    Plasmid

    #174654

    Purpose

    CAPTURE method 15A origin of replication receiver plasmid

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR-Hyg

    Plasmid

    #158708

    Purpose

    For cloning and constitutive expression of crRNAs in mycobacterium

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    crRNA
    

    Use

    CRISPR and Synthetic Biology; Mycobacteria expres…

    Expression

    Bacterial

    Promoter

    Hsp60

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115268

    Purpose

    Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    EiCsm6
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMBP-AaCas12b

    Plasmid

    #113433

    Purpose

    Expresses Expresses 10xHis-MBP fused to AaCas12b

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AaCas12b
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBE14

    Plasmid

    #174653

    Purpose

    In vivo Cre-lox circularization in E. coli

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    Cre
    

    Use

    Synthetic Biology

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115270

    Purpose

    Expresses TtCsm6 from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    TtCsm6
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMaCTag-P07

    Plasmid

    #124788

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pR0306 CcaCas13b His6-TwinStrep-SUMO

    Plasmid

    #182687

    Purpose

    Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    CcaCas13b
    

    Tags

    SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMaCTag-P23

    Plasmid

    #120034

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Halo
    

    Use

    Pcr template

    Tags

    Halo

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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