We narrowed to 102 results for: cas12a

1. pMaCTag-P13

   Plasmid

   #120024

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mCherry
   

   Use

   Pcr template

   Tags

   mCherry

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMaCTag-P05

   Plasmid

   #120016

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   eGFP
   

   Use

   Pcr template

   Tags

   eGFP

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMaCTag-P34

   Plasmid

   #120030

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   3xFLAG
   

   Use

   Pcr template

   Tags

   3xFLAG

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMaCTag-P28

   Plasmid

   #120039

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   3xHA
   

   Use

   Pcr template

   Tags

   3xHA

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115211

   Purpose

   Expresses PsmCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PsmCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115267

   Purpose

   Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   LbuCas13a
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115219

   Purpose

   Expresses PspCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PspCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 21, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pRDA_816

   Plasmid

   #201157

   Purpose

   EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   5x-ALFA tag-dEnAsCas12a-VP64
   

   Use

   CRISPR and Lentiviral

   Mutation

   D908A

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVE13300

   Plasmid

   #137715

   Purpose

   Expresses impLbCas12a in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   impLbCas12a
   

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p46Cpf1-OP2

    Plasmid

    #98592

    Purpose

    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.

    Depositor

    Qiong Wu

    Article

    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:

    Insert

    FnCpf1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    araBAD

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pN251

    Plasmid

    #188471

    Purpose

    Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    enCas12a-HF (gift from Dr. Keith Joung)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRC10-EFS-huLbCpf1-2A-Blast-WPRE

    Plasmid

    #123359

    Purpose

    Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. U6-5'+3' MS2-CircRNA

    Plasmid

    #213752

    Purpose

    For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLQ10844

    Plasmid

    #183956

    Purpose

    CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    HyperdCas12a
    

    Use

    CRISPR

    Tags

    HA, Hygromycin Resistance, and mCherry

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ10875

    Plasmid

    #183963

    Purpose

    CBA-hyperdCas12a-HA-KRAB-mCherry

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    HyperdCas12a
    

    Use

    CRISPR

    Tags

    HA, KRAB, and mCherry

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CBA

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLQ10878

    Plasmid

    #183964

    Purpose

    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    HyperdCas12a
    

    Use

    CRISPR

    Tags

    HA, Hygromycin Resistance, and mCherry

    Expression

    Mammalian

    Mutation

    D156R, E292R, D235R, D350R

    Promoter

    CAG

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRDA_221

    Plasmid

    #169142

    Purpose

    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

    Depositor

    John Doench, David Root

    Insert

    EGFP , EGFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRDA_174

    Plasmid

    #136476

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    EnAsCas12a
    

    Use

    CRISPR and Lentiviral

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRDA_112

    Plasmid

    #136475

    Purpose

    Lentiviral expression of AsCas12a

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    AsCas12a
    

    Use

    CRISPR and Lentiviral

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRDA_052

    Plasmid

    #136474

    Purpose

    Lentiviral expression of AsCas12a gRNA

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    Puromycin resistance
    

    Use

    Lentiviral and Synthetic Biology

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge