164,826 results

1. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-a and hU6

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLJC5-Tmem192-3xHA

   Plasmid

   #102930

   Purpose

   Express a lysosomal Tag

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   Tmem192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUMVC

   Plasmid

   #8449

   Purpose

   Packaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLX_305_Ovalbumin

   Plasmid

   #184924

   Purpose

   Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

   Depositor

   Arlene Sharpe

   Article

   Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

   Insert

   Ovalbumin (OVAL Chicken)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Our vector has an A188T mutation that does not im…

   Promoter

   PGK

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 35S:RUBY

   Plasmid

   #160908

   Purpose

   RUBY under the control of CaMV 35S promoter/marker for transformation

   Depositor

   Yunde Zhao

   Article

   He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

   Insert

   RUBY and CaMV 35S promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available Since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-pegRNA-GG-acceptor

   Plasmid

   #132777

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   Exchangeable cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV(CAT)T7-SB100

   Plasmid

   #34879

   Depositor

   Zsuzsanna Izsvak

   Article

   Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

   Insert

   SB100X transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEcgRNA

   Plasmid

   #166581

   Purpose

   CRISPR-Cas9-assisted genome editing in Escherichia coli

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-MAPPER

   Plasmid

   #117721

   Purpose

   MAPPER Selectively Labels ER-PM Junctions

   Depositor

   Jen Liou

   Article

   Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

   Insert

   MAPPER
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL2-p21 promoter-Luc

    Plasmid

    #33021

    Depositor

    Martin Walsh

    Article

    Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

    Insert

    p21 promoter (CDKN1A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    p21

    Available Since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. ADORA3-Tango

    Plasmid

    #66212

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADORA3 (ADORA3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. [#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient

    Plasmid

    #233506

    Purpose

    Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    DsRed loxp eGFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFGET19_Ulp1

    Plasmid

    #64697

    Purpose

    H6-Ulp1 from pHYRS52 into pET-28b(+)

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Insert

    ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #168997

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1 - TRC cloning vector

    Plasmid

    #10878

    Purpose

    3rd generation transfer plasmid

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFR-GFP

    Plasmid

    #32751

    Purpose

    Mammalian expression of epidermal growth factor receptor (EGFR) fused to EGFP

    Depositor

    Alexander Sorkin

    Article

    Carter et al J Biol Chem. 1998 Dec 25;273(52):35000-7

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. SPLICS LY-MT Long P2A

    Plasmid

    #213613

    Purpose

    Detect the long-range Lysosome-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

    Insert

    SPLICS LY-MT Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SPLICS LY-MT Short P2A

    Plasmid

    #213614

    Purpose

    Detect the short-range Lysosome-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

    Insert

    SPLICS LY-MT Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6-tevopreq1-GG-acceptor

    Plasmid

    #174038

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET29b-10xHis-Super TEV

    Plasmid

    #193833

    Purpose

    Expresses high-efficiency TEV protease in bacterial cells

    Depositor

    Florence Pojer, EPFL Protein Production and Structure Core Facility (PTPSP)

    Article

    Lau et al protocols.io, doi: 10.17504/protocols.io

    Insert

    TEV protease
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

    Promoter

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

    Plasmid

    #188768

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. DHB-mVenus

    Plasmid

    #136461

    Purpose

    Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

    Depositor

    Tobias Meyer, Sabrina Spencer

    Article

    Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

    Insert

    DNA Helicase B (HELB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mutation

    Promoter

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ER-GCaMP6-210

    Plasmid

    #86919

    Purpose

    Fluorescent reporter for ER calcium signaling

    Depositor

    Timothy Ryan

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    ER-GCaMP6-210
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

    Promoter

    CAMKII

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-pAG-Hia5

    Plasmid

    #174373

    Purpose

    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein AG - hia5
    

    Use

    Tags

    6xHis and Protein A/G

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. prhaBAD-anti-rabbit-Nb-Hia5

    Plasmid

    #239627

    Purpose

    Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

    Depositor

    Aaron Straight

    Article

    Sidhwani et al bioRxiv 2025.07.01.662447; doi: https://

    Insert

    Hia5
    

    Use

    Tags

    6HIS, MBP, TEV, and anti-Rabbit Nanobody

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available Since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21a-BirA

    Plasmid

    #20857

    Depositor

    Alice Ting

    Article

    Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    BirA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV2/6

    Plasmid

    #240485

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid protein

    Depositor

    Meghan Rego, Addgene Research Program

    Insert

    AAV2 Rep and AAV6 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pLV-hTERT-IRES-hygro

    Plasmid

    #85140

    Purpose

    Lentiviral expression of hTERT, used to create immortalized cell lines

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAGGs-RFP

    Plasmid

    #127347

    Purpose

    Expresses RFP constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Red Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS Promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40

    Plasmid

    #105540

    Purpose

    AAV expression of EGFP-Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP-Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLX_305_OVA_Blasticidin

    Plasmid

    #238124

    Purpose

    Enables expression of Ovalbumin with Blasticidin selectable marker.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

    Insert

    Ovalbumin (OVAL Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAS_4xMma PylT_GFP 150TAG 6xHis

    Plasmid

    #140015

    Purpose

    Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Flag-Rab5A

    Plasmid

    #28043

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab5A (RAB5A Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET 28 full-length human PARP1

    Plasmid

    #192635

    Purpose

    Expresses human PARP1 with an N-terminal His-tag

    Depositor

    John Pascal

    Article

    Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

    Insert

    Human PARP1 (PARP1 Human)
    

    Use

    Tags

    histidine-tag

    Expression

    Bacterial

    Mutation

    Polymorphism A762

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-Frankenbody-FLAG-mEGFP

    Plasmid

    #175293

    Purpose

    Track mature and nascent FLAG tagged proteins in live cells

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CROP-seq-opti

    Plasmid

    #106280

    Purpose

    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCS2+HyPer7

    Plasmid

    #136466

    Purpose

    Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TK4-EGFP

    Plasmid

    #239047

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #42230

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pmVen-DHX9

    Plasmid

    #211409

    Purpose

    mammalian expression of human DHX9 N-terminally fused to mVenus

    Depositor

    Helmut Pospiech

    Insert

    DHX9 (DHX9 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSBbi-Pur

    Plasmid

    #60523

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pInducer20

    Plasmid

    #44012

    Purpose

    Tet-inducible lentiviral vector for ORF expression

    Depositor

    Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pTransposon.CAG.CasRX-P2A-Blast

    Plasmid

    #212965

    Purpose

    Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    CasRx
    

    Use

    Transposon

    Tags

    P2A-Blasticidin resistance

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

    Plasmid

    #212961

    Purpose

    Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_backbone
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pQCXIP-GFP1-10

    Plasmid

    #68715

    Purpose

    Split GFP assay

    Depositor

    Yutaka Hata

    Article

    Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

    Insert

    GFP1-10
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA3 Flag TSC2

    Plasmid

    #14129

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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49. mCherry

    Plasmid

    #176016

    Purpose

    Mammalian expression of mCherry. Parton lab clone DFQ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pemiRFP670-tubulin

    Plasmid

    #136573

    Purpose

    Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP670-tubulin
    

    Use

    Tags

    emiRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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