9,542 results

1. pBAD-Flp

   Plasmid

   #122969

   Purpose

   Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

   Depositor

   Lydia Freddolino

   Article

   Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

   Insert

   Flp recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   Aug. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. hCathepsin L

   Plasmid

   #11250

   Purpose

   Mammalian expression of human cathepsin L

   Depositor

   Hyeryun Choe

   Article

   Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

   Has Service

   Cloning Grade DNA

   Insert

   cathepsin L (CTSL Human)
   

   Use

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 25, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. OpenCRISPR-1 sgRNA-16nt stem

   Plasmid

   #221566

   Purpose

   Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1 sgRNA 16nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. sfGFP-C1

   Plasmid

   #54579

   Purpose

   Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

   Depositor

   Geoffrey Waldo, Michael Davidson

   Article

   Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pEMG-GRIS

   Plasmid

   #225134

   Purpose

   For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

   Depositor

   Lars Blank

   Article

   Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pN3-MGA

   Plasmid

   #107715

   Purpose

   Expression plasmid for 3xFLAG-tagged MGA

   Depositor

   Guntram Suske

   Article

   Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

   Insert

   MGA (MGA Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pOpen_taq

   Plasmid

   #153315

   Purpose

   Bacterial expression of Taq polymerase

   Depositor

   Drew Endy

   Insert

   Taq polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available Since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. AICSDP-15:ACTB-mEGFP

   Plasmid

   #87425

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   ACTB Homology Arms with mEGFP-linker (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   March 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pOpen-Tthlig

   Plasmid

   #165569

   Purpose

   Taq DNA Ligase

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   Tth DNA Ligase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 25, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid

    #221567

    Purpose

    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 12nt stem loop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. AMPK-SPARK

    Plasmid

    #226255

    Purpose

    phases-of-separation based activity reporter of AMPK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pC013 - Twinstrep-SUMO-huLwCas13a

    Plasmid

    #90097

    Purpose

    Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a
    

    Use

    CRISPR

    Tags

    6xHis-Twin Strep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAAV2/11

    Plasmid

    #240486

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV11 capsid protein

    Depositor

    Addgene Research Program, Meghan Rego

    Insert

    AAV2 Rep and AAV11 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Pet28a_Human_H3.1

    Plasmid

    #42631

    Depositor

    Joe Landry

    Insert

    Human H3.1 (H3C1 Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV2/5

    Plasmid

    #232922

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    Rep2/Cap5
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCDH-CMV

    Plasmid

    #72265

    Purpose

    Express gene of interest under CMV promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGloSensor -42F cGMP Vector

    Plasmid

    #236806

    Purpose

    Express -42F cGMP in biosensor assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    -42F
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. mMaroon-C1

    Plasmid

    #54835

    Purpose

    Localization: C1 Cloning Vector, Excitation: 610, Emission: 652

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mMaroon

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. NanoLuc-CCNE1 Vector

    Plasmid

    #238665

    Purpose

    Express NanoLuc-CCNE1 Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. CCNE1-NanoLuc Vector

    Plasmid

    #238666

    Purpose

    Express CCNE1-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge