164,577 results

1. pCS2-mutFAKSPARK2

   Plasmid

   #195264

   Purpose

   express Tyr to Phe mutant FAKSPARK2

   Depositor

   Xiaokun Shu

   Article

   Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

   Insert

   Tyr to Phe mutant FAKSPARK2 sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCMV-intron myc Rab2 S20N

   Plasmid

   #46780

   Purpose

   expression of S20N Rab2a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB2 (RAB2A Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S20N dominant negative

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pJW1518

   Plasmid

   #62388

   Purpose

   pFA6a -mCherry-BirA::HIS5

   Depositor

   Jonathan Weissman

   Article

   Williams et al Science. 2014 Nov 7;346(6210):748-51. do

   Insert

   mCherry-BirA
   

   Use

   Yeast c-terminal tagging vector

   Tags

   mCherry-BirA

   Expression

   Mutation

   Promoter

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-GST-TRP1

   Plasmid

   #41603

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GST and yeast ADH1 terminator

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-GBP-RFP-kanMX6

   Plasmid

   #105160

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

   Insert

   GBP-RFP-kanMX6
   

   Use

   Tags

   GBP-RFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)

   Plasmid

   #44090

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   6xGly linker, ADH1 transcription terminator, KanM…

   Expression

   Mutation

   Promoter

   Available Since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-Tet3G blasticidin

   Plasmid

   #128061

   Purpose

   Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. gag/pol

   Plasmid

   #14887

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   gag / pol
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pML104

   Plasmid

   #67638

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. T7-VEE-GFP

    Plasmid

    #58977

    Purpose

    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    EGFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET29b-10xHis-Super TEV

    Plasmid

    #193833

    Purpose

    Expresses high-efficiency TEV protease in bacterial cells

    Depositor

    Florence Pojer, EPFL Protein Production and Structure Core Facility (PTPSP)

    Article

    Lau et al protocols.io, doi: 10.17504/protocols.io

    Insert

    TEV protease
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

    Promoter

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV(CAT)T7-SB100

    Plasmid

    #34879

    Depositor

    Zsuzsanna Izsvak

    Article

    Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

    Insert

    SB100X transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEcgRNA

    Plasmid

    #166581

    Purpose

    CRISPR-Cas9-assisted genome editing in Escherichia coli

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.1(+)-CMV-bArrestin2-TEV

    Plasmid

    #107245

    Purpose

    Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ARRB2
    

    Use

    Tags

    TEV(NIA), tobacco etch virus NIA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2/6

    Plasmid

    #240485

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid protein

    Depositor

    Addgene Research Program, Meghan Rego

    Insert

    AAV2 Rep and AAV6 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pLX_305_Ovalbumin

    Plasmid

    #184924

    Purpose

    Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

    Depositor

    Arlene Sharpe

    Article

    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

    Insert

    Ovalbumin (OVAL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Our vector has an A188T mutation that does not im…

    Promoter

    PGK

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCX420

    Plasmid

    #229211

    Purpose

    Cytoplasmic-targeting Csm complex plasmid

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    Csm1; Csm2; Csm3(RNase mut)-2xsfGFP; Csm4; Csm5; Cas6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCX459

    Plasmid

    #229214

    Purpose

    CRISPR array plasmid for MAP1B (Spacers 1-24)

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    CRISPR array plasmid for MAP1B (Spacers 1-24)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCROPseq-VEX-optimized-scaffold

    Plasmid

    #203321

    Purpose

    Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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