...LEU13 IGH@ immunoglobulin heavy locus DKFZp686C15213, IGH, IGH.1@, IGHDY1, MGC72071, MGC88774 IGHA1 immunoglobulin...diversity 7-27 DHQ52, IGHD727 IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy...variable 1-58 IGHV158, VH IGHV1-69 immunoglobulin heavy variable 1-69 IGHV1-E, IGHV169, IGHV1E IGHV1-8 immunoglobulin...-30-3 IGHV3-3, IGHV3303 IGHV3-30-5 immunoglobulin heavy variable 3-30-5 IGHV3-3, IGHV3305 IGHV3-33 immunoglobulin...-30-2 IGHV4-3, IGHV4302 IGHV4-30-4 immunoglobulin heavy variable 4-30-4 IGHV4-3, IGHV4304 IGHV4-31 immunoglobulin...diversity 1-1 IGHD11 IGHD1-14 immunoglobulin heavy diversity 1-14 (non-functional) DM2, IGHD114 IGHD1-20 immunoglobulin...heavy diversity 1-20 IGHD120 IGHD1-26 immunoglobulin heavy diversity 1-26 IGHD126 IGHD1-7 immunoglobulin ...

... our collection! AAV are distributed as high-quality and high-titer 100 µL solutions of purified viral...coinjected with pAAV-TREtight-mTagBFP2-B19G Ian Wickersham 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper...AAV1 pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin...AAV1 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter...AAV5 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter...AAV1 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin...AAV5 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin...

...Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance...transfer from chloroplasts to nuclei provides a high-light signalling mechanism. Nat Commun. 2017 Jun 29...Red genetically encoded dopamine sensor YdLight1 and RdLight1 An expanded palette of dopamine sensors ...to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage sensor to detect...organelle-localized). Browse or search the tables below highlighting a few popular categories of biosensors or browse...NEMO as calcium indicators with large dynamics and high sensitivity. Nat Methods. 2023 Jun;20(6):918-924...for Ex Vivo and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1. pii: ...

...pAAV-hSyn-dLight1.1 Syn dLight1.1 none Constitutive 1 Tian 111067 pAAV-CAG-dLight1.1 CAG dLight1.1 none Constitutive...pAAV-hSyn-dLight1.2 Syn dLight1.2 none Constitutive 1, 5 Tian 125560 AAV-CAG-dLight1.3b CAG dLight1.3b none...pAAV-syn-dLight1.3b Syn dLight1.3b none Constitutive 9 Tian 125707 pAAV-CAG-RdLight1 CAG RdLight1 none Constitutive...Dopamine Sensors dLight RdLight GRAB_DA GRAB_rDA GRAB_gDA Serotonin Sensors psychLight iSeroSnFR GABA Sensors...187179 pAAV_hSyn1_nLightG Syn nLightG none Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR...Constitutive 9 Tian 125708 pAAV-hSynapsin1-RdLight1 Syn RdLight1 none Constitutive 9 Tian 113049 pAAV-hSyn-GRAB_DA1m...none Cre dependent 9 Tian 163909 pAAV_hSynapsin_psychLight2 Syn psychLight2 none Constitutive 9 Tian GABA...

...constitutively express your gene of interest at high levels. Perhaps high expression leads to slow bacterial growth...pDawn 43796 FixK2 Blue Light (470 nm) Andreas Moeglich Empty vector for blue light inducible expression ... 43795 pR_FixK2 Blue Light (470 nm) Andreas Moeglich Empty vector for blue light repressible expression...protein of interest. pJT106b3 31393 Red light inducible inverter Red light (650 nm) Christopher Voigt When combined...PcpcG2 Green Light (532 nm) Christopher Voigt When combined with pPLPCB(S), encodes a green light responsive...for the production of large amounts of protein or high copy number plasmids. Whether you're studying the...individual protein’s function under a wide range of highly controlled conditions in vitro , or, if you want...

...the Fab fragments contain highly variable regions, shown in light blue and light orange, that bind to a ...are light-sensitive molecules which emit a specific wavelength of light after excitation by light of a...pair the brightness of the fluorophore with the abundance of the antibody target. If a bright fluorophore...molecular tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as...-efficient way to produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies...protein chains: two large heavy chains and two small light chains. These four domains come together to form... to be produced in a single individual, giving a high probability that an antibody will be able to recognize...

...Excitation and Emission maximum are listed in nm. Brightness is the product of exctinction coefficient and...low concentration but are prone to dimerization at high concentration, so this is noted here. Proteins that...Blue/UV Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 ...Top Cyan Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine ...Top Green Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids iLOV 447 497... Yellow Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids EYFP 513 527... Orange Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids mKusabira-Orange...

...Plasmids and Resources Plasmid Tools Collection Highlights Other Resources The zebrafish ( Danio rerio )...large populations that are amenable to genetics, high-throughput screening and non-invasive live imaging..., and drug discovery and development. Here we highlight some of the plasmids and tools available at Addgene... zebrafish gene or sequence . The table below highlights plasmids that contain tools for use in zebrafish...Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish Tools in our Collection CRISPR Please...control of gene expression in zebrafish using red light. LipoGlo - Steven Farber Lab. A reporter system ...lipoprotein abundance, size, and localization in high-throughput. MAZERATI platform - Leonard Zon Lab....

...joining (NHEJ) pathway and the less efficient but high-fidelity homology directed repair (HDR) pathway....also enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no ..., which are about the same size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from Faecalibaculum... downstream of the target site (termed left and right homology arms). The length of each homology arm ...available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...strands Prime editing and base editing both offer high efficiency and low indel formation compared to HDR...large-scale, forward genetic screening. CRISPR can make highly specific, permanent genetic modifications to eliminate...

...applications. Browse the Collection Highlights section to learn more. Highlights of Luciferase Tools in our Collection...derived from teLuc that emit yellow/green light and red light, respectively, for increased usefulness in...Luminescent Imaging with Nano-lanterns Collection Highlights Empty Backbones Expression Constructs Reporter...application. Firefly luciferase is considerably brighter than Renilla luciferase, but is more susceptible...their large size. NanoLuc® Luciferase is small, bright, and a secreted version of the protein is available...red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared range...pRSETb-pHlash : A pH sensor created by fusing a bright mutant of Renilla luciferase (Rluc8) through an...

...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV... to control cell activity in living tissue with light. Optogenetics is typically used in neuroscience ...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent...pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive ...Deisseroth 137158 pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...

...) is expressed at higher levels in mice than P1 bacteriophage Cre, facilitating high rates of Cre-driven...plasmids. Addgene's viral service provides high-quality and high-titer viral preparations of plasmids that...Cre-containing Plasmids Scientists have developed ways to tightly control Cre expression and to optimize Cre expression...to activate Cre. One advantage of this system is tight temporal regulation. Promoter-regulated Cre: The...pmCherry-CRY2-CreN CRY2-CreN and mCherry coexpression; Light inducible; Use with plasmid 26889. Mammalian Tucker...pmCherry-CIBN-CreC CIBN-CreC and mCherry coexpression; Light inducible; Use with plasmid 26888. Mammalian Tucker...62938 pET-(pos36)GFP-9xGGS-Cre-6xHis Cre fused to highly cationic GFP variant T7 Bacterial Liu 62939 pET-Cre...

... linker-Venus-6aa linker-Venus V5V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 5 amino acid linker V17V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 17 amino acid linker V32V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 32 amino acid linker VVV Anisotropy/brightness standard consisting of three Venus fluorescent...amino acid linker, respectively VVVV Anisotropy/brightness standard consisting of four Venus fluorescent...amino acid linker, respectively VVVVV Anisotropy/brightness standard consisting of five Venus fluorescent...connected via 5 amino acid linkers VVVVVV Anisotropy/brightness standard consisting of six Venus fluorescent ...

...(EXPRESs) Consortium You may also like... Gavin Wright Lab plasmids EXPRESs is a collection of mammalian...Institute Mitch Levesque University of Zürich Gavin Wright , Wellcome Sanger Institute Protocols: Video showing...extracellular receptor-ligand interactions. Kerr SJ and Wright GJ. J. Vis. Exp. (61), e3881, DOI: 10.3791/3881...O, Kwiatkowski DP, Duraisingh MT, Rayner JC and Wright GJ Nature 2011 Nov 9; 480(7378):534-7. Construction..., Adryan B, Thisse B, Thisse C, Teichmann S and Wright GJ Molecular & cellular proteomics 2010 Dec; 9(...neural leucine-rich repeat receptors. Söllner C and Wright GJ. Genome biology 2009; 10(9):R99. Large-scale..., Söllner C, Schuster-Boeckler B, Bateman A and Wright GJ. Genome research 2008 Apr; 18(4):622-30. A benchmarked...

.... In addition to being useful for our bodies to fight off intruders, antibodies are also extremely useful...to researchers in a lab. When an antibody binds tightly to a specific antigen, scientists can use it to...and Expressing Antibodies The following table highlights plasmid collections that can be used to create...Article PI One-step assembly of antibody heavy- and light-chain DNAs into a single mammalian expression vector...Biophys Res Commun. 2015 Jan 2;456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv...Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...

...collections for use in plants. The tables below highlight plasmids spanning a wide variety of molecular ...The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, ... Li. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and...Return to top Empty Backbones The table below highlights plasmids that can be used as empty backbones ...Return to top Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent...

...iRFP variants have been shown to have high effective brightness and allow for single or multicolor imaging...near or above 650 nm as signals below 650 nm are highly absorbed by hemoglobin and cause undesirable background...iRFPs can be ‘turned on’ by non-phototoxic far-red light and are used for spatially selective imaging of ...Vivo Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal...Crimson Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1... Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690/717 (after photoactivation...

...proteins can also be controlled by light, generally by utilizing a light sensitive protein from cryptochromes...plasmids encode proteins that are controlled by light. Many of these plasmids encode for microbial opsins...archaebacteria, that can be opened and closed in response to light. These proteins can be expressed in specific neurons...membrane channel that opens in response to blue light and allows cations to flow into the cell. Variants...cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV) proteins. These fusions can then be used as...or search the table below for plasmids that use light to control a protein or cellular activity. For example...

...mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson 55165 mCherry-ZO1-C-14 Tight Junctions ...vesicles Clathrin, light polypeptide mRFP Ari Helenius 70217 pEGFP-GFP11-Clathrin light chain Clathrin-coated...cell junctions: adherens junctions, gap junctions, tight junctions, and desmosomes. Browse or search the ...Beta-Catenin mCherry Michael Davidson 30313 pEGFP ZO1 Tight Junctions Zonula Occludens-1 GFP Alan Fanning *Fusions...-coated vesicles Clathrin light chain GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles...Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-Rab5 ...

...includes MTA facilitation, DNA amplification, and high-quality quality control measures such as titering...offer 960 in-stock viral vector preps, delivering high quality and ready availability at affordable prices...vector experience, from methods to troubleshooting insights, you make research more reproducible for scientists...Viral Vector Production Learn about how we produce high-quality viral vector preps at Addgene, including...techniques (Link opens in a new window) for producing high-quality research-grade viral vector preps. Data ... that complement published data, capturing the insights that often go unshared. Help Center Check out ...

... display less off-target activity. High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF,...95% of archaeal and 48% of bacterial genomes, are highly diverse, with varying PAM sequences and number/...) Type V (Cpf1) Type IV (Csf1) Type VI (Cas13) Fighting Back: Anti-CRISPR Genes in Phage The CRISPR adaptive...creating a double-strand break. This method displays higher efficiency than HDR-mediated editing. The first... Genome engineers continue to work to develop a highly specific, programmable platform well-suited for...MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK. 2016. High-fidelity CRISPR-Cas9 nucleases with no detectable...

...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...and T492V. AAV44.9 The AAV44.9 serotype displays highly efficient transduction and lateral spread in the...Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency transduction of the mouse retina by tyrosine-mutant...a mouse model of complete congenital stationary night blindness. Hum Mol Genet . Nov 1;24(21):6229-39....acknowledge Arun Srivastava and cite Ling, et al. 2016. High-Efficiency Transduction of Primary Human Hematopoietic...27759036 Other citations include: Ling, et al. 2016. High-Efficiency Transduction of Primary Human Hematopoietic...

... maintain this plasmid collection page, which highlights plasmids available that may be useful for COVID...called SARS-CoV-2. Scientists have been working at lightning speed to elucidate as much as they can about this... develop and optimize the method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK... now available from Xiaokun Shu's lab. A rapid, highly sensitive and open-access SARS-CoV-2 detection ...Transcriptase plasmids from Andrew Ellington's lab. High-surety isothermal amplification and detection of...

...that it is responsive to specific stimuli, such as light. On a broader scale, the expanded genetic code can...included. Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA synthetase...TAG Edward Lemke Browse Strains The table below highlights bacteria strains that have been modified to enhance...Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified codons...

...deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Wickersham Introduction to ...tracing virus that lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the...starter" cell, but new virus cannot spread to any neighboring cells. To enable monosynaptic spread of this ...newly formed viruses are unable to infect any neighboring cells. Thus, through complementation, G-deleted...released from the starter cell and free to infect neighboring cells via G-protein receptors, which are naturally...

...cancer cells. Adenoviruses have high transduction efficiency and direct high but transient transgene expression...clinical trials. These viruses have a relatively high transduction efficiency, though this infectivity... can be delivered to a host and generate virus (right), similar to how natural viruses can be delivered...stranded linear DNA >8 Kb 105 Dividing/Non-dividing No High γ-Retrovirus Stable RNA <8 Kb 80-130 Dividing Yes...

...for tetracycline transregulation. Dox binds with high affinity to tTA and rtTA; thus, dox can be used ...plasmids third-generation rtTA On Elledge 19407 pTREtight2 Promoter contains a modified TRE that is silent...silent in absence of binding tTA or rTA; has high copy E. coli origin None Either Ralser 64238 pTet-IRES-EGFP...Expresses firefly luciferase hairpin and GFP under pTREtight promoter None Either Elledge 35625 pAAV-Ptet-RFP-shR-rtTA...promoter and enhancer options tTA Off Uney References Tight control of gene expression in mammalian cells by...

... nLightR. A, Structural models of nLightG (left) and nLightR (right) generated using ColabFold (Mirdita...indicators: nLightG and nLightR (Kagiampaki et al., 2023).     Figure 1: Properties of nLightG and nLightR...al., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly...norepinephrine. nLight is 28-fold more selective than GRABNE, with both nLightG and nLightR showing significantly...experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly expand the toolbox... both nLightG and nLightR, and may soon have a red tool which matches the sensitivity of nLightG. Many...images of HEK293T cells and neurons expressing nLightG or nLightR before/after application of NE (10 μM) and...

... Viral Vector Packaging Service Addgene produces high-quality viral vector preps from select plasmids ... and lentiviral vectors as in-stock preps. These high-titer preps are distributed as 100 µL solutions ...particles that are suitable for in vivo use. Order a high-quality and ready-to-ship AAV or lentivirus prep...services to you, we must ensure that you have the right to use the original plasmid. Distribution of the...

...Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning...end-joining ligation fidelity and bias data to predict high accuracy junction sets for Golden Gate assembly.... biological materials and intellectual property rights, and provides liability protection for material...qualified for-profit entities. Addgene reserves the right to determine if an entity is qualified to receive...

...types and/or developmental timeframes. Here we highlight some of the plasmids and tools available at Addgene...Plasmid Tools for Use in Worms The table below highlights plasmids that contain tools for use in worm research...

...optimized for each specific virus in order to generate high quality preparations. After production, all virus...titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method...digital PCR (ddPCR). Quality Control Addgene ensures high quality viral vectors by optimizing and standardizing...staining or SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid proteins...

...mutations are known to cause Rett syndrome, however, eight recurrent missense and nonsense mutations (R106W... labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the MECP2 gene... 55–67. PMID: 30536762 Shahbazian et al. 2002. Insight into Rett syndrome: MeCP2 levels display tissue...

...that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target...prefers a 'G' at the transcription start site to have high expression, so adding this G could help with expression... culture unless the targeting efficiency is very high. How should I design experiments for multiplexed...but the efficiency will be lower, although this might still be okay if you are introducing a selection...

...will have the highest specificity. To increase specificity, you can also consider using a high-fidelity Cas...gRNA targeting 5′ exon or essential protein domains High-fidelity Cas enzymes increase specificity. Dual-...a mammalian cell line that can be transfected at high efficiency Lentiviral transduction Cas9 and gRNA...

...vectors with the highest specificity and brightness, assessed using ssRNAseq or high-resolution imaging...screened thousands of enhancers for specificity and brightness of fluorescent labeling across brain regions ...

...the lowest biosafety protection levels. High quality and high titer in-stock AAV preps are affordable,... density centrifugation. Addgene’s comprehensive high-quality quality control process includes titering...

...combination allows IPI to generate antibodies to highly conserved proteins and other difficult-to-target...part of that mission, IPI is providing access to high-quality, well-characterized antibodies in an open...) Glypican Antibodies Glypicans are a family of highly conserved proteoglycans crucial for regulating ...

...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element, making...oxidizes its substrate (luciferin), it generates light that activates the opsin to either excite or inhibit...

...joining fragment comprising a κ light chain constant domain (C L ) and the κ light chain polyA tail sequences... for light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain...in a new window) Durocher Y, et al. 2002. High-level and high-throughput recombinant protein production... R-mAbs are secreted into the culture media. For high level expression we recommend using the HEK-2936E...Link opens in a new window) . The schematic to the right shows the separate elements of the R-mAb expression...expression plasmid involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters...

... for each specific antibody in order to generate high quality preparations. Following production, all ...transfected with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and ...amplified with primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified...

...Vector Core) and Addgene have partnered to provide high-quality AAV vectors to the academic research community...Deisseroth AV-1-36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...James M. Wilson AV-1-PV2699 100030-AAV1 pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen...

... are able to recognize their targets with fairly high specificity. Fifteen years of research has shown...TALe research has been swift. The incentives are high. Since ZF targets are confined to sequences composed...on specificity is on par with ZF arrays, if not slightly lower. Figure 2: Simplified representation of ...

...and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq platform...dissemination to the research community. This collection highlights plasmids created with support from the BRAIN ...

...pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase...recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450 CN1851-rAAV-hI56i-minBglobin-iCre...

...hundreds of in-stock preps that are ready to ship right away, as well as our Packaged on Request service...expressing Rep2 and B1 capsid (backbone may yield higher titers than pAR-B1) Sena-Esteves 78503 pAR-B1(1986.1...systemic delivery Maguire 198014 pCMV-AAV2cap AAV2 For high-level expression of the AAV2 cap gene, suitable ...

... clones for loss of function. This is due to the high frequency of indels formed on non-deleted alleles...cells in order to enrich for cells that received high levels of the CRISPR/Cas9 constructs. Plate sorted...the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nature Protocols...

...Migration Consortium (CMC) and Addgene, which highlights CMC depositing labs and plasmids....

...resources for emerging targets in AD and creating high-quality, well-characterized lead molecules for prioritized...further translational investment. All resources and high-quality research tools are shared openly. Plasmids...

... plasmid with or without expression of Cas9. Highlighted below are some multiplex gRNA vectors from our...Yang A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and...