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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 780 results for: scr

Showing: 261 - 280 of 780 results

  1. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...to keep the audience engaged through a computer screen. For the Reproducibility for Everyone Workshop,...author of the poster,” he says.Viewing a poster on a screen is challenging, requiring you to zoom in to different... their poster, eliminating the need to zoom and scroll around the poster (Reshef et al., 2020).The Twitter...

  2. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...adoption of the technology. In 2012, Saito et al. described the first Nano-lantern, a fusion of a mutagenized...hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki et al. improve the brightness of previous...substrate furimazine. In addition to the previously described applications, the enhanced signal of eNL allows...

  3. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...27478040. The lung cancer mutant collection is described in this paper. Systematic Functional Interrogation...the genetic pathway reference collections are described in this paper Systematic morphological profiling...  The genetic pathway reference collection is described in this publication We also wrote an extensive...

  4. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9 Nissim et alTsai...transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9   Xie et al  ...

  5. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...expressing cell lines, which can then be used both for screens (using pooled CRISPR knockout libraries) and individual...monoclonal Cas9-expression line prior to performing a screen or even a single gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D... vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID...

  6. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...Cell, Musunuru, Chad Cowan and their colleagues describe a much more efficient tool for doing that disease...modeling work in human pluripotent stem cells: a transcription activator-like effector nuclease (TALEN) kit...people unfamiliar with this new TALEN kit, can you describe it for us? Musunuru: TALENs are a type of genome...

  7. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...formulation of new knowledge than it is to read a manuscript whose whole purpose is to provide detailed evidence...images, noises and music, spoken words, and any onscreen text. Be aware of the effort the audience is expending...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just...

  8. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...Systems Biology in November, Voigt and Alex Nielsen describe what’s possible when multi-input CRISPR/Cas genetic...many deposits to Addgene over the years. Can you describe the toolbox you are putting together?  Voigt: ... Those will allow you to set the amount of transcriptional and translational activity a cell has and control...

  10. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...neurons from each class to fully characterize the transcriptome. The first data release for CeNGEN is planned... The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans. To study the role...Addgene In addition to the wealth of open resources described above, there were many plasmid tools presented...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...'ve overlooked regulatory fragments of these transcripts. I remember when small RNA was just something...’t give tRNAs much thought. High throughput transcriptome sequencing methods including work done by the...circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As...

  12. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...histone modifications, which can influence the transcription of genes by RNA polymerase II, or by the addition...methylcytosine in CpG islands makes  genes less transcriptionally active. The role of epigenetics in studying...of tumor suppressor genes can repress their transcription and inactivate them. Global hypomethylation,...

  14. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...chromoproteins have a shorter history. In 1987, scientists described an unusual blue-colored protein from the jellyfish...methods. While techniques such as  blue-white screening require X-gal, an expensive exogenously-added ...chromoproteins don’t require other substrates for screening. Thus chromoproteins can be used as markers in...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...synthesize a particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated...template of choice and allowing these cells to transcribe, translate and modify your protein of interest...assembled in vitro using purified components of the transcription and translation machinery. These include ribosomes...

  16. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...transfer plasmid at 30 °C instead of 37 °C and then screen for ITR recombination with a SmaI or XmaI restriction...duplicated regions like the ITRs. Restriction digest screening for ITR deletion is still necessary. To save time...mL of my AAV transfer plasmid cultures and then screen with SmaI digests. I freeze the bacterial pellet...

  17. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)...identification, we developed custom open-access Python scripts that can perform a deeper analysis of the data....Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS...

  18. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...following cloning methodologies have been extensively described in other articles; so if you are interested in... to assemble up to 6 different fragments in a prescribed order. These fragments can be pooled in one tube...homology. You might also consider FastCloning, first described by Chaokun Li and colleagues. Here you simply ...

  19. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...vivo. A common choice for conducting genome-wide screens using CRISPR. AAV transduction Only compatible...containing gRNA and Cas9 are used in in vitro transcription reactions to generate mature Cas9 mRNA and gRNA...complexes Purified Cas9 protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex...

  20. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button...into two components: 𝜃x(t) and 𝜓z(t). 𝜃x(t) describes the exponential decay process we observed, while...appears to return to normal. The model fit these two discrete phases with an estimate of 62.3% for the COVID...
Showing: 261 - 280 of 780 results