...author do not represent a formal stance taken by Addgene or its staff. In Washington, D.C. as well as sister...rights (2). Less formally, governments are the organizations that make and enforce the rules of the lands...climate change, the safety of genetically modified organisms (GMOs), the absence of a link between vaccines... while their website lists over 200 partner organizations, and 514 satellite marches are scheduled to ...author do not represent a formal stance taken by Addgene or its staff. Many thanks to our guest blogger,...Chronicle of Higher Education: “March for Science” Organizer Says It’s About the Public, Not the Scientists...

...Browse Addgene's collection of luciferase plasmids for assays of transcriptional regulation and bioluminescent... bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects... The most common types of luciferase found in Addgene’s collection are Firefly luciferase (Fluc), Renilla...interest before or after luciferase . In addition, Addgene has many luciferase expression constructs available...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR to a given...expert to see results! Getting Started with CRISPR Addgene has empowered researchers to further develop the...applications. For more information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR... CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models: Mammalian systems...

...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...making it easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed ...systems The ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, Mathieu, et al. (2015). “A Scalable...26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and ...57. PMID: 26701308. Additional Resources on the Addgene Blog Brush up on Homology Directed Repair and ...

...academia and industry. As a result over 90% of Addgene’s COVID-19 resources are now available to scientists...these groups were willing and able to listen to Addgene’s new approach of working with the industry-based...in the background became a normal occurrence as Addgene and our university partners quickly assessed how... happy to have the support of the following organizations to share materials more broadly to accelerate...allowed them to synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us...COVID-19 plasmid collection Beyond COVID-19: Addgene’s plasmid collections available to industry scientists...to industry? Over the past 8 years, several organizations have made their plasmids available beyond academia...

...touch! Resources at Addgene Read Our Posts on Networking Get Career Advice from Addgene Bloggers Learn How...and some of the great biological societies that Addgene has had the opportunity to work with at Freshers...support each other and provide advice. The society organizes numerous recreational and educational events throughout...outside academia. Inspiring future scientists At Addgene, we’re incredibly glad to see these biological ...society or event which would benefit from some Addgene support? Please get in touch, as we aim to support... How to Get Involved in a Profession Organization ...Cambridge and within the general public. The society organises talks by prominent scientists, including Nobel...

...that expound on bench skills and experience. Most Addgene PhD level job descriptions clearly describe that...send directly from a mutual connection. If the organization is small, any person at the company might be...be able to help you. If the organization is large, you will need to be more directed and target someone....  You might also enjoy the other posts in the Addgene Blog Scientist Careers Series.    ...

... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...many cases, however, a GFP-tagged cell line or organism has already been made, and it would be expensive...already have created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding one of the... GFP is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used...PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis...

...Resources at Addgene.org Find careers at Addgene Watch out career videos Sign up for the Addgene quarterly...making this transition. In this installment of the Addgene career guide, we’ll think about what causes this...job candidate for a senior scientist position at Addgene, the skeptical applicant asked if she would partake...of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list of things... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...the years that one of the things I enjoy about Addgene is that I’ve never been bored. There is always ...someone who coordinates cross-team projects at Addgene, I find that my vocabulary improves on a weekly...

... a Professional Organization Resources on Addgene.org Check out Career's at Addgene ... the Management Team that steers the organization. As an Addgene vet familiar with the expertise and skills...joined Addgene’s Green Team, a like-minded group of co-workers who want to make our organization as environmentally...unless we manage to make Addgene the most environmentally friendly organization on the planet, in which...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting... by some colleagues as the “cross-team guy” at Addgene, an unofficial title that I happily embrace (and...possibly understand every nuance of how kits work at Addgene, but with the help of these people and others, ...

...facing a financial crisis. As a nonprofit organization, Addgene charges enough for our services to run our...funding organizations to request support for our operations until our normal work resumed. Addgene distributes...materials via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with a small... all over.” And we sent the Addgenies home.  At the same time, as Addgene started dealing with this new...resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure...science sharing as we can get. The effects of Addgene’s brief shut-down and the subsequent fluctuations...don’t have space in this blog to call out every Addgene Team because they’re all doing amazing work during...

... Stopwatch! Any organization that deposits with and/or requests materials from Addgene can qualify. We...between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene Master Reception...between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA: material ...question? Refer them to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the...commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology Transfer...arrival of the newest plasmid you requested from Addgene...the plasmid that will help you run that last ... for depositor and requestor alike. That’s why Addgene works closely with TTOs to help protect our depositors...

...Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation...and have the capacity to give rise to an entire organism. iPSC technology removes the possible ethical ...may be difficult to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs...know which method you would like to use, browse Addgene's collection of Reprogramming Plasmids in the table...cells or fully differentiated cell types. Browse Addgene's collection of Differentiation Plasmids below. ...inherent disadvantage of iPS cell technology. Browse Addgene's collection of Transdifferentiation Plasmids below...below will help you find plasmids available from Addgene that can be used to create iPSCs or another cell...

...FOP until a cure is available. Addgene and AJ's Avengers This year Addgene, where my wife Lianna has made...their plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the ...years. Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers... Disease that runs on behalf of the National Organization for Rare Disorders (NORD). NORD raises money...helping us fight against FOP in two ways. First, Addgene is distributing ACVR1 Plasmids from a variety of...We will be collecting donations for IFOPA, an organization dedicated to raising funds and awareness of ...Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease...

...and PNS Resources on Addgene.org Find the Spaghetti Monster probes at Addgene Browse all AAV vectors...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...University. Find the Spaghetti Monster probes at Addgene Uses of smFPs 1. Multi-channel connectomic tracers...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...PubMed PMID: 18057027. Additional Resources on the Addgene Blog Rabies and neuronal tracing Retrograde AAV...

...Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page Find... editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms...obligate thermophiles without the need to grow the organisms at lower than optimal temperatures. GeoCas9’s ...try, as always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas...bioRxiv (2017): 138867. Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome... Find CRISPR plasmids at Addgene ...

...below and an Addgenie will help you find what you need.   More Plasmid Resources from Addgene: Plasmids... is which vector will best suit your needs? At Addgene, we have a vast collection of empty backbones that...your expression system or environment. The host organism will determine the type of vector that you will...your plasmid has been incorporated into the host organism, usually achieved with the proper selection marker... of the specific mutations. For these purposes Addgene has a large collection of yeast expression vectors...Plasmids 101 blog series Addgene's Molecular Cloning Guide & Other Plasmid Protocols List of common epitope ...

...skills”. Leadership is prized at all levels of an organization and is also one of the most common criteria ...work alone much of the time, depending on the organization they lead. Effective leaders rarely give orders...series of blog posts in one pdf - download the Addgene Management for Scientists Ebook.  Fire, Snowball...

...Dimers and Multimers" post. It's also been re-organized and updated with a fresh new look.  We think this...plasmids, they don’t go to Wikipedia; they go to Addgene.”-Ryan Mehl, Professor, Oregon State University...

...grow liver stem cells and liver-like mini-organs or organoids, neither of these can substitute for a liver...career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our career videos ...talk preparation: Define your research vision. Organize your vision into a chalk talk format. Practice...questions from open ended screens and data collection. Organize your research vision into the chalk talk format...outdoor photographer.  Additional resources on the Addgene blog Find tips for presenting a science talk Check...

... viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral...and space. Zebrafish have become a popular model organism because their larval stage lends itself well to...toolbox and the versatility of the zebrafish model organism, the Isacoff lab was able to link these cells ...PMCID: PMC2770190.  Additional resources on the Addgene blog Find all our optogenetics blog posts Read...

...Genetic Engineering Resources on Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory...and is relatively easy to grow, it is an ideal organism for teachers looking to expand their science curriculum...professional education associations, and local organizations such as the Center for Science and Industry ...-12 education.     Additional Resources on the Addgene Blog Learn how BioBuilder can bring Synthetic ...

... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...The best trap depends on your experiment and the organism you are working in. The spacing of genes and whether...integration, transposon, and many more! Check out Addgene’s catalog to find trapping tools for your next experiment...Plasmids 101 e-book Additional resources on the Addgene blog Plasmids 101: Using Transposons in the Lab....1105/tpc.12.7.1007  Additional resources on Addgene.org Vector database Intro to the Lab Bench Plasmids...

...collaboration.  In that post, we talked about Addgene’s data processing needs, our criteria for selecting... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

...on the Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing Tips for...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out Our Hot Plasmids Articles to See Compiled...

...abundant biological dark matter and so the lab organized a sampling trip to Yellowstone National Park (...then, many researchers have searched for novel organisms and enzymes from these extreme environments. Gearing...particularly useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of...GeoCas9, another thermostable Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

... Additional resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

...For example, the many resources available on the Addgene website didn't come into being through the actions...develop to reach their full potential in the organization. This will result in more engaged, successful... Managerial Skill" Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete... Individual Development Plan Resources on the Addgene Website Are You a Molecular Biologist? Find Plasmids...

...a positive stir and was recognized by meeting organizers in their town hall.  Iterative improvement - ...Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance than others... with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons to...take some leaps. Binning Singletons is its own organization, working to make scientific meetings more productive...Binning Singletons, Inc. Additional resources on the Addgene blog Browse our blog posts on scientific conferences...

...Read Other Inside Addgene Posts Resources on the Addgene Website Meet the Addgene Management Team Join...Additional Resources on the Addgene Blog Get Management Advice from Addgene Executive Director Joanne ... are needed. All-Company Meetings: As your organization grows, so will the length of your group meetings...meetings. Make sure to keep them manageable. At Addgene, teams can add as many points as they like to the...the meeting, take notes on the agenda points. At Addgene, we like to do this live on the screen using a ...review or project plans, before the meeting. At Addgene, we like to send around a Google doc where people...provided some tips above for what works well at Addgene, but meetings are never one-size-fits all. Feel...

...CRISPR plasmids for that particular organism available at Addgene. Percentages represent the fraction ... are members of the Addgene Advisory Board. Additional Resources on the Addgene Blog Get the Most from...available to the academic research community. At Addgene, we’ve helped distribute many of these technologies...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous...Biologically Inspired Engineering and member of the Addgene Advisory board, on the difficulty of producing ...John Doench (Broad Institute and member of the Addgene Advisory Board) says: "When you're setting up to...Getting these quality-controlled reagents from Addgene can greatly expedite a researcher's ability to ...

...tools. Find the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098...small, and their transparency allows for whole organism imaging. They created an endogenous ApoB-NanoLuc...cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International Journal of...systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications, 2019. PubMed...worms and have made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as...were larger than previously reported for other organisms, and the Gottschalk group hypothesizes that this...biosensors at Addgene. Hashemi et al., PNAS. 2019, PubMed PMID 31371514. ...

...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...gestures are memorable! Attend events for one organization or go to the same conferences/meetings over ... up will be easy. Additional resources on the Addgene blog Browse other blog posts on networking Attending...

...sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene depositors who study chromatin...associated PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin...pii: 1722491115. Addgene blog posts related to chromatin This is not the first Addgene blog post about ... 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are...phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate...Keystone Chromosome Architecture and Chromosome Organization. These aren’t the only investigators in the ...the chromatin field who have deposited with Addgene, but you’ll find many great tools by clicking on the...

...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, ...resources on the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to ...all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse the entire plasmid collection...investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional...2020). The authors requested these plasmids from Addgene. This publication then resulted in the development...3X-Flag-SARS-CoV-2 S, which is now easily available through Addgene and has already been requested dozens of times ...develop available to the scientific community. Addgene was awarded a Fast Grant to ensure that the scientific...

...genome-wide, and has been widely applied in many model organisms and cell lines. Although ChIP-seq is a relatively...cells. However, when working on specific tissues or organs in whole animal models like Drosophila melanogaster...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids...

... C. elegans. Find the split BoTN/B plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed PMID: 30704911...the body. When a microbe travels through a host organism, the expression of some genes can change depending...protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow dissolved...problematic for imaging. Find the ARG plasmids at Addgene. For more details on acoustic reporters, check ...assembly step. Find the miRNA reporter collection at Addgene! Gam JJ et al., Nat Commun, 2019. PubMed PMID: ...plasmids at Addgene. LaFleur et al., Nat Commun. 2019, PubMed PMID: 30971695. ...in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment...

...split base editors. Find the split base editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020...the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893...been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...Vasudevan et al., 2018. Find the CyanoGate kit at Addgene. Vasudevan et al., Plant Physiol 2018. PubMed PMID...2019. This novel tool is practical in cell and organ culture, as well as cell lineage studies. Eisenhoffer...Find CreLite at Addgene. Yen et al., bioRxiv 2019. DOI: 10.1101/823971. ... Various Addgenies...

...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great...plasmids or plant expression systems available from Addgene. If you have any questions or thoughts about this...PubMed. Find the plasmids from this publication at Addgene Other Techniques for gRNA Multiplexing Multiplexed....  Find the plasmids from this publication at Addgene Dimeric CRISPR RNA-guided FokI nucleases for..... Find the plasmids from this publication at Addgene ...

...in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op from Northeastern...used to generate mutations in a wide range of organisms, but its potential to efficiently correct disease..., however, because the disease affects multiple organ systems. Nevertheless, the findings are proof-of-principle...functional correction of the gene in clonally expanded organoids. “CRISPR in adult stem cells has huge potential...potential, because the genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...