We narrowed to 142 results for: endog
-
TypeBlog Post...pseudotyped with a mix of VSV-G and the baboon endogenous retrovirus Rless glycoprotein (BaEVRless) had...
-
Plasmids 101: FLEx Vectors
TypeBlog Post...mutations and truncations in the absence of the endogenous gene. Spatio-temporal expression of optogenetic... -
Antibody Production
TypeCollection... been retained. If the target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody)... -
Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...Finger Arrays Engineered Zinc Finger Arrays for Endogenous Zebrafish Gene Targets Recent published reports... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...CAPTURE CAPTURE 2.0 Biotinylation enzyme BirA Endogenous biotin ligases Expression system Stable cell... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging in Human Cells Check Out the Michael... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...Western blotting and PCR, depending on the level of endogenous expression. We highly recommend the use of an... -
Of Myc and Men
TypeBlog Post...impossible if you wanted to distinguish between endogenous and exogenous versions of the same target. In... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post... level observed will be dependent on both the endogenous promoter and the 3’ UTR of the locus of interest... -
CRISPR Guide
TypeGuide...Several labs have also controlled Acr activity with endogenous cell-type specific miRNAs to restrict CRISPR ...Validate genetic modification ). Browse Plasmids: Endogenous Tagging CRISPR Base Editing To overcome low HDR..., and the break can be repaired by the cell’s endogenous repair mechanisms. Figure 14: Overview of editing...repression gRNA G uide RNA , a synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both...Jaenisch, R. (2013). Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature . 628 (8008... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...then tested the RESCUE system’s ability to edit endogenous transcripts finding up to 42% editing efficiency... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...from a template molecule, which is usually an endogenously available sister chromatid, though exogenous... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post... this using Cre-Lox mouse models in which the endogenous MECP2 gene was disrupted and silenced by a Lox-Stop... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...efficiency is correlated with codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...Agrobacterium lacking the GUS gene since plants can have endogenous GUS-like activity (Hu et al., 1990). We commonly... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system." Proceedings of the National... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...new effector (Abudayyeh et al., 2016). In the endogenous CRISPR/Cas9 system, Cas9 uses both a tracrRNA... -
CRISPR Activation: A Practical Guide
TypeBlog Post...light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11:198–200 . https... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...gram negative bacteria periplasm. Efforts to tag endogenous genes for proteins in these compartments using... -
Plasmids 101: Control Plasmids
TypeBlog Post...produce a similar product, but do not target an endogenous gene in your experimental cells. For example,...