...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...glycoprotein. The pseudotyped protein is encoded on the envelope plasmid which contains a promoter, the envelope... spike protein, scientists encode the spike protein on the envelope plasmid. Image from Crawford et al...with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of...

...Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector...vector from the Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is a technology that can be...vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...separate envelope and packaging plasmids as well as transfer plasmids. This concept ensures safe handling...gamma-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, may be...Learn more about CRISPR plasmids for transcriptional inhibition. Retroviral Plasmid Kit Retroviral Barcoding...expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by The RNAi Consortium...

... heavy chain proteins as well as the genes that encode those proteins are designated by the lower-case...co-expressing IgM and IgD isotypes antibodies which are encoded by the heavy-chain constant genes μ and ẟ. These...Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

...cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing the...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...Development and characterization of sindbis virus with encoded fluorescent rna aptamer spinach2 for imaging of...for quantitative imaging, Ming Hammond’s group created Spinach2 using site-directed mutagenesis of Spinach...was cloned into bacterial expression vectors to create a library. Bacteria were transformed, and then ...for in vivo studies. Dimerization of Broccoli increases its fluorescence by about 1.8-fold, and may be...

...Flp-dependent Cre AAV in AAV9 and AAVrg. See our new additions here: Cre vectors pAAV-EF1a-fDIO-Cre (121675...is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available as 20...expression. This is a Cre-dependent switch that expresses nuclear mCherry in the absence of Cre, and expresses...expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium sensors and GABA sensors) Calcium sensors... technology, see our blog post Overview of AAV Encoded Calcium Sensors). We are expanding the inventory...sensor (see below), in addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV...reduced. This variant has an approximately 12-fold increase in brightness after ~100 action potentials. This...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...CRISPRi system, the Laub lab repressed ctrA, a gene encoding an essential cell cycle regulator. The lab expressed...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...

...Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is comprised...ERK, or a backbone to use for custom KTR design, plasmids are available from Addgene! References Kudo, ...protocols, 13 1, 155-169. PMID: 29266096. Find the KTR plasmids here! Additional Resources on the Addgene Blog...Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors ... key advantage of KTRs is that they are easy to create and simple to multiplex. Problems with FRET kinase...

... cas1, cas2, cas4, and a new gene cas14. cas14 encodes for a small Cas protein (40-70 kDa), which is half...regions containing the putative tracrRNA into a plasmid and expressed it in an E. coli bacteria strain....sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR guide page Browse the...diagnostic toolkit, called DETECTR, which has been created by the authors. This kit uses Cas12 and Cas13 to...

...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...following descriptions for your plasmid: Encodes insert(s) A plasmid with one or more genes, protein...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose... Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered ...

... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...already have created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding one of the... Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM,...Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent Protein Should I...characterized, it turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson...necessary to observe a signal. Red signal intensity increases with GFP concentration and saturates around 30...