.... They can also be introduced directly into a Drosophila S2 cell line stably expressing Cas9. This PCR...Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016...

...the Drosophila community as well. The pooled library, designed to be used with cultured Drosophila cells...Mol Biol. 2014. PubMed PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege ...expression backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter...rather than a mixed sample. TRIBE was developed in Drosophila, and further work is needed to assess its function...plasmids optimized for dCas9-VPR expression in drosophila and yeast. Chavez A, et al. Nat. Methods 2015...

...number of model organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria...

...increases knock-in efficiency. Finally, as shown in Drosophila S2 cells (Böttcher et al., 2014), inactivation...PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https...

...Halstead et al. used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, ...

... his calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all ...

...Is there any AAV serotype that can be used in Drosophila? A: Not to our knowledge. Disease Applications...

...genome engineering in species like C. elegans and Drosophila. Light inducible promoters Light is another way...

...which it was previously not an option, including Drosophila and C. elegans.   Find the TurboID plasmids here...

...includes systems for tagging mammalian cell lines, Drosophila cells, and C. elegans. In addition to generating...

...an open science genome engineering project in Drosophila. ...fly design is a genome engineering project in Drosophila, consisting of transgenic cas9 fly lines and ...1gRNA expression of gRNA under control of the Drosophila U6:1 promoter Bullock 49409 pCFD2-dU6:2gRNA expression... expression of gRNA under control of the Drosophila U6:2 promoter Bullock 49410 pCFD3-dU6:3gRNA expression...expression of gRNA under control of the Drosophila U6:3 promoter Bullock 49411 pCFD4-U6:1_U6:3tandemgRNAs ...3tandemgRNAs expression of two gRNAs from Drosophila U6:1 and U6:3 Bullock 73914 pCFD5 multiplex gRNA plasmid Bullock...SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster. Bullock 62208 pnos-Cas9-nos Expresses...

...GCaMP6 only). The genetic elements required for Drosophila studies have been added for GCaMP7 and GCaMP8...

...can be used to replace genes in yeast, mice, or Drosophila through homologous recombination. The concept...

...by members of the worm community but there are Drosophila and zebrafish articles published there as well...

... RNA targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The...

...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...modifications can be efficiently generated in Drosophila and transmitted through the germline. Using an...plasmids are described in: Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz...codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter. 45945 pHsp70-Cas9 : The codon-optimized...codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). ...expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter. 51019 pDsRed-attP : Vector...

...pCFD3-NS Drosophila Drosophila U6:3 pegRNA BbsI No Vermilion Norbert Perrimon pCFD5-NS Drosophila Drosophila...gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR...expression in mammalian systems, bacteria, plants, and Drosophila. Mammalian Plasmid Gene/Insert Promoter Selectable...Insert Promoter Selectable Marker PI Publication Drosophila Plasmid Gene/Insert Promoter Selectable Marker...

...Washington University whose research focuses on Drosophila-parasite pathogen interactions.  ...

...genes: deletion mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002...

...sources – voltage-gated potassium channels found in Drosophila to phosphatases from sea squirts. This design...