...fluorescent signals. Named for the "stellar explosion SUperNova," SunTag can help you turn up the brightness in... al. observed some GFP aggregation, which they reduced by using superfolder GFP (sfGFP) with the small... fusion could be used as well. Pros of SunTag compared to traditional fluorescent proteins Improved ...signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule...

...He et al also compared MG-2I-dL5** head-to-head with KillerRed, a genetically encoded red fluorescent photosensitizer...Fluorescent Protein Guide Page Check out MiniSOG2 and SuperNova, two other photosensitizers used for cell ablation...illuminated with green light. They found that KillerRed required longer exposure times (5 minutes vs 10 seconds...* also had higher photostability than KillerRed, with KillerRed reaching greater than 75% photobleaching...modified with the addition of two iodine residues. Compared to MG, MG-2I has increased ROS generation when...wavelength to 666 nm, which is within the near-infrared (NIR) spectrum (>620 nm). Above is a brief schematic...bleaching after longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell...

...85040 pK170.AAV-TRE-Cre-WPRE (Supernova) TRE-Cre based Supernova TRE AAV Iwasato 85577 pTC-CMV-Tet...Cre) Cre Tribolium casteneum hsp68 T. castaneum (red flour beetle) Averof 119868 pGEM-NLS-Cre-2A-mCherry-FKF...Mammalian Breunig 131780 pCS2+PIF6CreN CreLite - Red light-inducible Cre; N terminus Cre fused to PIF6...Zebrafish Eisenhoffer 131781 pCS2+PhyBΔCreC CreLite - Red light-inducible Cre; C terminus Cre fused to PhyBdelta... pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Cre activates your gene fused to eGFP, removes dsRed. See article...diversity Mammalian Megason 13769 pCALNL-DsRed Cre dependent DsRed expression Mammalian Cepko 13770 pCALNL-GFP...Reporter DsRED and EGFP Cre recombinase reporter Lentiviral Geijsen 65726 pLV-CMV-LoxP-DsRed-LoxP-eGFP...

...SunTag system, named after the "stellar explosion SUperNova", is a synthetic scaffold that recruits up to ...will appear yellow, but a translated mRNA will be red. This system achieves great specificity and resolution... and that fluoresce in the green (500-550nm) and red (570-620nm) portions of the spectrum. PhD student...transitions from emitting orange (λ= 565nm) to far-red (λ= 662nm) fluorescence after exposure to blue-light...Biology (2014) represent the newest versions of both a red- and a green-light switchable bacterial TCS system...aaRS pairs are delivered via a pseudotyped baculovirus system, which is advantageous compared to other viral... Good tools for measuring intracellular pH are required in order to determine the relationships between...