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Showing: 1 - 17 of 17 results
  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...promoter of lac and trp Regulated like the lac promoter Contains -35 region from trpB and -10 region from...crossover. Generally speaking, promoters in bacteria are less diverse and complex, having fewer parts than those...Cauliflower Mosaic Virus Constitutive  Active in dicots, less active in monocots, with some activity in animal...Constitutive  Murine U6 is also used, but may be less efficient.   Prokaryotic promoters     Promoter...expression through varied inducer concentrations. trp High levels of gene expression Promoter from E. coli...Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your...Constitutive  Tends to give consistent expression regardless of cell type or physiology. SV40 General expression...
  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...TetO elements, allowing transcription to proceed (GFP expression is controlled in this manner in pJKR-H-tetR...Researchers have engineered pADH1 variants that are less sensitive to ethanol, including the “middle” ADH1...the fungus Neurospora crassa. The QUAS promoter is less leaky than UAS in the basal state, and co-expression...tac promoter: a functional hybrid derived from the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(...
  3. Synthetic Biology - Yeast

    Type
    Collection
    ...10.1038/ncomms12211. PP295 Expresses GFP. Figure 3. Strain 1. GFP Yeast Expression Lu Synthetic biology...10.1038/ncomms12211. PP296 Expresses GFP. Figure 3. Strain 2. GFP Yeast Expression Lu Synthetic biology...10.1038/ncomms12211. PP297 Expresses GFP. Figure 3. Strain 3. GFP Yeast Expression Lu Synthetic biology...10.1038/ncomms12211. PP298 Expresses GFP. Figure 3. Strain 4. GFP Yeast Expression Lu Synthetic biology...10.1038/ncomms12211. PP299 Expresses GFP. Figure 3. Strain 6. GFP Yeast Expression Lu Synthetic biology...10.1038/ncomms12211. PP300 Expresses GFP. Figure 3. Strain 7. GFP Yeast Expression Lu Synthetic biology...10.1038/ncomms12211. PP318 Expresses GFP. Figure 3. Strain 5. GFP Yeast Expression Lu Synthetic biology...
  4. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ....0124958. eCollection 2015. MXS_PA-GFP MXS_chaining vector with PA-GFP PA-GFP (Synthetic) Synthetic Biology...expression. J Biol Eng. 2011 Sep 20;5:12. pBbB1a-GFP GFP (Synthetic) Bacterial Expression, Synthetic Biology...expression. J Biol Eng. 2011 Sep 20;5:12. pBbB1c-GFP GFP (Synthetic) Bacterial Expression, Synthetic Biology...expression. J Biol Eng. 2011 Sep 20;5:12. pBbB1k-GFP GFP (Synthetic) Bacterial Expression, Synthetic Biology...expression. J Biol Eng. 2011 Sep 20;5:12. pBbB2a-GFP GFP (Synthetic) Bacterial Expression, Synthetic Biology...expression. J Biol Eng. 2011 Sep 20;5:12. pBbB2c-GFP GFP (Synthetic) Bacterial Expression, Synthetic Biology...expression. J Biol Eng. 2011 Sep 20;5:12. pBbB2k-GFP GFP (Synthetic) Bacterial Expression, Synthetic Biology...
  5. Antibodies

    Type
    Collection
    ...128602 Nb-gp41-GFP (MoonTag-Nb-GFP) Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian...rerio) Wright 49172 pOPINE GFP nanobody Bacterial expression of GFP nano body GFP nanobody Collins 50365 ...(112) pcDNA3.1-GFP-Flag-Nup62-EPEA GFP and Flag tagged Nup62 with EPEA nanobody tag GFP-Flag-Nup62-EPEA...-NbVHH05-GFP Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly ...fly cells NbVHH05-GFP (Homo sapiens) Perrimon 171571 pAW-Nb127D01-GFP Expresses GFP-tagged anti-CXCR2 (human...cytomembrane CD8-NbVHH05-GFP (Homo sapiens) Perrimon 171577 pAW-CD8-Nb127D01-GFP Expresses GFP-tagged anti-CXCR2...
  6. Cre-lox system

    Type
    Collection
    ...48201 CAG-GFP-IRES-CRE Cre and GFP coexpression CAG Retroviral Gage 49054 CAG-GFP/cre Cre-GFP fusion CAG...116879 CAG-Cremyc-2A-GFP GFP and Cre CAG Mammalian Lu 117148 Hiv7CMV-Cremyc-2A-GFP GFP and Cre CMV Lentiviral...iRFP670-EFS:Cre-2A-GFP iRFP670, Cre, and GFP TRE Mammalian Jacks 68544 AAV-Cre-GFP Cre CMV AAV Nestler...13770 pCALNL-GFP Cre dependent GFP expression Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed gene switch...promoterless CRE-GFP fusion none Mammalian Sauer 11960 pBS537 tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV... CMV Lentiviral Fuchs 26646 pCAG-Cre-IRES2-GFP Cre and GFP coexpression CAG Mammalian Chenn 26647 pCAG-Cre...CAG Retroviral Gage 49056 AAV-GFP/Cre Cre-GFP fusion CMV AAV Gage 49111 pEMS1980 iCre with MCS for inserting...
  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate...The applications for such a modular system are endless and, as scientists use GMAP, there will be a continuous...ferritin protein were tethered to the C-terminus of TRPV4 (a pressure-sensitive channel). The Guler lab optimized...Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry Mammalian Expression ...
  8. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...2013 Mar 11. pET28:GFP pET28 driving expression of GFP, biobrick part Bba_E0040 GFP Bacterial Expression...doi: 10.1038/ncomms1516. pBW400hrpL-gfp pBW400hrpL-gfp hrpL-rbs30-gfp-ter (Synthetic) Bacterial Expression....0124958. eCollection 2015. MXS_PA-GFP MXS_chaining vector with PA-GFP PA-GFP (Synthetic) Synthetic Biology...Biotechnol. 2006 Oct . 72(5):1014-23. pET30-R5-GFP pET30-GFP Bacterial Expression, Synthetic Biology Keasling.... Epub 2010 Dec 28. SS13 lac-inducible GFP reporter circuit GFP Bacterial Expression, Synthetic Biology...expression. J Biol Eng. 2011 Sep 20;5:12. pBbB1a-GFP GFP (Synthetic) Bacterial Expression, Synthetic Biology...expression. J Biol Eng. 2011 Sep 20;5:12. pBbB1c-GFP GFP (Synthetic) Bacterial Expression, Synthetic Biology...
  9. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...unpublished) lenti-Cas9-VQR-GFP_activity_reporter GFP and sgRNA targeting GFP (NGA restricted) Lentiviral... doi: 10.1016/j.cell.2013.12.001. pRS316-RGR-GFP RGR-GFP (Synthetic) Bacterial Expression, Yeast Expression...Dec 30. doi: 10.1111/jipb.12152. pRS316-RGR-GFP-mHH RGR-GFP (Synthetic) Bacterial Expression, Yeast Expression...Dec 30. doi: 10.1111/jipb.12152. pRS316-RGR-GFP-mHDV RGR-GFP (Synthetic) Bacterial Expression, Yeast Expression...Dec 30. doi: 10.1111/jipb.12152. pRS316-RGR-GFP-mm RGR-GFP (Synthetic) Bacterial Expression, Yeast Expression....059477.116. doi: 10.1261/rna.059477.116. pX330-GFP-SFPQ sgRNA GFP-SFPQ (Homo sapiens) CRISPR Fox Functional...pii: 4791732. doi: 10.1093/glycob/cwx101. pX552-GFP GFP (Other) Mammalian Expression, AAV, CRISPR Hewitt...
  10. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...Jul 1. 308(1):8-15. SS13 lac-inducible GFP reporter circuit GFP Bacterial Expression, Synthetic Biology....1073/pnas.1220157110. Epub 2013 Mar 11. pET28:GFP GFP Bacterial Expression, Synthetic Biology Bennett...:508. doi: 10.1038/ncomms1516. pBW400hrpL-gfp hrpL-rbs30-gfp-ter (Synthetic) Bacterial Expression HrpL...Microbiol. 2005 Nov . 71(11):6856-62. pPro7(E)-gfp GFPuv Bacterial Expression, Synthetic Biology Keasling...enterica. Gene. 2006 Aug 1. 377():6-11. pPro24(s)-gfp GFPuv (Other) Bacterial Expression, Synthetic Biology...Environ Microbiol. 1997 May . 63(5):1689-95. pPro24-gfp GFPuv Bacterial Expression, Synthetic Biology Keasling...degradation. J Biol Eng. 2012 Jul 9;6(1):9. PRM-GFP CI inducible sfGFP Bacterial Expression, Synthetic Biology...
  11. Molecular Biology Reference

    Type
    Guide
    ...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic...AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG Tyrosine Tyr Y UAU, UAC Valine Val V GUU, GUC... constructed a plasmid, you can easily make an endless number of copies of the plasmid using bacteria,.... Visit our viral service page to learn more. Regardless of type, plasmids are generally propagated, selected...Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated, the antibiotic powder can ...
  12. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...inserted; with Cas9 + GFP pSpCas9(BB)-2A-GFP (PX458) px458_Conc3 U6 GFP Stange Universal and...of a GFP flip-out cassette pUASTattB pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP Cas9-T2A-GFP Perrimon...OTF1, Oct1Z, oct-1B pLV hUbC-Cas9-T2A-GFP humanized Cas9 T2A GFP (Other) hUbC Gersbach Multiplex CRISPR... Lenti-Cas9-blast pLentiCas9-T2A-GFP T2A-GFP In frame with Cas9 Blasticidin Guigo Scalable...human ESC/iPSC pSpCas9(BB)-2A-GFP (PX458) pCAG-eCas9-GFP-U6-gRNA eSpCas9(1.1) CAG Zou pCAG-SpCas9...conjugated with mCherry pX601 pX601-GFP GFP (Other) Kan Genome editing using CRISPR-Cas9 to...Expresses huAsCpf1-T2A-GFP and crRNA guide pcDNA3.1 pY095 huLbCpf1 (Synthetic), GFP (Synthetic) CMV...
  13. CRISPR Plasmids - Yeast

    Type
    Collection
    ...EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae...L202 in eGFP. MLM3636 eGFP L138 gRNA eGFP L138 gRNA (Other) U6 Harris A panel of eGFP reporters... pRS403 eGFP L202 gRNA eGFP L202 gRNA (Other) U6 Harris A panel of eGFP reporters for single...yeast transformation pRSII425 p415-PtrpC-Cas9-TtrpC-CYC1t humanized Cas9 (Homo sapiens) LEU2 Hong...pCM188/p414-TEF1p-Cas9-CYC1t pCAS9i_TRP GAL1 (Cas9), pSNR52 (gRNA) TRP1 Bertl Bertl Lab Plasmids (unpublished...pCM188/p414-TEF1p-Cas9-CYC1t pCAS9id_TRP GAL1 (Cas9), pSNR52 (gRNA) TRP1 Bertl Bertl Lab Plasmids (unpublished...Optimized S. pyogenes Cas9 (Homo sapiens) TEF1 promoter TRP1 Church Genome engineering in Saccharomyces cerevisiae...
  14. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...pPS808 - N-terminal GFP for yeast expression pET GFP - C-terminal GFP for...gene expression GFP Varies pBABE GFP - Retroviral gene expression pMKO.1 GFP - Retroviral ...C elegans expression GFP Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression... from the SP6 promoter pCS2 mt-GFP - Create myc-tag, GFP-tag fusions for transcription ...expression pcDNA3 GFP LIC cloning vector - C-terminal GFP for mammalian expression...expression MSCV-IRES-GFP - Mammalian retroviral gene expression with GFP selectable ...cassette pBTG (pBigT-IRES-GFP) - pBigT modified to contain IRES-GFP Hynes Lab ...
  15. Synthetic Biology - Bacterial

    Type
    Collection
    ...2013 Mar 11. pET28:GFP pET28 driving expression of GFP, biobrick part Bba_E0040 GFP Bacterial Expression...doi: 10.1038/ncomms1516. pBW400hrpL-gfp pBW400hrpL-gfp hrpL-rbs30-gfp-ter (Synthetic) Bacterial Expression...Biotechnol. 2006 Aug . 24(8):1027-32. pET30-R5-GFP pET30-GFP Bacterial Expression, Synthetic Biology Keasling.... Epub 2010 Dec 28. SS13 lac-inducible GFP reporter circuit GFP Bacterial Expression, Synthetic Biology...expression. J Biol Eng. 2011 Sep 20;5:12. pBbB1a-GFP GFP (Synthetic) Bacterial Expression, Synthetic Biology...expression. J Biol Eng. 2011 Sep 20;5:12. pBbB1c-GFP GFP (Synthetic) Bacterial Expression, Synthetic Biology...expression. J Biol Eng. 2011 Sep 20;5:12. pBbB1k-GFP GFP (Synthetic) Bacterial Expression, Synthetic Biology...
  16. Plasmids for Optogenetics Research

    Type
    Collection
    ...Fubi-ChR2-GFP Excitatory ChR2 Lentiviral GFP Boyden 22217 FCK-Arch-GFP Inhibitory Arch Lentiviral GFP Boyden...AAV-FLEX-Arch-GFP Inhibitory Arch AAV GFP Boyden 22223 FCK-Mac-GFP Inhibitory Mac Lentiviral GFP Boyden 22224...pAAV-FLEX-ArchT-GFP Inhibitory ArchT AAV GFP Boyden 29777 pAAV-CAG-ArchT-GFP Inhibitory ArchT AAV GFP Boyden 29778...pAAV-CamKII-ArchT-GFP Inhibitory ArchT AAV GFP Boyden 37810 pAAV-CAG-Arch-GFP Inhibitory Arch AAV GFP Boyden 41015...pAAV-CAG-FLEX-Mac-GFP Inhibitory Mac AAV GFP Boyden 58805 pAAV-CaMKII-Chronos-GFP Excitatory Chronos AAV GFP Boyden...pAAV-Syn-ChrimsonR-GFP Excitatory ChrimsonR AAV GFP Boyden 58841 pAAV-Syn-CsChR-GFP Excitatory CsChR AAV GFP Boyden...pAAV-CaMKII-Mac-GFP Inhibitory Mac AAV GFP Boyden 58851 pAAV-EF1a-FLEX-ArchT-GFP Inhibitory ArchT AAV GFP Boyden...
  17. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Sep 30. expression of FnCas9-GFP in bacterial cells pET-His6-GFP-TEV-LIC pKM197 Upstream & Downstream... mutation p15A pDS05 wtCas9 (Other), gfp Veening Harnessing CRISPR-Cas9 for genome editing... sgRNA which has its spacer sequence replaced by gfp gene pcdf1-b pJV53-Cas12a FnCpf1 (Other...annotated Genbank file. DVA pD2-dcas9-J23101-GFP dCas9 (Synthetic) Poh Regulating exopolysaccharide....1038/s41598-018-31161-7. Constitutive dCas9 and GFP expression plasmid pBbA2c pFD116 Ptet...generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat. ...sgR pKD46 pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis (Other...
Showing: 1 - 17 of 17 results