Cpf1: A New Tool for CRISPR Genome Editing
Type
Blog Post
... our CRISPR guide for a refresher on CRISPR/Cas9.
How was Cpf1 found and tested?
Class 2 CRISPR systems...Addgene
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View Other CRISPR Blog Posts...over Cas9
Type II CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest...discovery of this type V CRISPR system proved we had a lot more to learn about CRISPR biology. Later work,...called Cas12a.
In 2015, Zetsche et al. added to the CRISPR toolbox with their characterization of two Cpf1...systems. A putative new class 2 nuclease, Cpf1 (CRISPR from Prevotella and Francisella), was annotated...in several genomes and is classified as a type V CRISPR system. Like Cas9, Cpf1 contains a RuvC-like endonuclease...