We narrowed to 23 results for: lacZ

Learn a variety of techniques you can use increase the fidelity of multi-part Golden Gate Assembly reactions.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

Learn more about the common E. coli strains used in the lab.

Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective expression in the brain.

Choose the best empty backbone for your experiment from some of the most popular in Addgene's library.

Learn all about how to use a new plasmid for RNAi in C. elegans, tools for proximity labeling, RNA-based methods for detecting viral outbreaks, and tools for characterizing protein domain binding specificities.

CRISPR Cas9 gRNA plasmids from the Zhang lab

Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core

Learn how to use the dTAG system to inducibly degrade your protein of interest.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Learn more about E. coli strains used for protein overexpression.

Find a list of universal sequencing primers and other common sequencing primers.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more.

Plamids for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish, plants, and human cells

Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use in C. elegan research.

List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE plasmids.

Find bacterial expression plasmids that can be used for protein purification, protein visualization, study of protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria.