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Showing: 21 - 40 of 47 results
  1. Working with Nuclear Receptors

    Type
    Blog Post
    ...evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely used today.  ...
  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly luciferase. The bait and prey...immunoprecipitated from the cells using the Protein A tag. The luciferase activities can then be measured and normalized... assess interactions. Higher relative firefly luciferase activity in the Co-IP compared of a control Co-IP...
  3. Bacterial Expression Systems

    Type
    Collection
    ...pTac GFP, Luciferase (LuxCDABE) Attila Karsi Broad host range vectors for expressing luciferase and fluorescent...Quorum sensing molecule) Cynthia Collins Contains luciferase inducible by the quorum sensing molecule, 3OC6HSL...promoter can be found in pCS-PesaRlux controlling luciferase expression. pCW-LIC 26098 3x Tac promoter (hybrid...
  4. Fluorescent Protein Guide

    Type
    Collection
    ... Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I Use? Blog: How...
  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected with the test ...
  6. Sequencing Primers

    Type
    Guide
    ...AGTCAAGTAACAACCGCGA 3' end of luciferase, forward primer LucNrev CCTTATGCAGTTGCTCTCC 5' end of luciferase, reverse primer... primer LucNrev CCTTATGCAGTTGCTCTCC 5' end of luciferase, reverse primer M13 Reverse CAGGAAACAGCTATGAC...Rluc-F CCAGGATTCTTTTCCAATGC 3' end of Renilla luciferase, forward primer RVprimer3 CTAGCAAAATAGGCTGTCCC...
  7. Plasmid Collections

    Type
    Collection
    ...Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus...
  8. Tetracycline Inducible Expression

    Type
    Collection
    ...single copy in mammalian cells; Expresses firefly luciferase hairpin and GFP under pTREtight promoter None...pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site; see article for additional selection...
  9. Cre-lox system

    Type
    Collection
    ...backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Cre, luciferase, and sgRNA expression EFS AAV Zhang 60229 AAV...Retroviral Luikart 67503 pF CAG luc-EGFP-cre puro Luciferase - EGFP- Cre fusion CAG Mammalian Stringer 68448...12463 p231 pCMVe-betaAc-STOP-luc Cre dependent luciferase expression Mammalian Green 32145 pJFRC172-10XUAS-loxP...
Showing: 21 - 40 of 47 results