Antibodies 101: Polyclonal Antibodies
Type
Blog Post
...bind the IgG heavy chain. Running the serum over a column of resin crosslinked to protein A /G will bind ... antigen. In this case, the serum is run over a column containing the target protein, and any antibody...recognizing the target protein remains bound to the column while the rest of the proteins are eluted. Then...Then, the antibodies are dissociated from the column and from the target protein.
Pre-adsorption
Finally... pre-adsorb the antibodies, pass them through a column containing immobilized serum protein from other...-react with the serum proteins pass through the column, resulting in a polyclonal antibody population ...