...minor 475 nm peak, respectively. When wtGFP is illuminated with UV-violet light (~400 nm), the chromophore...PA-GFP to display almost no fluorescence when illuminated with 488nm light. However, when UV-violet light... to a 100-fold increase in fluorescence when illuminated with blue light (1). Similar to PA-GFP, scientists...derived from DsRed and mRFP, respectively. When illuminated with violet light, both variants are photoconverted...

... the sequence of interest. 5. Use a silica spin column for purification of the samples you send for DNA...isopropanol precipitation method or using a silica spin column available from several vendors. The precipitation... I would strongly recommend using a silica spin column. They can be pricey, but well worth it. 6. Edit...

...descending order, in the case of numeric columns.  The data in any column are searchable and you can search ...You can access the datatable here. Current table columns are target gene, species, sequence, Addgene plasmid...

... sharing concerns. Ask alumni of the lab. Most labs have websites listing alumi and it is easy to use ...ignore warning signs If lab members, neighbors or alumni give you information about the PI that concerns...

...contributed by Theresa Liao of the University of British Columbia. When transitioning from an academic science career...Good luck! Resume 101 from University of British Columbia, Student Services - includes videos on general... of Physics & Astronomy, University of British Columbia. Originally on track for a research career, she...

...locally. Your university probably has an alumni directory. Alumni who enter their information in these searchable...information so you can tap them for advice. As a future alum, these are people you can “cold call” and ask for...

...plexWell technology to easily and quickly create Illumina sequencing libraries. From start to finish the...libraries are then QC’d, pooled, and prepared for Illumina sequencing on our newly donated MiSeq.  For Complete... also adds the necessary adapter sequences for Illumina sequencing as well as unique barcodes per sample...

...saturation density, decreased rates of growth, or clumping. To ensure the best possible results for any tissue...approach is that it tends to result in a higher volume of lower titer virus. In such cases you may want...them! Changes in such variables as cell density, volume, incubation time, and MOI can all impact the success...

...what if an FP fusion with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm...control of secretory proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik is also... inheritance independently of the endoplasmic reticulum." The Journal of cell biology 154.2 (2001): 317...

... full plasmid sequencing at our facility using Illumina MiSeq Next Generation Sequencing (NGS) technology...need to modernize our approach to handle larger volumes of data. In this two-part blog series, we will ...continuous updates. The generous donation of a Illumina MiSeq sequencer enabled us to perform the necessary...

...barcoded, locus-specific libraries compatible with Illumina next generation sequencing in order to study evolutionary..., with each plasmid carrying a unique barcoded Illumina-M13F bridging primer. These bridging primers are... are amplified and used to generate Illumina sequencing libraries using a 2-step PCR-based protocol and...

...mechanisms that generate luminescence. This can then be detected by a luminometer. Non-specific DNA stains...sensitivity but low specificity (Degeling, 2012). Bioluminescence assay kits are available from different biotech...

...and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...  Tips for flow cytometry success Break up cell clumps Success in a flow cytometry experiment depends ... through a nylon mesh cell strainer to break up clumps before running on the instrument. To sustain cell...

...injections are administered in the cisterna magna or lumbar spine. Image and caption courtesy of Challis et...parentheses in the"Mouse Strain-Specific Tropism?" column. For a complete list of mice strains with LY6A-...transducing the macaque brain, except for the cerebellum, brain stem, and spinal cord. All PALs, including...compared to AAV9 in the cerebrum, but not the cerebellum, in macaques. It also effectively transduces ...

...MEP CTSS cathepsin S MGC3886 ERAP1 endoplasmic reticulum aminopeptidase 1 A-LAP, ALAP, APPILS, ARTS-1, ..., KIAA0525, PILS-AP, PILSAP ERAP2 endoplasmic reticulum aminopeptidase 2 FLJ23633, FLJ23701, FLJ23807,...

...using an iodixanol column gradient. We'll show the loading and unloading of the column and give some tips...

...each plasmid needed for the whole experiment Total volume (μL) of plasmid needed for the whole experiment... the equation is labeled with its corresponding column in the calculator spreadsheet: We hope that this...

...Red2Green, a program that uses this waste as plastic lumber for things like truck bumpers, plastic adirondack...this material to produce various grades of plastic lumber and distribute it to industrial and residential...

...a CRISPR array in the resulting Ignavibacteriae phylum genome. IgnaviCas9 is a hyperthermostable Cas9 ...IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans, whose optimal growth temperature...

... require injection of substrate to produce bioluminescence. Substrate pharmacokinetics and biodistribution...localization: In vivo imaging with fluorescent or bioluminescent reporters usually only detects the signal of...