Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Showing: 61 - 80 of 228 results
  1. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...plexWell technology to easily and quickly create Illumina sequencing libraries. From start to finish the...libraries are then QC’d, pooled, and prepared for Illumina sequencing on our newly donated MiSeq.  For Complete... also adds the necessary adapter sequences for Illumina sequencing as well as unique barcodes per sample...
  2. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...what if an FP fusion with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm...control of secretory proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik is also... inheritance independently of the endoplasmic reticulum." The Journal of cell biology 154.2 (2001): 317...
  3. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...saturation density, decreased rates of growth, or clumping. To ensure the best possible results for any tissue...approach is that it tends to result in a higher volume of lower titer virus. In such cases you may want...them! Changes in such variables as cell density, volume, incubation time, and MOI can all impact the success...
  4. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...barcoded, locus-specific libraries compatible with Illumina next generation sequencing in order to study evolutionary..., with each plasmid carrying a unique barcoded Illumina-M13F bridging primer. These bridging primers are... are amplified and used to generate Illumina sequencing libraries using a 2-step PCR-based protocol and...
  5. Switch to GECO? An Overview of AAV Encoded Calcium Sensors

    Type
    Blog Post
    ...jGCaMP6, but do not show photoswitching after illumination with blue light (as jRGECO1 does). Since some...optogenetic tools (e.g., Channelrhodopsin, ChR2) are illuminated by blue light, the stability of jRCaMP1a, b to...R-GECO and R-CaMP2, exhibit photoswitching when illuminated with blue light, causing a transient increase...
  6. Analyzing Single Cells with Flow Cytometry

    Type
    Blog Post
    ...and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...  Tips for flow cytometry success Break up cell clumps Success in a flow cytometry experiment depends ... through a nylon mesh cell strainer to break up clumps before running on the instrument. To sustain cell...
  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ...MEP CTSS cathepsin S MGC3886 ERAP1 endoplasmic reticulum aminopeptidase 1 A-LAP, ALAP, APPILS, ARTS-1, ..., KIAA0525, PILS-AP, PILSAP ERAP2 endoplasmic reticulum aminopeptidase 2 FLJ23633, FLJ23701, FLJ23807,...
  8. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... a thermostable Cas9 variant Scientific Reports volume 11, Article number: 9586 (2021) Expresses a thermostable...pACYC184 pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710 Zhang RNA targeting with CRISPR-Cas13...
  9. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...each plasmid needed for the whole experiment Total volume (μL) of plasmid needed for the whole experiment... the equation is labeled with its corresponding column in the calculator spreadsheet: We hope that this...
  10. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...Red2Green, a program that uses this waste as plastic lumber for things like truck bumpers, plastic adirondack...this material to produce various grades of plastic lumber and distribute it to industrial and residential...
  11. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ... may be electrochemical or light-based, with luminescent and fluorescent reporters being especially popular...such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes. Examples ...
  12. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... The amount of aeration, temperature, culture volume, antibiotic, and medium can all affect copy number...conditions aren't working against you!  The culture inoculum Freshly streaked bacteria have higher copy numbers...
Showing: 61 - 80 of 228 results