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Showing: 81 - 100 of 213 results
  1. Plasmids 101: Protein tags

    Type
    Blog Post
    ...metal-affinity chromatography, usually using a Ni2+ column. Like other affinity tags, a His tag can be fused...with the cleavage reaction taking place between column runs. The TEV protease itself, with various mutations...
  2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...oviducts. After solution injection into the oviductal lumen, tweezer-type electrodes are placed to mediate electroporation...i-GONAD is that it requires a high-concentration, column-purified DNA template.   Many thanks to Channabasavaiah...
  3. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...dark areas (protected) as opposed to brightly illuminated ones (risky). They also prefer to explore novel...References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability...
  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...recently developed a new method called DULIP (dual luminescence-based co-immunoprecipitation) to overcome the... this kit are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being used for... pooled library Parasites from the apicomplexan phylum cause severe human and livestock diseases such ...
  5. Finding Your Science Policy Path

    Type
    Blog Post
    ...so don’t be shy and reach out! Someone from your alumni community is a great place to start. What is science.... Many fellowship programs can connect you with alumni before and during your application process so you...
  6. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...visualize the protein via fluorescence or chemiluminescence, precipitate the protein out of solution, ...antibody allows the protein to be detected by chemiluminescence or fluorescence. Western blots are frequently...
Showing: 81 - 100 of 213 results