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Showing: 101 - 120 of 257 results
  1. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp., Bordetella spp...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...
  2. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...dark areas (protected) as opposed to brightly illuminated ones (risky). They also prefer to explore novel...References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability...
  3. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...oviducts. After solution injection into the oviductal lumen, tweezer-type electrodes are placed to mediate electroporation...i-GONAD is that it requires a high-concentration, column-purified DNA template.   Many thanks to Channabasavaiah...
  4. CRISPR 101: Validating Your Genome Edit

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    Blog Post
    ...trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093...CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093...
  5. Plasmids 101: Dimers and Multimers

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    Blog Post
    ... sequencing, (which differ from the short-read Illumina MiSeq sequencing that Addgene uses.) However, ...article.   Long-read sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid sequencing ...
  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...sensitivity, and photostability under two-photon illumination than previous GEVIs. Some constructs are also...00916-3. doi: 10.1016/j.cell.2022.07.013 pYtags illuminate RTK signaling Receptor tyrosine kinases (RTKs...
  7. 27 Hot Plasmids from 2016

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    Blog Post
    ...recently developed a new method called DULIP (dual luminescence-based co-immunoprecipitation) to overcome the... this kit are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being used for... pooled library Parasites from the apicomplexan phylum cause severe human and livestock diseases such ...
  8. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ...(Erdmann et al., 2019). Check out our recent Illuminating Choices blog post for more discussion about ...from Addgene Better Dyeing Through Chemistry Illuminating Choices: A Guide to Selecting Fluorescent Dyes...
  9. Deep Dive: Statistical Tests (Comparisons)

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    Blog Post
    ... humidity, sunlight exposure time, and watering volume on total crop production (though you would not ...Carlo Simulation Study. Front. Appl. Math. Stat. Volume 2 (2016). doi.org/10.3389/fams.2016.00002  ...
  10. Finding Your Science Policy Path

    Type
    Blog Post
    ...so don’t be shy and reach out! Someone from your alumni community is a great place to start. What is science.... Many fellowship programs can connect you with alumni before and during your application process so you...
  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...visualize the protein via fluorescence or chemiluminescence, precipitate the protein out of solution, ...antibody allows the protein to be detected by chemiluminescence or fluorescence. Western blots are frequently...
Showing: 101 - 120 of 257 results