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Showing: 61 - 80 of 708 results
  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ...tagging of proteins expressed from their natural chromosomal context. These CRISPR tagging methods allow for...
  2. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGCTTTCACGCATTGCACtacacattggcaagatgGCAGCCACCATCGGGAGA prothymosin alpha a TAL3344 & TAL3345 TTATCATTCGCATCTCGTatttctctttatattaTTTTATTCCGAGACCCCA...
  3. Zhang Lab CRISPR Page

    Type
    Collection
    ... (SpCas9) nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single...
  4. Luciferase Plasmids

    Type
    Collection
    ...gene in vitro and in vivo to use in biosensors. The most common types of luciferase found in Addgene’s collection...
  5. Validated gRNA Sequences

    Type
    Collection
    ...GAGTCCGAGCAGAAGAAGAA 69236 cut S. pyogenes 26480473 Wolfe ttTi5605 Mos1 insertion site C. elegans ATATCAGTCTGTTTCGTAA 47550...
  6. CRISPR Library Amplification

    Type
    Protocol
    ...than four Maxipreps worth. Count colonies on the most dilute Petri dish. Total colony yield = count x ...in library. Example: At least 10 colonies on the most diluted plate for 1000x coverage of a library of... are present. If colonies are not present on the most dilute plate, count the second plate (ie. the 1:...tend to report lower values than Nanodrop, although most protocols have been designed with Nanodrop as the...
  7. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...number of cells is limited (e.g. primary cells). Most CRISPR libraries require the use of lentivirus to...
  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti-...so commonly used is that it is good at infecting most cells, but not all. For example, LDLR expression...envelope proteins for pseudotyping While VSV-G is the most commonly used envelope protein for pseudotyping,...
  9. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...Susanna Harris, Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg, Heather Martinez, Pat Schloss...don’t have the networks or experience to get the most of meeting.  I experienced this at the ASM General...and helping societies help their members get the most out of their meetings. Amanda Gunn ran randomized...
  10. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...been generated by restriction enzyme digestion . Most restriction enzymes digest DNA asymmetrically across...size of each and their concentration. However, for most standard cloning (where the insert is smaller than... + Background due to vector re-circularization - most useful for phosphatase treated vector Insert or ...
  11. Using a Light Microscope Protocol

    Type
    Protocol
    ...so, for this protocol, we'll focus on one of the most common types that you're likely to encounter: the...properties of light to detect small objects. Two of the most important properties of a microscope are magnification... will see several pieces of information, but the most important (for now) is the magnification power, ...
  12. Cre-lox system

    Type
    Collection
    ...pMAZe Reporter construct for lineage tracing and mosaic analysis in zebrafish Zebrafish Lewis Additional...
  13. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...gently spread over the surface until the liquid is mostly absorbed (there is a very small visible volume ...gently spread over the surface until the liquid is mostly absorbed. The spreading of cells can be done in...
  14. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ... following amendments: Note: Gel purification is most efficient with lower % agarose gels, so you will...will want to isolate the DNA from the gel. This is most commonly done with a commercial gel purification...
  15. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...they recognize, restriction digests have become the most widely used method scientists employ to selectively...cut the plasmid into 3-8 pieces such that all (or most) fragments are small enough to be accurately sized...
  16. Centrifugation

    Type
    Protocol
    ...labels for the core parts that would be found on almost any centrifuge. Types and Parts There are several...techniques. Regardless of the type of centrifuge you use, most will have essentially the same components. The photo...
  17. Gibson Assembly Protocol

    Type
    Protocol
    ...Cloning? No need for specific restriction sites. Join almost any two fragments regardless of sequence. No scar...contiguous DNA fragment. *Pro-Tip* Add Extreme Thermostable Single-Stranded DNA-Binding protein (ET SSB)...
  18. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...contains the plasmid DNA separated from bacterial chromosomes. Collect the supernatant into a new tube by pipetting.... This is included in the resuspension buffer of most kits. Reference Page | Top | Index...
Showing: 61 - 80 of 708 results