...Structure Plasmids Phamret 458 517 14 Monomer Phamret-N1 - Mammalian Expression Phamret-C1 - Mammalian Expression...Empty Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson...- Mammalian Expression pMXs-mRFP1 - Mammalian Retroviral Expression mCherry 587 610 16 4.5 15 min Monomer...

...plasmids third-generation rtTA On Elledge 19407 pTREtight2 Promoter contains a modified TRE that is silent...Expresses firefly luciferase hairpin and GFP under pTREtight promoter None Either Elledge 35625 pAAV-Ptet-RFP-shR-rtTA... 3rd generation rtTA On Campeau 25434 pMA2640 Retroviral; CMV-driven; linked via IRES to EGFP-Blasticidin...EGFP-Blasticidin fusion; pMA2641 has rtTA driven by retroviral LTR promoters ; see article for lentiviral vectors...

...materials are available only in the US at this time. Return to top Plasmids Encoding Mammalian Genes or Inserts...related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools SHERLOCK and DETECTR...Transcriptase plasmids from Andrew Ellington's lab. High-surety isothermal amplification and detection of SARS-...lab to ask them to deposit the plasmid with us. Return to top Pooled Libraries SARS-CoV-2 Spike (S) Ectodomain...

...Browse all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...

...antibodies to zebrafish cell surface receptors and secreted proteins....antibodies to zebrafish cell surface receptors and secreted proteins. The plasmids encode both the light and...transfecting mammalian cell lines and collecting the secreted monoclonal antibody from the supernatant. Please...

...serotypes AAV1, 2, 5, 8, 9 and retrograde Engineered Serotypes Retrograde AAV to target projection neurons...Serotype). Convert a Penn Catalog ID to an Addgene ID Return to top Viral Service Suggestions Don’t see what...

...developing mouse retina. To aide in their studies of the regulatory networks involved in retinal development...plasmids to study the regulatory networks involved in retinal development. Depositor...

...and HNH. Cas9 nickase, a D10A mutant of SpCas9, retains one nuclease domain and generates a DNA nick rather...editors carrying the delta-984-1090 ADAR truncation retain RNA editing capabilities and are small enough to...that cannot cleave target DNA. The dCas9 molecule retains the ability to bind to target DNA based on the ...AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells that are poorly...homology-dependent repair. 2018. Charpentier M, Khedher AHY, Menoret S, Brion A, Lamribet K, Dardillac E, Boix C, Perrouault... FJ, Terns RM, Terns MP, White MF, Yakunin AF, Garrett RA, van der Oost J, Backofen R, Koonin EV. Nat ...Cas systems. 2013. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ...

...extracellular regions of cell surface receptor and secreted proteins as soluble recombinant molecules....extracellular regions of cell surface receptor and secreted proteins as soluble recombinant molecules. The...proteins P. falciparum sporozoite cell surface and secreted proteins Mouse sperm Mouse egg Laboratories using...

...Plasmids RXRA, NR2B1 retinoid X receptor, alpha RXRG Plasmids RXRG, NR2B3, RXRC retinoid X receptor, gamma... receptor gamma RARA Plasmids RARA, NR1B1, RAR retinoic acid receptor, alpha RORA Plasmids RORA, ROR1,...

...81070 rAAV2-retro helper AAV retrograde AAV packaging plasmid, expressing Rep2 and retrograde capsid Karpova...

...was generated with the help of David Sabatini . Return to top mTOR Pathway - Gene List Click on a name...sclerosis VHL Von Hippel-Lindau tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2...complex 2 SGK Serum/glucocorticoid regulated kinase 1 Return to top Resources Cancer Pathway ORF Kit (Sabatini...

...AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406 SMC1A-mEGFP AICSDP-57 mEGFP ...SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus...

... AM Cre and Flpo Katherine Matho 10:15 AM AAV Retrograde Tracing Gowan Tervo and Sarada Viswanathan 11...of research materials to share: In vivo data: Retrograde hSyn1-GCaMP6f-P2A-nls-dTomato Detailed protocol....doi: 10.3389/fnana.2019.00093. PubMed 31849618 Return to top...

..., or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits are sets...synthetic biology plasmids for use in C. elegans . Return to top Other Resources Addgene Blog Posts An “elegans...genome of the genetic model organism C. elegans. Return to top Do you have suggestions for other plasmids...

...Frederick National Laboratory for Cancer Research Return to top Ras Pathway - Gene List Click on a name ...RASSF10 Ras association domain family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 ...exchange factor 1 YAP1 Yes associated protein 1 Return to top Resources RAS Pathway Clone Collection 2.0...

... Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and useful types...MSCs. Use packaging construct encoding Tat. Reiser Return to Top Popular Lentiviral Transfer Plasmids The...article for more versions of this plasmid. Root Return to Top Do you have suggestions for other plasmids...

...powerful the FRET signal. In well-designed FRET-based biosensors, the intensity of the FRET signal can ...packaged into a retrovirus for infection of host cells. The fluorescent moiety of the BRET reporter was ...proteins together thereby increasing their FRET signal. FRET is a non-radiative transfer of energy from...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase... allows researchers to convert FRET biosensors into more versatile BRET biosensors that can even be paired...PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein... on the Addgene Blog Learn more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent...

...developed a new AAV variant, rAAV2-retro, that migrates retrogradely at higher rates than any other AAV... AAV2-retro vectors with a variety of transgene/promoter combinations. Check out our retrograde AAV blog...Selective retrograde transsynaptic transfer of a protein, tetanus toxin, subsequent to its retrograde axonal...strengthened during memory formation and memory retrieval. In neuronal tracing experiments, brain tissue...bodies to the terminals of their axons; while retrograde tracing outlines neurons in the opposite direction...axons to their cell bodies. Anterograde and retrograde tracing take advantage of existing transport ...myosin, and enzymes for transmitter synthesis. Retrograde transport is used for transport of endocytosed...

...TGTTAAGGCTCTGTATAGcagtgtgtgtcctggcCACTTGCTGGGCTCAGGA retinitis pigmentosa 2 (X-linked recessive) TAL3526 & TAL3527...TCTTTTTGTGCTGCGCCAcccagcccataatcgaGTCTTCTACAGGCATGAA retinitis pigmentosa GTPase regulator b TAL3528 & TAL3529...TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA retinitis pigmentosa GTPase regulator b ORF15 TAL3530 & ...