...heat-shock Shorten or skip the outgrowth (for Ampicillin resistance it is ok to completely skip the outgrowth,...plasmid) you can save a lot of time by shortening or skipping many steps and will still get enough colonies ...

...toolkit for human gene therapy. Wu Z, Asokan A, Samulski RJ. Mol. Ther. 2006 Sep;14(3):316-27. Epub 2006...independently of DNA synthesis. McCarty DM, Monahan PE, Samulski RJ. Gene Ther. 2001 Aug;8(16):1248-54. PubMed ...

...careers divided by the most pertinent skills required Research skills The ability to think critically and...valuable skills acquired from a science degree that can be applied to many roles. These types of skills show...and formatting skills, which are useful to many employers. Increasingly, computer skills are highly desired...and good computer skills will give you an advantage. Strong writing, computer skills (Word, Excel, PowerPoint...desirable quality sought after by employers. These skills show employers that you can fully investigate and...There are many roles that actively require these skills, including: Science Reporter Scientific Sales...your tasks done correctly the first time. These skills are very desirable to employers because they save...

...knowledge and skills outside of the lab Effective verbal and written communication skills are key to publication...career path and identifying needed skills First, evaluate your skills, values, and interests. Are you particularly...professional skill sets are strengthened. This short list highlights the ubiquity of skills across professions...to students to identify and build the necessary skills for alternative careers. Science Careers has developed...a variety of science professions based on their skills and interests. Once scientists identify potential...potential career pathways, education and mastering new skill sets must occur by doing work outside of the lab...careers outside of the lab highlight “transferable” skills such as leadership, management, and communication...

...Concepcion CP, Bonetti C, Vidigal JA, Han YC, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe...

...information. Think "skills," not just "research and publications” What are the skills you developed through...candidates. Write about your transferable skills Some of the skills you developed might not have come from...can develop more transferable skills? See Developing Transferable Skills During Science Training and So...interested in. Consider listing the skills you already have, and the skills you need and don't have yet for...candidate for the job. How can you incorporate the skills developed during graduate school into your resume...functional one. Pick one that makes sense for your skills, and the job you are applying for. Also remember...write about the research you did. Emphasize the skills. If you studied astronomy or bioinformatics data...

...expertise, professional skills, relevant extracurricular activities, transferable skills, and accomplishments...communication skills” (a common requirement for many positions), it is better to support your skills or achievements...face it, cover letters are read and resumes are skimmed.” So if you really want to be the best candidate...principle: they have to be original and show that your skills, experience, and personality really fit what the... department and the institution, the particular skills required, and the expectations for teaching and...research], and have excellent [list the most important skills for the position]. I am enthusiastic about [something...the job]. I believe that, with my experience and skills, I will make a great addition to your company and...

...forms of dystrophin can still be functional, exon skipping is a good option for DMD treatment. Clinical trials... trials have used oligonucleotide exon skipping (OEN) to remove mutated exons from the dystrophin transcript... to explore genome editing approaches for exon skipping. Addgene depositor Charles Gersbach used paired... Could this approach work with dystrophin exon skipping? For such a therapy to be successful, multiple...downregulated in mature tissues - no problem, as exon skipping proceeds through the non-homologous end joining...difference in DMD, and it’s estimated that exon skipping therapies would be applicable to 80% of DMD patients... and Tabebordbar et al. each chose to try exon skipping in DMD mice, which have a mutation in dystrophin...

...evolve your transferable skills. Transferable skills are the non-technical skills you pick up wherever you...in the lab whether you realize it or not. These skills might include collaborating, managing, and communicating...communicating. After years of focusing on my technical skills with cloning and lentivirus production, I didn’... start to recognize my transferable (or “soft”) skills until I was out of the lab and in an office environment...environment. Ultimately, it will likely be these skills that get you your next job or promotion. Also, ... about these--get started with our Transferable Skills Guide.     Over the last decade, my peers and...Visit our careers topic page Read our transferable skills guide Read blog posts about career readiness Resources...

...from good management? Aren't all career skills leaderhsip skills when done well? Do you just have to know...urged you to work on developing your “leadership skills”. Leadership is prized at all levels of an organization...often asked to give career seminars on Leadership Skills. After attempting to put together such a presentation...many times, I could never actually figure out what skills were really leader-specific. How is leadership ...understanding what is expected of them and learning new skills to get the work done efficiently and with high ...be doers for a long time before they master the skills they need to be successful. Scientists are lifelong...unidentified issues. There are many good resources on the skills and characteristics of successful leaders. Below...

...list of topics/skills you are interested in exploring. Mentor:  Make a list of topics/skills in which you...temptation to skip the gym is strong. There are a few women that I see at the gym every day. If I skip, I get... Develop coaching skills Encourage your creative thinking Enhance leadership skills Support inclusion,...chatting stage you will rarely make any change in your skill sets or activate the helpful accountability that...get a bunch of text messages asking me, “What happened? Where are you?” My gym posse holds me accountable...

...interpersonal and communication skills. Problem solving and analytical skills are prerequisites but these ... in technical support make excellent use of the skills developed by life science PhDs. In my days as junior...Online live chat is a convenient alternative for asking a quick question or making initial contact, but...right place to live it out, but ideally without asking too many (annoying) questions. Identifying and ...A job in technical support can add a variety of skills to your CV and lead to other job opportunities ...understanding of when to use these communication skills by working with people from many different backgrounds...not work for everyone. The benefit is that these skills are highly transferable and will help you enter...

...attention you need? Are you good at asking for help? If you are good at asking for and getting help, size might...you want to know and display character traits and skills that you hope to grow.  Find mentors who take their...diversity in all forms? How are their organizational skills? Are they involved with colleagues and the department...    Will you be encouraged to develop transferable skills by attending career events or being involved in...has no idea who is dating whom Expanding your skillset Scientists are lifelong learners. I believe we...have access to information from other labs with skills that you can learn? Do lab members work on more...on the Addgene blog Check out our transferable skills guide Learn about careers in science communication...

...cell and 2. prevent reinfection of those cells. Kaminski et al. designed gRNAs to target the HIV-1 5’ and...Importantly, no off-target effects were detected. Kaminski et al. observed no negative effects on cell viability...up to 7 mismatches to the gRNA target sequence. Kaminski et al. next tested their procedure in HIV-1 infected...the odds of developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed...References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K,...plasmids used in this publication at Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang.... PubMed Central PMCID: PMC4649911. Khalili K, Kaminski R, Gordon J, Cosentino L, Hu W. Genome editing...

...are rarely chosen because they have demonstrated skill at managing and this is especially true in science...good manager. The kind of smarts and the type of skills that it takes to be a good scientist are not the...on Emotional Intelligence or to hone delegation skills, for example. So aside from a focus on removing...an open mind to be willing to learn and grow in skills. Managing a team is hard and most people have some...do before they can be good at it. Like any other skill, it can be developed with education and practice...introductory reference books on managing people: Skills for New Managers by Morey Stettner Successful Manager's...

...at the University of Alberta studying with the Szymanski lab. Reasons to study how a phage recognizes its...useful and cost-effective method that our lab (the Szymanski lab, which has recently moved from the University...a technique published by Simpson, Sacher, and Szymanski in January 2016 in Viruses (5). Please refer to...protein! First, block your membranes with BSA or skim milk as you would for a Western blot in order to...at the University of Alberta studying with the Szymanski lab (though she’s tagged along with the lab during...David J., Jessica C. Sacher, and Christine M. Szymanski. "Exploring the interactions between bacteriophage-encoded...David J., Jessica C. Sacher, and Christine M. Szymanski. "Development of an Assay for the Identification...

...all build and develop skill in science communication over time.  Like most skills, great science communication...that communication is the single most important skill that scientists need to succeed in their work. While...theory. So, what can you do to ensure that your skills in science communication will be a positive asset...practice. You can bootstrap your science communication skills with training programs, which are increasingly ...of this as a jumpstart or a refresher for your skillset rather than a permanent fix. Treat every interaction...

...develop a protocol that skipped the ultracentrifugation altogether. Chomczynski and Sacchi showed that ...that are found in and on everything, including your skin. Here are some general tips for working with RNA...this no-kit method are still widely used today (Chomcynski and Sacchi, 2006).  View our protocol for RNA...5294–5299. https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi N (1987) Single-Step Method of RNA ...https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method of ...

...to develop the manual skills at the regular microscope, and the scripting skills at the automated microscope...and our research goals in focus. Developing these skills relies upon biologists and computer scientists ...but don’t really know how to start applying their skills to it. The Kittybiome project gives them access...traditional biology lab, we have members with a wealth of skills to help do science on the cheap using equipment...obsolete amazingly fast, but with a wide range of skills among our members we’ve managed to learn a lot ...

...occurring E. coli plasmid ColE1 (Goebel & Helinski, 1968; Bazaral & Helinski, 1968), in which the plasmid was ..., taking many forms (Levene, 2009; Higgins & Vologodskii, 2016). Plasmid multimerization occurs via homologous... PMID: 21285356; PMCID: PMC3060453. Bazaral M, Helinski DR. Characterization of multiple circular DNA ...doi: 10.1038/294184a0. PMID: 7029308. Goebel W, Helinski DR. Generation of higher multiple circular DNA...PMID: 4884687; PMCID: PMC225270. Higgins NP, Vologodskii AV. Topological Behavior of Plasmid DNA. Microbiol...