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Showing: 41 - 60 of 89 results

  1. CRISPR-Cas/RGN expression plasmids for Zebrafish

    Type
    Collection
    ... expression plasmids for Zebrafish... CRISPR Joung Lab CRISPR Plasmids Zebrafish CRISPR-Cas/RGN expression...expression plasmids for Zebrafish You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR...CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology in zebrafish. The Joung Lab has demonstrated the use of these...modify nine different target sites in endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013...producing mRNA suitable for expression of Cas9 in zebrafish can be found in Hwang & Fu et al., Nat Biotechnol...

  2. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...arrays targeted to various zebrafish genes. The table below lists zebrafish genes and associated pairs...A list of zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs... Engineered Zinc Finger Arrays for Endogenous Zebrafish Gene Targets Recent published reports have shown...effective tools for making targeted mutations in zebrafish. With support from the NIH and guidance from ...have NOT yet been tested for activity as ZFNs in zebrafish. Target Gene Name Zinc Finger Arrays Engineering...and OZ534 OPEN OPEN cCGCTTCAGCggtctGTGGACTCGa zebrafish similar to grp151 (or gpcr-2037) OZ535 and OZ536...PubMed PMID 19798082 . Targeted mutagenesis in zebrafish using customized zinc-finger nucleases. Foley ...

  3. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ... CRISPR Zebrafish CRISPR Plasmids: Zebrafish Browse CRISPR Plasmids ...been designed for use in zebrafish. CRISPR...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps...CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes will ...

  4. EXPRESs Plasmids for Zebrafish Receptors

    Type
    Collection
    ...AVEXIS for screening in zebrafish.... Plasmid Collections EXPRESs Plasmids Zebrafish Receptors EXPRESs Plasmids...Plasmids for Zebrafish Receptors You may also like... Gavin Wright Lab plasmids This is a collection of ...function in early vertebrate development using the zebrafish as a model organism. Please see the following ...

  6. CRISPR References and Information

    Type
    Collection
    ... Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human, rat, cow, dog, pig,...cerevisiae ), worm ( C. elegans ), fruit fly, zebrafish, mouse, rat, and human. CCTop - CRISPR/Cas9 target...tomato, frog ( X. laevis and X. tropicalis ), and zebrafish. Off-Spotter: tool for CRISPR/Cas design Program...Organisms include human, mouse, rat, chicken, frog, zebrafish, fly, worm, Japanese rice fish, maize, Arabidopsis...Arabidopsis, and rice. Proof-of-concept performed in zebrafish. ZiFiT Targeter Originally developed to identify... chicken, frog (X. tropicalis and X. laevis), zebrafish, sea squirt, Drosophila, C. elegans, Arabidopsis... Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human, rat, rabbit, pig, possum...

  7. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TALEN plasmids targeting zebrafish genes.... TALengineering Reagents TALEN Zebrafish Reagents TALENs for Endogenous...Endogenous Zebrafish Gene Targets Recent reports have shown that engineered TALENs can be used to induce...induce targeted mutations with high efficiency in zebrafish ( Sander et al., Nat Biotechnol. 2011 ; Huang ...MGH to target sites within various endogenous zebrafish genes; many of these were generated as part of...effort to provide gene-editing nucleases to the zebrafish community. Target Gene Name TALENs Full Target...

  8. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...for efficiently modifying endogenous genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The...CRISPR-Cas/RGN expression plasmids for use in zebrafish Guide RNA expression plasmids for the EGFP Reporter... Guide RNA expression plasmids for endogenous zebrafish genes...

  9. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps...bacteria, Drosophila, plants, C. elegans, yeast, zebrafish, and Xenopus. Mammalian Plasmid Gene/Insert Promoter...Insert Promoter Selectable Marker PI Publication Zebrafish Plasmid Gene/Insert Promoter Selectable Marker...

  10. Cre-lox system

    Type
    Collection
    ...61391 pCS2-Cre.zf1 Cre codon modified for zebrafish CMV Zebrafish Burgess 61570 Pvalb-2A-Cre targeting vector...Venus Elastase Zebrafish Stainier 84033 pHD157 mCherry-Cre fusion and Venus fabp10 Zebrafish Stainier 85040...for lineage tracing and mosaic analysis in zebrafish Zebrafish Lewis Additional Resources Cre-dependent ... ins-cre_cryaa-Venus Cre-Venus fusion insulin Zebrafish Stainier 24593 AAV-pgk-Cre codon optimized Cre...Lentiviral Jacks 60877 pMAZe Cre and dtomato hsp70 Zebrafish Lewis 60930 pPL5071_TEF1*-Cre_URA3 Cre expressed...pME-ERT2-Cre-ERT2 Tamoxifen-inducible Cre None Zebrafish Stankunas 82696 pCRE-iRFP670 Cre and iRFP670 PGK...fli1aCreERT2 Cre-ERT2; endothelial reporter fli1a Zebrafish Mercader Huber 108995 pDM1488 Cre; NeoR act15 ...

  11. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps...mammalian systems, bacteria, plants, yeast, and zebrafish. Mammalian Plasmid Gene/Insert Promoter Selectable...Insert Promoter Selectable Marker PI Publication Zebrafish Plasmid Gene/Insert Promoter Selectable Marker...

  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pCS2+8CCerulean - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NCerulean - Zebrafish/Xenopus/C.elegans...Expression pCS2+8CeGFP - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NeGFP - Zebrafish/Xenopus/C.elegans/Sea...pCS2+8CmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus/C.elegans... pCS2+8CmOrange - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmOrange - Zebrafish/Xenopus/C.elegans/... pCS2+8CmCherry - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCherry - Zebrafish/Xenopus/C.elegans/...of GFP and/or mCherry, with neomycin cassette Zebrafish, Sea urchin, Xenopus, and C. elegans Hamdoun Lab...

  13. TALEN Engineering

    Type
    Collection
    ...efficiently in: C. elegans (Wood et al., Science 2011) Zebrafish (Sander et al., Nat Biotechnol. 2011) Rats (Tesson...REAL, REAL-Fast and FLASH TALENs for Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs...

  14. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...has been used to engineer ZFNs that function in zebrafish, plants, and human cells...has been used to engineer ZFNs that function in zebrafish, plants, and human cells ( Maeder et al., Mol ...

  15. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... pT7-gRNA 46759 Zebrafish BsmBI none S. pyogenes Chen and Wente DR274 42250 Zebrafish BsaI none S. pyogenes...U6:gRNA 63156 Zebrafish BseRI none S. pyogenes Zon pDestTol2pA2-U6:gRNA 63157 Zebrafish BseRI none S. ...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps... Lu pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 74009 Zebrafish U6 yes, cut S. pyogenes EGFP Del Bene pUAS:Cas9T2ACre...Cas9T2ACre;U6:sgRNA1;U6:sgRNA2 74010 Zebrafish U6 yes, cut S. pyogenes Cre Del Bene pTE4396 74041 Mammalian...Using Transgenic Expression of Cas9 and sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using ...based pGGDestTol2LC vector. Uses five distinct zebrafish U6 promoters for sgRNA expression. Chen pCFD4-...

  17. Huang Lab CRISPR Plasmids

    Type
    Collection
    ...expression has been shown to be functional in zebrafish embryos and mouse embryos. These plasmids are ...

  18. Zinc Finger Consortium Reagents

    Type
    Collection
    ...Genome Engineering Reagents Zinc Finger Arrays for Zebrafish Genes - Browse pre-constructed zinc fingers targeting...

  19. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps...

  20. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps...
Showing: 41 - 60 of 89 results