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Showing: 201 - 220 of 521 results

  1. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... a destination vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick...sequencing and found 98% of the plasmids were perfectly correct. Because they created the assemblies in...

  2. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...non-target proteins), variability (separate batches can perform differently – happens when the antibody is produced...binding to off-target molecules), reliable (wouldn’t perform differently over time), compatible with the desired...

  3. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...their genes of interest. While recombinases are powerful gene expression tools in and of themselves, their...reduced scale in screens without any reduction in performance compared to larger genome-wide libraries. Find...

  4. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...target sites that flox your genes of interest, a powerful FLEx switch can be created for your specifc experimental...more information. References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck...

  6. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...them. For example, gene knockdown with CRISPR interference (CRISPRi) has been limited to lab-adapted bacteria... Resources on Addgene.org Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read...

  7. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...every work setting and no manager will ever be perfect, but the chances of getting a good manager do tend...at once. Many companies will hire a postdoc to perform a specific project, while others will hire a group...

  8. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...student at the Max Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will...lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology...

  9. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ... NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide...an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating transcription. Sites...

  10. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...protein. The Bender’s popularity was short-lived as powerful molecular graphics packages were soon developed...channel.   He then said “I hadn’t realized how perfectly flat the complex is on this surface”….where the...

  11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...have its limitations, especially when trying to perform high-throughput experiments. If a user wants to... modified to tag hundreds of genes, generating powerful genetic screening libraries. Additional protein...

  12. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...through Cas3. After these initial steps, Cas3 can perform its helicase and nuclease functions simultaneously...likes to bluff, making you into think it’s more powerful than it is. The nuclease domain of Cas3 is considered...

  13. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...allows for a wide variety of screens, including interference, activation, and knockout. Most CRISPR screens...CRISPR Delivery Genome-wide Screening Using CRISPR Performing In Vivo CRISPR Screens Using the FITS Approach...

  14. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...just be an artifact and eliminate sources of interference, but the result persisted. (Even better, multiple...Although a ~1% change in signal might not make a very powerful tool, it was promising enough to keep exploring...

  15. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...antibodies (absolutely perfect in every way)? Unfortunately, no antibody is that perfect. One of the known ...

  16. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...program you didn’t know much about could be your perfect fit! Even if you had planned a campus visit and...like sickness or scheduling issues can and do interfere with those in-person plans. Virtual options are...

  17. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions of all distributed...plasmids as part of quality control, but does not perform functional tests. The newest genome engineer sharing...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...from organism to organism. MMEJ doesn’t yield a “perfect repair” like HDR, but it’s much more predictable...ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter, and the...

  19. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...discuss how CRISPR libraries are being used to perform genome-wide screens and highlight some of the reagents...What are the steps involved in a CRISPR screen? Performing a forward genetic screen using CRISPR libraries...

  20. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ... when they begin flowering. It is important to perform the transformation not long after flowering begins...2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in ...
Showing: 201 - 220 of 521 results