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  3. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...they exist but are unreliable for a variety of reasons (Baker, Nature). Tagging your protein allows you...

  4. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...     Morelock, M. M., Rothlein, R., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R.,...

  5. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ... will not stably integrate (unless you used a transposon system, CRISPR targeted strategy, etc.) and episomal...

  7. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...to add overhangs to your insert of interest for Gibson assembly. The steps following primer design and...

  9. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors...

  18. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...  Rachel Leeson...

  19. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies may just not perform as...

  21. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...properties of or draw an amino acid or peptide for some reason. It contains all twenty amino acids plus selenocysteine...

  27. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... is based on the principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla...

  28. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...thanks to our panel of experts, Tim Miles, David Goertson, Xinhong Chen, and Miggy Chuapoco, and to all ...

  30. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...monitor with biosensors? You can keep tabs on my personal favorite (the caffeine of the cell!)—ATP. There...

  39. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it can also detect...
Showing: 481 - 520 of 626 results