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Showing: 651 - 698 of 698 results

  1. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification...

  2. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

  3. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...gene! STEP 2: Generate an Expression Clone When making the expression clone, it is important to choose...

  6. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...

  10. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...t simply rely on chance (or random diffusion) to make this happen.  There are several different versions...

  11. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...bbdone with our bbduk process.    At first, to make sure we understood the concept of channels and implemented...

  12. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...work stalls. We become so fixated with the idea of making something the best it can be that it seems impossible...

  13. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ... and high specificity of anti-epitope antibodies make this approach appealing, but the disadvantages must...

  14. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...retrograde direction from the synapse to the cell body, making it a great tool to study neuronal connectivity....

  15. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...//doi.org/10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved...

  16. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...often looks like the graph on the left, it might make more sense once you realize events are plotted on...

  17. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... than engineering an entirely new protein. This makes it much cheaper to use Cas9 activators. This larger...

  18. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...gRNA targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and cons of...

  19. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... generally ensure cell line identity and purity making it easier for comparable studies to be performed...

  21. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...reproducibility controls". Biological replicates are what makes up your sample size (aka your n value)" and should...

  23. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...exposed side chain that can be easily manipulated to make reactive species without affecting the avidin binding...

  26. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...gating uses the same color for different markers, making it easy to gate on the negative cell population...

  29. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    .... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...

  30. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...iGECI and NIR-GECO2G, and its small size (~1500 bp) makes iGECInano suitable for AAV packaging.    Find iGECInano...

  31. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic mouse lines...

  33. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ... high degree of variability in the process could make it difficult to obtain meaningful results.  Biological...

  34. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...adds a repressible element to this binary system (making this a repressible binary system). The GAL80 repressor...

  36. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...overhangs that allow them to bind. Ligase is used to make bonds between the insert and backbone covalent.  ...

  37. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...Addgene’s Website and travel to meet scientists, making my job different every day. I love the freedom ...

  38. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... and do not require high sequencing read counts making them fast and viable options for multiplex sequencing...

  44. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...cytometry can help you overcome these challenges and make these types of experiments easier and faster to ...

  48. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...) and minimizing the set of plasmids required to make singly or multiply phosphorylated proteins (B40 ...
Showing: 651 - 698 of 698 results