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  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...kDa). This system also requires less genetic engineering than some of the other systems reviewed here....

  2. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...type-specificin vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831...

  3. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...if the plasmid will be within my lab's budget? Ordering: Check the Price of course! The price of some ...

  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...Quick Guide to All Things Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...the expression level in a specific system (by measuring the fluorescence intensity); and 2) to visualize...

  9. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...requires animal sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For...

  13. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...FLAG) are cleaved by specific proteases without requiring the addition of an independent cleavage recognition...

  14. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...pseudotyping and is not HIV-derived. Get Tips on Titering Your Lentivirus Concerning safety of lentiviral...

  16. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ..., and it is exchanged with the gene of interest during the BP or LR reactions. Since the CcdB protein ...

  22. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... reviewing and testing several platforms for engineering pipelines, we selected Nextflow, a workflow management...

  25. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies are also...

  28. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...and plasmid backbone. Because you lose some DNA during the gel purification step, it is important to digest...

  29. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...validating edits.  Mismatch cleavage assays rely on pairing between the edited strand and wild-type strand ...
Showing: 801 - 832 of 832 results