Addgene

Bringing Sustainable Practices to the Lab: Recycling

Type

Blog Post

Published

Feb. 14, 2019, 2:35 p.m.

... Leila Haery...

Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

Type

Blog Post

Published

June 19, 2018, 1:38 p.m.

          ... by water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy.
The Shapiro lab recently...
        

Addgene at the ASM Microbe 2016 Conference

Type

Blog Post

Published

July 7, 2016, 2:30 p.m.

          ... with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being the Director...
        

History of CRISPR Cas - A tale of survival and evolution

Type

Blog Post

Published

Nov. 25, 2013, 10:12 p.m.

          ...families have been characterized. To date, 83% of archaeal and 45% of bacterial genomes utilize this system...
        

Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

Type

Blog Post

Published

April 25, 2019, 3:01 p.m.

          ...blog

Resources on Addgene.org

Learn about the Michael Davidson fluorescent protein collection
Check out...

New and Upcoming Viral Vectors - Spring 2019

Type

Blog Post

Published

March 26, 2019, 1:40 p.m.

... Leila Haery...

Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

Type

Blog Post

Published

Dec. 13, 2018, 1:14 p.m.

          ...PubMed PMID: 22154564.
6. Stoneham, Charlotte A., Michael Hollinshead, and Amin Hajitou. "Clathrin-mediated...
        

Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Type

Blog Post

Published

June 9, 2016, 2:30 p.m.

          ...

3. Swarts, Daan C., et al. “Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that...

New and Upcoming Viral Vectors - September 2019

Type

Blog Post

Published

Sept. 12, 2019, 1 p.m.

... Leila Haery...

New and Upcoming Viral Vectors - June 2019

Type

Blog Post

Published

June 18, 2019, 1:15 p.m.

... Leila Haery...

Improving Transduction Efficiency with Exosome AAVs

Type

Blog Post

Published

March 14, 2019, 1:54 p.m.

          ...coagulation factor IX in liver in a mouse model of Haemophilia B (Meliani et al., 2017). Exo-AAV9 was successfully...
        

FPbase: A new community-editable fluorescent protein database

Type

Blog Post

Published

May 16, 2018, 1 p.m.

          ... plasmids
Find plasmids for FRET
Check out the Michael Davidson Fluorescent Protein Collection

...

Cultivating Community Science at BosLab

Type

Blog Post

Published

Nov. 2, 2017, 1:35 p.m.

          ... first yeast transformations!
Dennis Oleksyuk, Raphael Laurenceau, and Tami Pudina have initiated a soil...
        

How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

Type

Blog Post

Published

June 20, 2017, 2:30 p.m.

... Leila Haery...

Viral Vectors 101: The AAV Retrograde Serotype

Type

Blog Post

Published

April 27, 2023, 1:15 p.m.

          ...neurons! 
This post was originally written by Leila Haery in 2017 and significantly updated in 2023 by Jason...
        

Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Type

Blog Post

Published

June 6, 2019, 12:41 p.m.

          ...miRNA-mediated gene regulation
Article contributed by Michael Lemieux
 Listen to the miRNA reporter podcast segment...
        

Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Type

Blog Post

Published

Dec. 6, 2018, 1:34 p.m.

          ...novel peptide ligand motifs
Article contributed by Michael Lemieux
 Listen to the SH3 domain podcast segment...
        

Top Requested Lentivirus and AAV of 2016

Type

Blog Post

Published

Jan. 6, 2017, 3:56 p.m.

... Leila Haery...

Visualizing Protein Turnover In Situ

Type

Blog Post

Published

Jan. 16, 2018, 3:20 p.m.

          ...PubMed Central PMCID: PMC4414919.
5. Yarmolinsky, Michael B., and L. Gabriel. "Inhibition by puromycin of...
        

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Type

Blog Post

Published

Dec. 8, 2016, 3:30 p.m.

... Michael G. Lemieux...

Plasmids 101: The Wide World of Natural Plasmids

Type

Blog Post

Published

Oct. 8, 2024, 1:15 p.m.

          ...think of bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA...
        

Viral Vectors 101: Chemogenetics

Type

Blog Post

Published

July 11, 2023, 1:15 p.m.

          ... Gomez J, Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent chemogenetics...
        

Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Type

Blog Post

Published

April 6, 2021, 12:30 p.m.

          ...References
Guerin K, Rego M, Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman...
        

A Guide to Designing a Scientific Poster: Content, Layout, and More

Type

Blog Post

Published

Oct. 22, 2019, 12:20 p.m.

... Michael G. Lemieux...

Rabies and Neuronal Tracing

Type

Blog Post

Published

May 29, 2018, 1:51 p.m.

... Leila Haery...

Adapting Toehold Switches to Detect Zika Virus

Type

Blog Post

Published

Aug. 30, 2016, 2:30 p.m.

          ...Previous Collin's Lab Member and Sample 6 Cofounder Michael Koeris
Learn How Minigenomes Are Being Used to ...
        

Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Type

Blog Post

Published

Dec. 1, 2015, 3:30 p.m.

          ...No conference would be complete without CRISPR
Michael Terns from of the University of Georgia studies...
        

Hot Plasmids: May 2026

Type

Blog Post

Published

May 5, 2026, 1:15 p.m.

          ...create unique vast numbers of potential TCRs. The Michael Birnbaum Lab aimed to address the challenge of ...
        

Degrading DNA with Cascade-Cas3

Type

Blog Post

Published

Feb. 11, 2025, 2:15 p.m.

          ...of CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different ...
        

Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

Published

Aug. 21, 2018, 12:31 p.m.

... Michael G. Lemieux...

AAV Vector Quality Control: Going the Extra Mile with NGS

Type

Blog Post

Published

Sept. 12, 2017, 1:44 p.m.

          ....2005.06.003
Guerin K, Rego M, Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman...
        

Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Type

Blog Post

Published

June 22, 2017, 2:30 p.m.

          ...Endogenous Gene Tagging in Human Cells
Check Out the Michael Davidson Fluorescent Protein Collection
Get Tips...
        

Fluorescent Proteins 101: Fluorescent Protein Timers

Type

Blog Post

Published

May 4, 2017, 2:30 p.m.

          ...19136976.
5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation...
        

MXS Chaining

Type

Blog Post

Published

Feb. 7, 2017, 3:30 p.m.

... Leila Haery...

Better Dyeing Through Chemistry & Small Molecule Fluorophores

Type

Blog Post

Published

Sept. 8, 2016, 2:30 p.m.

          ...Fluorescent Protein Fusions
Request Plasmids from the Michael Davidson Collection
Find Fluorescent Protein Kits...
        

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

Published

March 17, 2016, 2:30 p.m.

          ...24093048. PubMed Central PMCID: PMC3787895.
6. Weik, Michael, et al. "The Ebola virus genomic replication promoter...
        

Finding nucleic acids with SHERLOCK and DETECTR

Type

Blog Post

Published

April 16, 2020, 1 p.m.

          ...Barnes KG, Chak B, Mondini A, Nogueira ML, Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL, Bosch...
        

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

Published

Feb. 18, 2020, 2:15 p.m.

          ...Dill-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael D. L. Johnson, Tiffany Lowe-Power, Eric Griffis...
        

AAV Titers: Where Do They Come From And What Do They Mean?

Type

Blog Post

Published

Nov. 15, 2017, 3 p.m.

... Leila Haery...

Transferable Skills Guide: Career Planning Resources

Type

Blog Post

Published

Nov. 14, 2017, 2 p.m.

... Michael G. Lemieux...

Healthcare Consulting: A Door to the Business of Life Sciences

Type

Blog Post

Published

Oct. 13, 2016, 2:30 p.m.

          ...with New Careers while in Grad School
Listen to Michael Koeris' Journey from Grad Student to Entrepeneur...
        

The Challenges of Cell Culture

Type

Blog Post

Published

May 5, 2016, 2:30 p.m.

          ...Lacks. New York: Crown Publishers, 2010.
2. Gold, Michael. "A conspiracy of cells." State University of New...
        

CRISPR 101: Validating Your Genome Edit

Type

Blog Post

Published

Nov. 3, 2022, 12:15 p.m.

          ...https://doi.org/10.1093/bioinformatics/btz867
Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J...
        

Harnessing Bacterial Toxins for Allelic Exchange

Type

Blog Post

Published

Aug. 15, 2019, 12:30 p.m.

          ... (2004): 246-255. PubMed PMID: 15109831.
Pyne, Michael E., et al. "Coupling the CRISPR/Cas9 system with...
        

Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

Published

March 27, 2018, 1:32 p.m.

          ...postnatal administration of AAV vector cures haemophilia B mice." Scientific Reports 7.1 (2017): 4159....
        

Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

Published

June 23, 2016, 2:30 p.m.

          ...et al. "RNA-dependent cysteine biosynthesis in archaea." Science 307.5717 (2005): 1969-1972. PubMed PMID...
        

Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

Published

Jan. 16, 2025, 2:15 p.m.

          ...Gaj, T., Lavzin, M., Ritola, K. D., Lindo, S., Michael, S., Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen...
        

Viral Vectors 101: Optogenetic Tools

Type

Blog Post

Published

June 27, 2023, 1:15 p.m.

          ...blue light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to ...
        

Viral Vectors 101: AAV Variables That Matter

Type

Blog Post

Published

June 1, 2023, 1:15 p.m.

          ...doi.org/10.3389/fnmol.2014.00076
Nieuwenhuis, B., Haenzi, B., Hilton, S., Carnicer-Lombarte, A., Hobo, B...
        

Recombinase-based State Machines Enable Order-dependent Logic in vivo

Type

Blog Post

Published

July 28, 2016, 2:30 p.m.

          ...Vectors Can Be Used for Genome Editing
Learn about Michael Koeris' Journey from Grad Student to SynBio Entrepeneur...
        

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