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We narrowed to 102 results for: Gene Tools Blue

Showing: 1 - 20 of 102 results

  1. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ... have one or more blue flame plasmids, with more added to the list weekly. The Blue Flame Award, first...Michigan says, “Addgene has transformed the way in which we share plasmids and other molecular tools with the...Occasionally, a plasmid tool is so influential that it is requested from Addgene many times as the technology...more blue flame plasmids and have not yet received your award, please contact awards@addgene.org to claim...photo, tag @Addgene and #happydepositor on Twitter. All Blue Flames started with a deposit. Begin yours...particular our Blue Flame Award winners- we’d like to thank you for sharing your plasmids at Addgene. You are...Resources on Addgene.org Start a Deposit Find Additional Hot Plasmids Find the Full List of Blue Flame Depositors...

  2. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...of Freiburg. This paper is about a new tool called BLADE (blue light-inducible AraC dimers in E. coli)...domain that dimerizes upon exposure to blue light (Fig. 1). Another blue light-inducible dimerization domain...this plasmid with your gene of interest, you would replace mCherry with your gene of interest. pBLADEONLY...permission.   BLADE is just one of the several optogenetic tools that Di Ventura’s lab and Khammash's lab have...coli) that turns expression on and off with blue light.   Inspired by @UriAlonWeizmann, here my song about...needed for most applications.   Figure 1: With blue light, the VVD domains of the BLADE dimerize and...already have a pBAD33-based plasmid expressing your gene of interest, all you have to do is co-transform ...

  3. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...refresher.  The tools below use the following optogenetic modules: VVDfast homodimerizes under blue light iLID-SspB...under blue light BcLOV4 undergoes a conformational change and binds the plasma membrane under blue light...The Chow Lab has also generated a variety of membrane-recruitment optogenetic tools for manipulating Rho... CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection? Check...wiggling back and forth under blue light — but there’s a lot more to optogenetics than neuronal activity. Researchers... we’ll highlight a few recently-deposited optogenetic tools to control the cytoskeleton and cell membrane...heterodimerizes or forms clusters under blue light Check out our Optogenetics Guide to learn more about how these...

  4. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...of the optogenetic tools that are still missing. Optogenetic tools for neuroengineering Addgene: For those...want them to use these tools, so we can focus on the next round. Addgene: What tools are still missing from...sensitive to blue light. Many have tried to make green molecules, but then alongside blue they actually...make the blue light molecule much, much, much more light-sensitive. Then you can use dim blue light and... Getting these tools out into the world is a way for us to focus on building new tools, not solving thousands...through optogenetics research Addgene: What are some of the big problems that optogenetic tools can help...necessary for. We published our first paper on optogenetic tools for neuroscience back in 2005, both activators...

  5. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... insert the FP gene into the target gene’s native locus, but introducing large genetic changes in C. elegans...protein. The team validated this tool by targeting six different genes whose protein products have distinct...examining the ratio of blue to red cells. Cells undergoing rapid cell cycles have a high blue to red ratio, whereas...glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library at Addgene CasRx ...cassette exons and disrupts genes with alternative exons. Get the library from Addgene New from the viral...library inhibits over 18,000 human genes. Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed...up here.  Here's what you'll find in this post: Tools for labeling excitatory inhibitory synaptic proteins...

  6. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ... five blue flame plasmids covering three different epigenetic effectors. The diversity of tools is especially... already earned a blue flame for its adenine base editing capabilities. Epigenetics: CRISPR has made it...Truncated gRNAs for Regulating Gene Expression and CRISPR 101: Epigenetics and Editing the Epigenome (plus...plasmid pages that cover some of the hottest CRISPR tools. Base Edit: Base editing plasmids allow you to ...possible to design programmable epigenome editing tools, and the field has certainly taken notice! Our newest...expression backbone with U6 promoter has already earned a blue flame. RNA Editing: Did anyone predict that we’d...problem - see our recap below! Day 1: gRNA design tools: How to Design your gRNA for CRISPR Genome Editing...

  7. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ... activity as a biological tool, Niopek and colleagues turned to optogenetics. Through the use of light-sensitive...switching via a novel optogenetic variant of AcrIIA4.” CASANOVA responds to blue light quickly. Within...exist in nature, but developing them into optogenetic tools can be difficult because this requires detailed...in vivo. They deposited the resulting optogenetic plasmid tools which includes plasmids encoding a fusion...developed a system to control CRISPR-Cas9 activity using blue light (Bubeck et al., 2018). They accomplished this...inhibits CRISPR activity.   With the addition of blue light, the terminal helices of the LOV2 domain unfold...compatible approach to simultaneously clone two genes-of-interest (GOI) into two independent expression...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...CRISPR and optogenetic techniques to develop a variety of photoactivatable CRISPR tools. These tools allow ...during blue light exposure (470nm), the researchers showed that expression of the human ASCL1 gene could...particular interest since blue light has limited applications in vivo. UV and blue light is readily absorbed...sphaeroides Cas9, dCas9 Gene editing Zhou et al., 2018   pdDronpa Cas9, dCas9 Gene editing, transcription...looking to activate, repress, or modify a gene, you now have the tools at your disposal to control your genome... genetically encoded tools, such as microbial opsins, to control cellular activities using light. In 2015...light-controlled CRISPR tools available to researchers - some readily found at Addgene. Shining light on...

  9. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...oligomerization of target proteins in response to blue light. This tool, CRY2clust, was created by adding a 9-residue...Science (IBS) has developed another useful optogenetic tool that enables robust and efficient oligomerization...to the soCoChR podcast segment Optogenetic stimulation is a powerful tool used by neuroscientists to interrogate...CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes a conformational change that...researcher can cause them to interact by stimulation with blue light.  Similarly, light can also be used to control...oligomerization happens within seconds after a pulse of blue light, and is reversible upon termination of light...the extra peptide. CRY2clust should prove a useful tool to further probe cellular function. The CRY2clust...

  10. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...affinity in the dark but dissociate under blue light. This tool has now been modified by Torsten Wittmann...a set of tools that can be used to visualize RNA localization in mammalian cells. These tools consist ...cells, microtubule growth can be switched off with blue light which causes LOV2 and Zdk1 to dissociate due...photodissociable EB1 could be stopped in areas with blue light - a finding which opens novel ways in manipulating...Biology. 2018 PubMed PMID: 30061719. New tools for developing new FPs - the mCarmine story Article contributed...help accelerate the expansion of genome editing tools, the Reuben Harris lab has deposited a reporter ...exhibiting its versatile utility in quantifying multiple gene editing activities. Click here to find the ACE ...

  11. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...light-based genetic tools for studying bioelectricity in vivo. Here are some optogenetic and GEVI tools (as discussed... However, several genetic tools have been developed to address these hurdles. Tools for studying bioelectricity...ion-pump encoding genes HCN1 KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction... Aurora and Phobos optogenetic tools - A collection of bi-stable anion optogenetic channels which can ...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric...Arch (green-light activated proton pump) and ChR2 (blue-light activated cation channel) light-activated ...(590nm). BLINK2 potassium channel - An optimized blue-light induced synthetic potassium channel. Upon ...

  12. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...expression of their genes of interest. While recombinases are powerful gene expression tools in and of themselves...viral service A fast blue-light inducible Cre for precise control of bacterial gene expression By Meghan...precisely controlled through the use of optogenetics. Optogenetic tools use light to induce conformational ...CRISPR knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the ...up here.  Here's what you'll find in this post: Blue-light inducible Cre for use in bacteria CRISPR-Casɸ...plasmid for recombinase activity were exposed to blue light. Cre-Vvd showed substantially improved activity...Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal and...

  13. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...CTD, yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue.   The REC lobe binds tightly...CTD, yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue.   DNA sequence recognition...CTD, yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue; target DNA strand, light gray...Figure 6. NUC lobe, green; REC lobe, light blue; guide RNA, dark blue; DNA, gray; PAM site, yellow. Created... (PAM)? Cas9 is becoming an ever more ubiquitous tool for genome engineering, and studying its structure... red; RuvC, orange; CTD, yellow; REC lobe, light blue; bridge helix, purple.   Apo Cas9 changes conformation...

  14. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors To focus on a particular gene or protein, check out our gene-specific biosensors. These constructs...Examples include blue flame mitochondria-targeted calcium sensor CMV-mito-GEM-GECO1  and blue flame extracellular...Biosensors (‘biological sensors’) are biological tools that monitor a process or detect a given molecule...organization and activating the fluorescent signal. Blue flame GECO biosensors from Robert Campbell’s lab...biosensors are another common class of biosensors. Blue flame “cameleon” calcium sensors created by Roger...Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical...

  15. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...Luminescent molecules are very useful tools because we can easily detect and measure the light they emit...fluorescent proteins like GFP came to be used as research tools is particularly interesting. Although humans have...first, which he called aequorin, gives off a faint blue light when it binds calcium ions, and the second...second, which we now call GFP, absorbs that blue light and glows green. In the late 1980s, another researcher...new green glowing protein could be used to measure gene transcription, as well as to track protein localization... he was right! He began to study the A. victoria gene responsible for encoding GFP, and, in 1992, he reported...and began to realize its potential as a research tool. Soon, the number of people studying it grew. In...

  16. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...Download the blue plasmid background.     You can find all of the plasmids that Addgene offers by visiting...❤️ DNA at Addgene, we're presenting you with another DNA background option, Download the blue DNA background...coronavirus collection page, where you can find plasmid tools to accelerate your COVID-19 research.  Download ...any scientist, whether you use molecular biology tools in your research or not! And learn more about the... chances are you are a fan of Addgene or our very cute mascots!  Blugene has been helping us make Plasmids...default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing 11 bold ... purple bacteria video background with mascots Blugene and Aavery? Download the 720p version or download...

  17. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...readership of the Addgene blog in 2023. Views from a country are represented in blue; darker blue indicates more.... Views from a country are represented in blue; darker blue indicates more views from that country. Stats...help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols ...barriers for scientists wanting to share their tools with Addgene.  In our next twenty years, we can’t be certain...them. As early as 2007, we began grouping similar tools together in what would eventually become our collection... to easily access and compare their options for tools such as empty backbones, lentiviral packaging systems...learn what Addgene can do to help. More resources on the Addgene blog Twenty Years of Addgene Sharing: ...

  18. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...fluorescence when excited with 488nm blue light. The T203H mutation in PA-GFP generates a chromophore population ...fold increase in fluorescence when illuminated with blue light (1). Similar to PA-GFP, scientists later developed...photoconverts from green to red fluorescence when exposed to blue light, which is less phototoxic to live tissue than...560 High 580/ 591 Dendra2 Monomer UV-Violet or blue Green/ Red 4,500 High 553/ 573 PA-mCherries Monomer...photoactivation) (6). Dronpa is photoactivated by blue wavelengths of light, causing emission of green ...green fluorescence, but then further exposure to blue light also inactivates the chromophore, leading to a...reactivated by exposure to UV light. The fact that blue light both activates and inactivates Dronpa is not...

  19. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...of these visualization tools is CRISPR-Sirius, the brightest CRISPR-based tool to date for visualizing..., or boxB sequences inserted into the sgRNA generates blue, green, or red colors for labeling. A combination...resources on the Addgene blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all ...years, dCas9 has also become a robust visualization tool to study the spatial and temporal arrangement of...Previous iterations of genomic loci visualization tools using CRISPR In 2015, Thoru Pederson’s lab at the...identical hairpins within one gRNA will result in a blue, green, or red readout. But gRNAs containing two...improved on previous CRISPR-based loci visualization tools by increasing gRNA stability. First, they began ...

  20. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...detect gene copy number based on the number of fluorescent puncta observed. By labeling two genes of a ... composite of fluorescent measurements from red, blue, and green channels overlaid on a bright-field image...vivo. Catalytically dead Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed targeting...Chen et al. successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the...also monitor the spatial relationship of the two genes over time, at a range of distances from 2-75 Mbp...Scaling up to 6 colors + white Color Hairpin Blue MS2-MS2 Green PP7-PP7 Red boxB-boxB Cyan MS2...identical hairpin sequences specify the primary colors blue, green, and red. In contrast, a gRNA with two different...
Showing: 1 - 20 of 102 results