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Showing: 61 - 80 of 193 results
  1. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... particles to your solution while still using a volume that’s compatible with your experimental setup.... the filter - When using a concentration filter column, make sure to rinse off the surface of the filter...
  2. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...new NEBNext Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching...prior NEBNext Ultra II DNA Library Prep Kit for Illumina, one was only able to extract libraries from previously...
  3. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...assemble hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences...prepared as plexWell™ libraries, sequenced on an Illumina® sequencer, and then analyzed with our plasmid...
  4. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...calcium dynamics in the cytosol and sarcoplasmic reticulum are of interest to muscle biologists, while neuroscientists... Overview of AAV Encoded Calcium Biosensors Illuminating Epigenetics with a FRET Based Biosensor Fluorescent...
  5. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...stored either in a dark, opaque vial or wrapped in aluminum foil to prevent ambient light from degrading them...
  6. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...either through a chromogenic or chemiluminescent reaction. Chemiluminescence, which is light produced by ...
  7. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...direct the fluorescent protein to the Endoplasmic Reticulum (ER). If homodimerization occurs (most likely ...structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are visible. These whorls can be...
  8. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...management software aren’t just limited to large volume facilities; it can be useful in academic labs where...collision. It may even be worth it to have extra columns in your database to the effect of `dev_name` and...
  9. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... expected range. For example, calcium in the ER lumen reaches the sub-millimolar range, much higher than...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...
  10. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016...
  11. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...metal-affinity chromatography, usually using a Ni2+ column. Like other affinity tags, a His tag can be fused...with the cleavage reaction taking place between column runs. The TEV protease itself, with various mutations...
  12. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical Center. DNA transposons are...currently a PhD candidate in the Sternberg Lab at Columbia University Medical Center. He currently studies...
  13. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...= {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies/uL V = Volume of virus in reaction mix D = Dilution...
  14. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...oviducts. After solution injection into the oviductal lumen, tweezer-type electrodes are placed to mediate electroporation...i-GONAD is that it requires a high-concentration, column-purified DNA template.   Many thanks to Channabasavaiah...
  15. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...dark areas (protected) as opposed to brightly illuminated ones (risky). They also prefer to explore novel...References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability...
  16. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...Master. Note: BRET= Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer...
  17. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...primer to each template. The PCR product is then column purified. Degrade wild-type template strand: Next...23766324. PubMed Central PMCID: PMC4409680. 2. Mccullum, E. O., Williams, B. A., Zhang, J., & Chaput, ...
Showing: 61 - 80 of 193 results