...primer to each template. The PCR product is then column purified. Degrade wild-type template strand: Next...23766324. PubMed Central PMCID: PMC4409680. 2. Mccullum, E. O., Williams, B. A., Zhang, J., & Chaput, ...

...trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. https://doi.org/10.1093...CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https://doi.org/10.1093...

... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...

...qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini et al., 2012) measures...homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls. This assay...

... sequencing, (which differ from the short-read Illumina MiSeq sequencing that Addgene uses.) However, ...article.   Long-read sequencing Addgene uses Illumina MiSeq NGS to perform whole plasmid sequencing ...

...sensitivity, and photostability under two-photon illumination than previous GEVIs. Some constructs are also...00916-3. doi: 10.1016/j.cell.2022.07.013 pYtags illuminate RTK signaling Receptor tyrosine kinases (RTKs...

...recently developed a new method called DULIP (dual luminescence-based co-immunoprecipitation) to overcome the... this kit are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being used for... pooled library Parasites from the apicomplexan phylum cause severe human and livestock diseases such ...

...so don’t be shy and reach out! Someone from your alumni community is a great place to start. What is science.... Many fellowship programs can connect you with alumni before and during your application process so you...

...PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations... probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical Sciences 29.7 (2013...

...(Erdmann et al., 2019). Check out our recent Illuminating Choices blog post for more discussion about ...from Addgene Better Dyeing Through Chemistry Illuminating Choices: A Guide to Selecting Fluorescent Dyes...

... humidity, sunlight exposure time, and watering volume on total crop production (though you would not ...Carlo Simulation Study. Front. Appl. Math. Stat. Volume 2 (2016). doi.org/10.3389/fams.2016.00002  ...

...visualize the protein via fluorescence or chemiluminescence, precipitate the protein out of solution, ...antibody allows the protein to be detected by chemiluminescence or fluorescence. Western blots are frequently...

...great for teachers looking to supplement their curriculum. From the details about personal protective equipment...

...Light Interval System, or FLIS, incorporates a bioluminescence protein into the chitin that can be activated...

...from lab gloves. The tree was adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape...

... Press and is designing a genetic engineering curriculum at MIT. He also writes Codon, a blog about biotechnology...

...Challenge, Westenberg’s course includes Winogradsky columns and characterizing the microbes from the termite...

...London with me as his first graduate student.  As alumni (he as a postdoc, I as a technician) of Bob Weinberg...

...the "Resource Information" heading in the right column of the plasmid page. Check out the purple arrow...

...for interesting connections is your university’s alumni database. Sharing a school is a little connection...