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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 29 results for: CIL

Showing: 1 - 29 of 29 results

  1. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient genome editing tool that allows ...lysogeny broth (LB) agar plate with 100 μg/ml ampicillin and incubate O/N at 37 °C. Pick 2 - 3 colonies...all steps consists of DMEM supplemented with 2% penicillin/ streptomycin and 1% L-glutamine, which is used... per well for a final volume of 150 μl. This facilitates screening by allowing a multichannel pipette ...

  2. Viral Production

    Type
    Collection
    ...performed by the facility that produced the viral vector lot. Contact us to determine which facility produced ...collaboration with viral vector manufacturing facilities, such as the University of Pennsylvania Vector...

  3. Deisseroth INTRSECT Collection

    Type
    Collection
    ...cholecystokinin interneurons in the basal amygdala facilitates fear extinction. eNeuro. 6(6). PubMed (Link opens...Expressing Neurons in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing...Expressing Neurons in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...harnessed the type II CRISPR nuclease system to facilitate genome editing in mammalian cells (Cong et al...AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top...hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination and fluorescence assisted...

  5. Bacterial Expression Systems

    Type
    Collection
    ... purified through affinity chromatography. To facilitate the purification process, proteins are often ... reduces bacterial protein contamination and facilitates extraction. Browse our most popular plasmids ...and periplasmic protein expression in E. coli , Bacillus subtilis , and Pichia pastoris . The EcoFlex MoClo...

  6. Synthetic Biology - Overview

    Type
    Collection
    ...leading synthetic biologists around the world to facilitate the sharing of parts and information. Addgene...Multiplexed/Programmable Gene Networks Mascher Lab Bacillus BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt...

  7. Microbiology Resources

    Type
    Collection
    ...islandicus Plasmids for Bacteria Species Anabaena sp. Bacillus sp. Burkholderia sp. Bordetella pertussis Campylobacter...sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. Haemophilus influenzae Helicobacter pylori...

  8. AAV Packaged on Request

    Type
    Collection
    ...pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-quality quality...all-inclusive of several important steps: MTA facilitation for the transfer plasmid, including legal approval...

  9. Open Enzyme Collection

    Type
    Collection
    ...marker for all plasmids in this collection is ampicillin. Find more information on the enzymes and protocols...BGT beta-glucosyltransferase 165531 pOpen-UDG UDG Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine...

  10. COVID-19 Resources

    Type
    Collection
    ...cell protein 4) is involved in beating of airway cilia.( 7 ) Cytokines - SARS-CoV-2 infection induces a... - Kogiso H, et al, 2020, Int J Mol Sci Airway Ciliary Beating Affected by the Pcp4 Dose-Dependent [Ca...

  11. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Voeltz 89472 GFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 GFP Ken-Ichi Takemaru 89770 pLenti-EGFP-hChibby1...pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 EGFP Ken-Ichi Takemaru 118084 pLenti-EB1-EGFP...

  12. Brain Initiative Collection

    Type
    Collection
    ...Recombinant mAb Plasmid Collection . The NeuroMab Facility and James Trimmer have produced monoclonal antibodies...Service . Addgene provides a viral service to help facilitate scientific research. Addgene's AAV are made from...

  13. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...The NeuroMab Facility and James Trimmer have produced monoclonal antibodies validated for neuroscience...Protocols References The UC Davis/NIH NeuroMab Facility (Link opens in a new window) together with the...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary...collection . Protocols The UC Davis/NIH NeuroMab Facility has several protocols available that may be helpful...

  14. Cre-lox system

    Type
    Collection
    ...higher levels in mice than P1 bacteriophage Cre, facilitating high rates of Cre-driven recombination. CREM... knocked in to a mouse at the Rosa26 locus to facilitate Cas9 mediated genome editing in multiple tissues...

  16. Immunology Research Plasmids and Resources

    Type
    Collection
    ...8 CKLFSF8, CKLFSF8-V2 CNTF ciliary neurotrophic factor HCNTF CNTFR ciliary neurotrophic factor receptor... IL1RRP2 IL1RN interleukin 1 receptor antagonist ICIL-1RA, IL-1ra3, IL1F3, IL1RA, IRAP, MGC10430 IL2 interleukin...IL1RRP2, IL36R IL1RN interleukin 1 receptor antagonist ICIL-1RA, IL-1ra3, IL1F3, IL1RA, IRAP, MGC10430 IL2 interleukin...

  17. Antibodies

    Type
    Collection
    ...distributes ready-to-use recombinant antibodies to help facilitate scientific research. We are building a collection...

  19. Mammalian RNAi Tools

    Type
    Collection
    ...RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows...

  20. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...BSL-3) agent, SARS-CoV-2 requires specialized facilities for the study of the live virus. Alternatively...

  22. Antibody Plasmid Collection

    Type
    Collection
    ...amber codons in scFvs A new expression vector facilitating production and functional analysis of scFv antibody...

  23. Worm Expression Resources

    Type
    Collection
    ...Seydoux lab website . Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed...

  24. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...44251 pgRNA-bacteria BBa_J23119 SpeI + HindIII Ampicillin none, need Cas9 plasmid Qi Do you have suggestions...

  25. Arf GTPase Family

    Type
    Collection
    ...membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid droplets...

  26. Viral Vectors

    Type
    Collection
    ...delivery has many advantages for research. Viruses facilitate the delivery of genetic information to hard-to-transfect...

  28. Plasmids for Stem Cell Research

    Type
    Collection
    ...BCL2L1, and EBNA1 from three separate plasmids A Facile Method to Establish Human Induced Pluripotent Stem...

  29. Plan Your Experiment

    Type
    Collection
    ...efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Scientific ...
Showing: 1 - 29 of 29 results