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Showing: 1 - 16 of 16 results
  1. Synthetic Biology - Yeast

    Type
    Collection
    ...PP295Expresses GFP. Figure 3....PP296Expresses GFP. Figure 3....PP297Expresses GFP. Figure 3....PP298Expresses GFP. Figure 3....PP299Expresses GFP. Figure 3....PP300Expresses GFP. Figure 3....PP318Expresses GFP. Figure 3....pMV-TRP1standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which...pAV10.K4pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs...could be released and ligated to series of TUsTRP1 (Saccharomyces cerevisiae)Synthetic Biology...Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less...
  2. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...pAWp7-14pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128]UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128...pAWp7-2pFUGW-UBCp-RFP-CMVp-GFP-[miR-128]UBCp-RFP-CMVp-GFP-[miR-128]Lentiviral Lu...Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less...pSS9Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coligfpuv (...pAV10.K4pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs...(unpublished) p404ADH1Bacterial Expression, Yeast ExpressionTRP1...(unpublished) p404CYC1Bacterial Expression, Yeast ExpressionTRP1...(unpublished) p404TEF1Bacterial Expression, Yeast ExpressionTRP1...(unpublished) p404TDH3Bacterial Expression, Yeast ExpressionTRP1...
  3. Antibodies

    Type
    Collection
    ...mAID fused to lysine-less vhhGFP4 (mAID-nanobody)mAID-nanobody (Synthetic) Mansfeld...117718pCS2+_HA-mAID-nanobody (Kless)mRNA synthesis of lysine-less HA-mAID-nanobdy for...117713pCDNA5FRT/TO_HA-mAID-nanobodyExpression of tetracycline-inducible HA-tagged lysine-less...(MoonTag-Nb-GFP)Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells....CRY2 PHR E490G fused to anti-GFP nanobody for optogeneticsCRY2 PHRolig-VHH(GFP) (Arabidopsis thaliana...)express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogeneticsCRY2 PHR-VHH(GFP) (Arabidopsis...nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins....ferritinExpresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin...117718pCS2+_HA-mAID-nanobody (Kless)mRNA synthesis of lysine-less HA-mAID-nanobdy for...fused to TRPV1 and GFP-ferritin chimera fusion proteintransient receptor potential vanillin 1 (Rattus...mAID fused to lysine-less vhhGFP4 (mAID-nanobody)mAID-nanobody (Synthetic) Mansfeld.../Mucolipin-3 (Human) recombinant mouse monoclonal antibodyanti-TRPML3/Mucolipin-3 (Homo sapiens) recombinant...antibody (Mus musculus) Trimmer 114477N268/19RMammalian Expression of anti-TRPML3...
  4. Cre-lox system

    Type
    Collection
    ...3XUAS-IVS-Cre::PEST Cre expressed at low levels to reduce toxicity; See PI page for other recombinases that are less...Cre and GFP coexpression CAG Retroviral Gage 49054 CAG-GFP/cre Cre-GFP fusion CAG Retroviral Gage...GFP and Cre CAG Mammalian Lu 117148 Hiv7CMV-Cremyc-2A-GFP GFP and Cre CMV Lentiviral Lu 118029...Bacterial Liu 63704 pRetroX GFP T2A Cre GFP and Cre tight TRE Retroviral Foijer 64769 pTW040 Cre...49056 AAV-GFP/Cre Cre-GFP fusion CMV AAV Gage 49111 pEMS1980 iCre with MCS for inserting promoter...on GFP (CRE-DOG) CAG Mammalian Cepko 69573 pCAG-C-CreintG Cre recombinase dependent on GFP (CRE-DOG...iRFP670, Cre, and GFP TRE Mammalian Jacks 68544 AAV-Cre-GFP Cre CMV AAV Nestler 68552 HRE:GFP-pGK...pBS594 promoterless EGFPcre Cre-EGFP fusion in a promoterless vector with several available restriction...Sauer 11958 pBS532 promoterless GFPcre promoterless CRE-GFP fusion none Mammalian Sauer 11960 pBS537...60930 pPL5071_TEF1*-Cre_URA3 Cre expressed at low levels mutant TEF2 Yeast Piper 60931 pPL5608_TEF1*-Cre_TRP1...Cre-GFP fusion EF-1 alpha Mammalian Sauer 11923 pBS598 EF1alpha-EGFPcre Cre-EGFP fusion EF-1 alpha...tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre...
  5. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less...pMV-TRP1standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which...pFAB801BIOFAB reporter plasmid for measuring trp_att_L126 termination efficiencytrp_att_L126 terminator...pSS9Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coligfpuv (...pDL13alcA promoter, lacZ, trpCSynthetic Biology ; A. nidulans Keasling Marker...pDL14gpdA promoter, lacZ, trpCSynthetic Biology ; A. nidulans Keasling Marker...pAV10.K4pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs...could be released and ligated to series of TUsTRP1 (Saccharomyces cerevisiae)Synthetic Biology...
  6. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...gRNA-trp-HYBgBlock product of trp1 deletion gRNA cassette (Synthetic)Yeast Expression...CRISPR ; gRNA Borodina EasyClone-MarkerFree: A vector toolkit for marker-less...gRNA-trp-HYBgBlock product of trp1 deletion gRNA cassette (Synthetic)Yeast Expression...Px461-Cas9n-Trp53-sgRNA-betaTrp53 (Mus musculus)Mammalian Expression, CRISPR Massague...TRPM7 gRNA (BRDN0001148025)TRPM7 (Homo sapiens)Mammalian Expression, Lentiviral, CRISPR...TRPM7 gRNA (BRDN0001146152)TRPM7 (Homo sapiens)Mammalian Expression, Lentiviral, CRISPR...TRPM7 gRNA (BRDN0001147821)TRPM7 (Homo sapiens)Mammalian Expression, Lentiviral, CRISPR...TRPM7 gRNA (BRDN0001145424)TRPM7 (Homo sapiens)Mammalian Expression, Lentiviral, CRISPR...Px458-Cas9n-Trp63-Exon4-1sgRNATrp63 (Mus musculus)Mammalian Expression, CRISPR Massague...
  7. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less...Bacterial Expression Borodina EasyClone-MarkerFree: A vector toolkit for marker-less...SS13 lac-inducible GFP reporter circuitGFPBacterial Expression, Synthetic Biology Sauro...pSB4A5 JQ929581mKate2 (Synthetic), Superfolder GFP (Synthetic)Synthetic Biology ; BioBrick cloning vector...pSB4A5 JQ929582mKate2 (Synthetic), Superfolder GFP (Synthetic)Synthetic Biology ; BioBrick cloning vector...Bxbi GFP BAC Reportersynthetic circuit (Other) Lu Synthetic mixed-signal...TP901 GFP BAC Reportersynthetic circuit (Other) Lu Synthetic mixed-signal...pDL13alcA promoter, lacZ, trpCSynthetic Biology ; A. nidulans Keasling Marker...pDL14gpdA promoter, lacZ, trpCSynthetic Biology ; A. nidulans Keasling Marker...pFAB801trp_att_L126 terminator (Synthetic)Bacterial Expression, Synthetic BiologypLTetO1...pPro24(s)-gfpGFPuv (Other)Bacterial Expression, Synthetic Biology Keasling...SS13 lac-inducible GFP reporter circuitGFPBacterial Expression, Synthetic Biology Sauro...
  8. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Other)Gentamicin Borodina EasyClone-MarkerFree: A vector toolkit for marker-less...L202 gRNAeGFP L202 gRNA (Other)U6 Harris A panel of eGFP reporters for single...L138 gRNAeGFP L138 gRNA (Other)U6 Harris A panel of eGFP reporters for single...gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP....gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP....pCRISPRyl_AXPCas9UAS1B8-TEF(136)LEU2 Wheeldon Standardized markerless...pCRISPRyl_A08Cas9UAS1B8-TEF(136)LEU2 Wheeldon Standardized markerless...
  9. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Other)Gentamicin Borodina EasyClone-MarkerFree: A vector toolkit for marker-less...expression of FnCas9-GFP in bacterial cells pET-His6-GFP-TEV-LIC...Cas9 GFP plasmid constitutively expressed from CAG promoter....col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression....Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone....Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker....Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif....FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid....-TtrpC-CYC1thumanized Cas9 (Homo sapiens)LEU2 Hong Efficient gene editing..., ble (bleomycin resistance marker) (Other)Aspergillus nidulans tef1 promoter, Aspergillus nidulans trpC...hph (hygromycin resistance marker) (Other)Aspergillus nidulans tef1 promoter, Aspergillus nidulans trpC...Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants...with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020...
  10. Worm Expression Resources

    Type
    Collection
    ...-mex3Pmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjectionPmex-5:GFP::H2B::PEST...+ SEC)SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology armsPmyo-2::GFP + SEC (Caenorhabditis...34877pCFJ420 - Peft-3::GFP::H2B (ubiquitous)Peft-3 GFP H2B tbb-2 UTR (Caenorhabditis elegans)...A set of vectors for C. elegans research, including lacZ and GFP fusion vectors....(dpiRNA)::CDK-1cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode....elegans) Kuwabara 38147pPK720dpy-7p::ptc-3::gfp Kuwabara...Backbone pPD95.79 (Firelab).firefly luciferase luc+ fused to GFP (S65C) (Other) Glover...(Homo sapiens) Wandless 82546pCFJ150-set2Pmex-5:GFP::H2B::PEST::set-2...::unc-54_3'UTRPmyo-2::GFP::unc-54_3'UTR (Caenorhabditis elegans) Lehner...::unc-54utrPunc-122::GFP (Caenorhabditis elegans) Jorgensen 19326pCFJ70...- Pmyo-3::twk-18(cn110)::unc-54utrPmyo-3::twk-18(cn110)cDNA (Caenorhabditis elegans)...Jorgensen 19327pCFJ90 - Pmyo-2::mCherry::unc-54utrPmyo-2::mCherry (Caenorhabditis elegans...Caenorhabditis elegans) Jorgensen 19332pJL43.1 - Pglh-2::MosTase::glh-2utrPglh...(Caenorhabditis elegans) Jorgensen 19359pGH8 - pRAB-3::mCherry::unc-54utrPrab...
  11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...GFP for yeast expression pET GFP - C-terminal GFP for bacterial expression...Varies pBABE GFP - Retroviral gene expression pMKO.1 GFP - Retroviral...Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression pcDNA3 GFP LIC cloning...Another common scenario is fusing your protein to another protein, such as GFP, which allows you...vector - C-terminal GFP for mammalian expression pPS808 - N-terminal...Contains a loxP-flanked cassette pBTG (pBigT-IRES-GFP) - pBigT modified...for transcription and injection pCS2P+ - Transcription from the SP6 promoter pCS2 mt-GFP...including amino acid sequences, see our list of common tags.Back to Top Selectable Markers Regardless...YFP tags for purification or localization experiments Yeast GAL4, PGK, ADH1, ADE2, TRP1...pAG306GAL-ccdB - Yeast Gateway expression vector (see more Gateway vectors) TRP1...Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression pcDNA3 GFP LIC cloning...
  12. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...pCXLE-dCas9VPH-T2A-GFP-shP53dCas9VPH-T2A-GFP-shP53...pCXLE-dCas9VPPH-T2A-GFP-shP53dCas9VPPH-T2A-GFP-shP53...Cas9 GFP plasmid constitutively expressed from CAG promoter....col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression....EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP....Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif....P2A-EGFP (Synthetic)CAG Kleinstiver Unconstrained genome targeting with near-PAMless...3xFLAG-dCas9/pMXs-puro3xFLAG-dCas9 (Synthetic)LTRPuromycin...MSCV_Cas9_puroCas9 (Synthetic)MSSV_LTRPuromycin...FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid....targeting with near-PAMless engineered CRISPR-Cas9 variants Science...targeting with near-PAMless engineered CRISPR-Cas9 variants Science ,...
  13. Synthetic Biology - Bacterial

    Type
    Collection
    ...pHL1853PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA GFP (Other), tetR...pHL1855PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrA GFP (Other), tetR...pHL1529PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA GFP (Other), tetR, csrA...pHL1757PconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetR GFP (Other), tetR (Other)Bacterial Expression...pHL 246AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1 GFP (Other)Bacterial Expression...pHL1914PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR GFP (Other), tetR (Other)Bacterial Expression...pHL 1073pCon:ompC::mCherry, pLlacO-1:sodB::gfp mCherry (Other), GFP (Other)Bacterial Expression, Synthetic...pFAB801BIOFAB reporter plasmid for measuring trp_att_L126 termination efficiencytrp_att_L126 terminator...pSS9Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coligfpuv (...biosensorpstS (A197C) (Other)Bacterial Expression Webb Development of a Reagentless...(Other)Bacterial Expression Webb Development of a range of fluorescent reagentless...C106A R286C Q457C K488A) (Other)Bacterial Expression Webb A Fluorescent, Reagentless...
  14. Plasmids for Optogenetics Research

    Type
    Collection
    ...pAAV-Syn-FLEX-Mac-GFP Inhibitory Mac AAV GFP Boyden 58853 pAAV-Syn-Mac-GFP Inhibitory Mac AAV GFP...Inhibitory Mac AAV GFP Boyden 58841 pAAV-Syn-CsChR-GFP Excitatory CsChR AAV GFP Boyden 58849 pAAV-CaMKII-Mac-GFP...Arch AAV GFP Boyden 26929 AAV-CAG-ChR2-GFP Excitatory ChR2 AAV GFP Boyden 26966 pAAV-Ef1a-DIO...Inhibitory ArchT AAV GFP Boyden 29777 pAAV-CAG-ArchT-GFP  Inhibitory ArchT AAV GFP Boyden 29778...Excitatory Chronos AAV GFP Boyden 58806 pAAV-Syn-ChrimsonR-GFP Excitatory ChrimsonR AAV GFP Boyden...59056 pCAG-FLEX-rc[Chronos-GFP] Excitatory Chronos Mammalian GFP Boyden 59070 pAAV-Syn-CoChR-GFP...Inhibitory Jaws AAV GFP Boyden 65016 pAAV-hsyn-Jaws-GFP-ER2 Inhibitory Jaws AAV GFP Boyden 65454..._2A_GFP_WPRE_bGHpA LITE AAV GFP Zhang 49570 pBMN HAYFP-LOV24 LOV Retroviral YFP Wandless 49800...Mammalian mCherry Tucker 75372 CRY2(535, L348F)-mCherry Cry2-CIB1 Mammalian mCherry Tucker 78210 pDBTrp-LexABD-CRY2FL...-GFP Excitatory ChR2 AAV GFP Boyden 59325 pRVdG-4ArchT-EGFP Inhibitory ArchT Rabies Virus GFP Wickersham...GFP Boyden 58841 pAAV-Syn-CsChR-GFP Excitatory CsChR AAV GFP Boyden 58849 pAAV-CaMKII-Mac-GFP Inhibitory...CIBN-GFP-TMD Cry2-CIB1 Mammalian EGFP De_Camilli 79572 Lyn11-CIBN-GFP Cry2-CIB1 Mammalian EGFP De_Camilli...Mac AAV GFP Boyden 58851 pAAV-EF1a-FLEX-ArchT-GFP Inhibitory ArchT AAV GFP Boyden 58852 pAAV-Syn-FLEX-Mac-GFP...
  15. Luciferase Plasmids

    Type
    Collection
    ...Firefly and Renilla luciferase are less-than-ideal protein tags due to their large size....Wilson 22522 phGluc Gaussia EF1α Expression of Gaussia luciferase; GFP is expressed if cells are...Gambhir 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP...11323pRL-TK 4x mut GFP2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP...Bruchez 105538 pENN.AAV.TBG.PI.ffLuciferase.RBG Firefly TGB AAV expression of firefly luciferase and GFP...Merlino 71394pFUGW-Pol2-ffLuc2-eGFPLentiviral vector of luciferase-eGFP fusion gene driven...vector of luciferase-eGFP fusion gene driven by FerH promoterFerH-ffLuc2-eGFP (Mus musculus)...Unless otherwise noted, these plasmids are meant for expression in mammalian systems.   ...Costello 85126pAM4663NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless...Enhancer Element (DE) (Homo sapiens) Jaenisch 26530pmiReport PU.1 3' UTRPU...3'UTR mutNETO2 3'UTR mutant (Homo sapiens) Bartel 14497pIS55 POGK 3'UTRPOGK...
  16. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...expression of FnCas9-GFP in bacterial cells pET-His6-GFP-TEV-LIC...Contains a GFP-dropout cassette and a direct repeat....(unpublished) Constitutive dCas9 and GFP...target RNA sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless..., ble (bleomycin resistance marker) (Other)Aspergillus nidulans tef1 promoter, Aspergillus nidulans trpC...hph (hygromycin resistance marker) (Other)Aspergillus nidulans tef1 promoter, Aspergillus nidulans trpC...pCas9-CR4Cas9 nuclease Prather The no-SCAR (Scarless...pUC-fFuCas9-HTBNLS-hphCas9 and hygromycin resist gene (Other)gpdA;trpC...
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