AAV Production in HEK293 Cells
Type
Protocol
...7.0. *Pro-Tip* The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only...complete media and mix. Seed all cells in 1 CF2. Return to incubator for 48 h. Trypsinize and resuspend...Seed 250 millions cells from the CF2 into 1 CF5. Return to incubator for 24 h - 36 h. Proceed with transfection...new one before putting the CF5 in the incubator. Return the CF5 to the incubator for 96 h. This incubation...minutes on ice between each pulse, 50% amplitude. Return to ice between each sonication to avoid overheating...