LOCUS       pcDNA3.1-MS2-NLS        8104 bp    DNA     circular SYN 17-DEC-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|717623449|
COMMENT     VNTDBDATE|717623449|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA3.1-MS2-NLS-Tet1 CD-Flag|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pcDNA3.1-MS2-NLS-Tet1 CD-Flag" 0 8104 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "BamHI" "KpnI" "NotI" "XhoI" "EcoRI" "NheI" "HindIII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 8104 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1425 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 922 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3454 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 3361 0 3489 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1217 0 4866 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 896 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 912 0 1668 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "Amp(R)" 4 0 1 7108 7968 0 (CStringList) 
COMMENT            (CStringList "/invitrogen=\"1020000\"") 1 1 1 1 
COMMENT            "complement(5173..6033)") 
COMMENT        #8=(CFSignal (CObList) "BGH pA" 26 0 0 3531 3755 0 (CStringList) 
COMMENT            (CStringList "/invitrogen=\"1870000\"") 1 1 1 1 "1596..1820") 
COMMENT        #9=(CFSignal (CObList) "BGH reverse primer" 27 0 1 3525 3542 0 
COMMENT            (CStringList) (CStringList "/invitrogen=\"1910000\"") 1 1 1 1 
COMMENT            "complement(1590..1607)") 
COMMENT        #10=(CFSignal (CObList) "bla promoter" 30 0 1 7969 8067 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2330000\"") 1 1 1 1 
COMMENT             "complement(6034..6132)") 
COMMENT        #11=(CFSignal (CObList) "CMV forward primer" 27 0 0 769 789 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1930000\"") 1 1 1 1 
COMMENT             "769..789") 
COMMENT        #12=(CFSignal (CObList) "CMV promoter" 29 0 0 232 819 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2180000\"") 1 1 1 1 "232..819") 
COMMENT        #13=(CFSignal (CObList) "f1 origin" 33 0 0 3801 4229 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2500000\"") 1 1 1 1 "1866..2294") 
COMMENT        #14=(CFSignal (CObList) "Hygro(R)" 4 0 0 4622 5647 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1180000\"") 1 1 1 1 "2687..3712") 
COMMENT        #15=(CFSignal (CObList) "pUC origin" 33 0 1 6290 6963 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2540000\"") 1 1 1 1 
COMMENT             "complement(4355..5028)") 
COMMENT        #16=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4234 4604 0 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2290000\"") 1 1 1 1 
COMMENT             "2299..2669") 
COMMENT        #17=(CFSignal (CObList) "SV40 pA" 25 0 0 5777 5907 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1840000\"") 1 1 1 1 "3842..3972") 
COMMENT        #18=(CFSignal (CObList) "T7 primer" 27 0 0 863 882 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2100000\"") 1 1 1 1 "863..882") 
COMMENT        #19=(CFSignal (CObList) "T7 promoter" 30 0 0 863 879 0 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2370000\"") 1 1 1 1 "863..879") 
COMMENT        #20=(CFSignal (CObList) "Flag" 21 0 0 3461 3484 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "MS2 coat protein (N55K)" 21 0 0 923 1312 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #22=(CFSignal (CObList) "NLS" 21 0 0 1361 1393 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #23=(CFSignal (CObList) "Tet1 CD" 21 0 0 1433 3451 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #24=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #25=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                    "pcDNA3.1-MS2-NLS-Tet1 CD-Flag" 2) 5 "" 0 4) 
COMMENT              #26=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pcDNA3.1-MS2-NLS-Tet1 CD-Flag'" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 12/17/14 07:50\317\302\316\347" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 12/17/14 07:50\317\302\316\347" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8104 bp" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Division: SYN" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 20-MAR-2011" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #39=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #40=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 145457000 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #41=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1425 " 1))))) "BamHI: 1 site") 1 5 
COMMENT                          "GGATCC" "CCTAGG") 
COMMENT                     #43=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 145456872 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #44=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1217 " 1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 4866 " 1))))) "EcoRI: 2 sites") 1 
COMMENT                          5 "GAATTC" "CTTAAG") 
COMMENT                     #47=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 145456808 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #48=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 912 " 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 1668 " 1))))) "HindIII: 2 sites") 
COMMENT                          1 5 "AAGCTT" "TTCGAA") 
COMMENT                     #51=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 145456968 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #52=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 922 " 1))))) "KpnI: 1 site") 5 1 
COMMENT                          "GGTACC" "CCATGG") 
COMMENT                     #54=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 145456840 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #55=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 896 " 1))))) "NheI: 1 site") 1 5 
COMMENT                          "GCTAGC" "CGATCG") 
COMMENT                     #57=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 145456936 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #58=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 3454 " 1))))) "NotI: 1 site") 2 6 
COMMENT                          "GCGGCCGC" "CGCCGGCG") 
COMMENT                     #60=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 145456904 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #61=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 3361 " 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 3489 " 1))))) "XhoI: 2 sites") 1 5 
COMMENT                          "CTCGAG" "GAGCTC"))) "Restriction/Methylation Map") 
COMMENT              #64=(CIvgnResourcePar 
COMMENT                   (CLinePar (CParagraph 1021 (0 0) 0 2 0 0 180) 
COMMENT                    "<a href=\"ivgnlink:/iq\">Invitrogen products related to pcDNA3.1-MS2-NLS-Tet1 CD-Flag</a>" 
COMMENT                    1) 2 "" 0 0) 
COMMENT              #65=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #66=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #67=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4622 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4622  End: 5647" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2687..3712" 1))) "Hygro(R)") 
COMMENT                            #72=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7108 (3 #7# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7108  End: 7968 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5173..6033)" 1))) 
COMMENT                                 "Amp(R)"))) "CDS (2 total)") 
COMMENT                     #76=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #77=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 923 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 923   End: 1312" 1))) 
COMMENT                                 "MS2 coat protein (N55K)") 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1361 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1361  End: 1393" 1))) "NLS") 
COMMENT                            #81=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1433 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1433  End: 3451" 1))) "Tet1 CD") 
COMMENT                            #83=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3461 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3461  End: 3484" 1))) "Flag"))) 
COMMENT                          "Misc. Feature (4 total)") 
COMMENT                     #85=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 25 (7 25 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #86=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5777 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5777  End: 5907" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3842..3972" 1))) "SV40 pA"))) 
COMMENT                          "PolyA Signal (1 total)") 
COMMENT                     #90=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 26 (7 26 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3531 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3531  End: 3755" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1596..1820" 1))) "BGH pA"))) 
COMMENT                          "PolyA Site (1 total)") 
COMMENT                     #95=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #96=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 769 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 769   End: 789 " 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   769..789" 1))) "CMV forward primer") 
COMMENT                            #100=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 863 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #101=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 863   End: 882 " 1) 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   863..882" 1))) "T7 primer") 
COMMENT                            #104=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 3525 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 3525  End: 3542 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(1590..1607)" 1))) 
COMMENT                                  "BGH reverse primer"))) "Primer (3 total)") 
COMMENT                     #108=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #109=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 232 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 232   End: 819 " 1) 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   232..819" 1))) "CMV promoter") 
COMMENT                             #113=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4234 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4234  End: 4604" 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2299..2669" 1))) 
COMMENT                                   "SV40 early promoter"))) 
COMMENT                           "Promoter Eukaryotic (2 total)") 
COMMENT                     #117=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #118=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 863 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 863   End: 879 " 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   863..879" 1))) "T7 promoter") 
COMMENT                             #122=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7969 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7969  End: 8067 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(6034..6132)" 1))) 
COMMENT                                   "bla promoter"))) 
COMMENT                           "Promoter Prokaryotic (2 total)") 
COMMENT                     #126=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #127=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3801 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3801  End: 4229" 1) 
COMMENT                                     #129=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1866..2294" 1))) "f1 origin") 
COMMENT                             #131=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6290 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #132=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6290  End: 6963 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #134=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(4355..5028)" 1))) 
COMMENT                                   "pUC origin"))) 
COMMENT                           "Replication Origin (2 total)"))) "Feature Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #135=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #136=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #137=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #138=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #139=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #140=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #141=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #142=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #143=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #144=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #145=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #146=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #147=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #148=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #149=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #150=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #151=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #152=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #153=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #154=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #155=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #156=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #157=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #158=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #159=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #160=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #161=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #162=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #163=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #164=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #165=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #166=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #167=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #168=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #169=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #170=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #171=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #172=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #173=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #174=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 
COMMENT         -6169) 1 1 0 1 1 
COMMENT        (mapper: 18.9475 -17.9685 40.0378 40.0378 0.01 10 -1 8104 8104 1 0 0) 
COMMENT        #175=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 0 100) 
COMMENT              (CObjectList 
COMMENT               #176=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1153 100) 
COMMENT                     (CObjectList 
COMMENT                      #177=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #141# 145324732 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27496 6.27296 1 0.0214037) 0.0774085) 
COMMENT                      #178=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #138# 57730132 100) 
COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "pcDNA3.1-MS2-NLS-Tet1 CD-Flag" 
COMMENT                            "@N" 1 0 0.871165 0 -10 20.0061 1.39868 Nil) 
COMMENT                      #179=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #137# 268889064 100) 
COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "8104 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 2.59755 0.799245 Nil)) (CObjectList)) 
COMMENT               #180=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil 2082537722 100) 
COMMENT                     (CObjectList 
COMMENT                      #181=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -874 100) 
COMMENT                            (CObjectList 
COMMENT                             #182=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #139# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 0.105276 0.771763 1 
COMMENT                                    0.082322) 0.8 1.8 1) 
COMMENT                             #183=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Amp(R)" "@N" 1 
COMMENT                                   -8.03275 12.1963 0 -10 3.172 1.04901 #182#) 
COMMENT                             #184=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #139# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 1.90193 2.69614 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #185=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #136# 50927188 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Hygro(R)" "@N" 
COMMENT                                   1 -12.6282 -8.71165 0 -10 3.72148 1.04901 
COMMENT                                   #184#)) (CObjectList)) 
COMMENT                      #186=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -1019 100) 
COMMENT                            (CObjectList 
COMMENT                             #187=(CScratch 
COMMENT                                   (CWidget 0 (3 #20# 0) 1 2 0 0 #144# 56061872 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 3.59485 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #188=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #136# 2082602632 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Flag" "@N" 1 
COMMENT                                   7.54091 -12.1963 0 -10 1.94816 1.04901 #187#) 
COMMENT                             #189=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #21# 0) 1 2 0 0 #140# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 5.25759 5.55948 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #190=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "MS2 coat protein (N55K)" "@N" 1 18.2598 
COMMENT                                   3.48466 0 -10 10.4901 1.04901 #189#) 
COMMENT                             #191=(CScratch 
COMMENT                                   (CWidget 0 (3 #22# 0) 1 2 0 0 #144# 56063856 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 5.22043 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #192=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 
COMMENT                                   14.3722 0 0 -10 1.87323 1.04901 #191#) 
COMMENT                             #193=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #23# 0) 1 2 0 0 #140# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.60182 
COMMENT                                    5.1647 1 0.082322) 0.8 1.8 0) 
COMMENT                             #194=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #136# 27525437 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Tet1 CD" "@N" 1 
COMMENT                                   14.6194 -5.22699 0 -10 3.37181 1.04901 #193#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #195=(CGroupWidget 
COMMENT                            (CWidget 25 (7 25 0) 1 2 0 0 Nil -112 100) 
COMMENT                            (CObjectList 
COMMENT                             #196=(CLine 
COMMENT                                   (CWidget 0 (3 #17# 0) 1 2 0 0 #155# 801 100) 
COMMENT                                   (LOGPEN 0 6 32768) 8 0.835356 1.70066 1.80207) 
COMMENT                             #197=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 pA" "@N" 1 
COMMENT                                   -15.1675 -1.74233 0 -10 3.79641 1.04901 #196#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #198=(CGroupWidget 
COMMENT                            (CWidget 26 (7 26 0) 1 2 0 0 Nil -251 100) 
COMMENT                            (CObjectList 
COMMENT                             #199=(CScratch 
COMMENT                                   (CWidget 0 (3 #8# 0) 1 2 0 0 #160# 885 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 3.45397 1.9 0.082322 1) 
COMMENT                             #200=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "BGH pA" "@N" 1 
COMMENT                                   6.82746 -17.4233 0 -10 3.44674 1.04901 #199#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #201=(CGroupWidget 
COMMENT                            (CWidget 27 (7 27 0) 1 2 0 0 Nil -1158 100) 
COMMENT                            (CObjectList 
COMMENT                             #202=(CScratch 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #144# 56101056 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 3.54531 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #203=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #136# 25821776 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "BGH reverse primer" "@N" 1 10.4379 -15.681 0 
COMMENT                                   -10 8.81667 1.04901 #202#) 
COMMENT                             #204=(CScratch 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #144# 885 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.67869 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #205=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "CMV forward primer" "@N" 1 12.7591 13.9386 0 
COMMENT                                   -10 8.81667 1.04901 #204#) 
COMMENT                             #206=(CScratch 
COMMENT                                   (CWidget 0 (3 #18# 0) 1 2 0 0 #144# 56100944 
COMMENT                                    100) (LOGPEN 0 8 13395507) 10 1 5.60592 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #207=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 primer" "@N" 
COMMENT                                   1 11.2137 12.1963 0 -10 4.14608 1.04901 #206#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #208=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -321 100) 
COMMENT                            (CObjectList 
COMMENT                             #209=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #140# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.63921 
COMMENT                                    6.09437 1 0.082322) 0.8 1.8 0) 
COMMENT                             #210=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "CMV promoter" 
COMMENT                                   "@N" 1 9.4619 15.681 0 -10 6.26908 1.04901 
COMMENT                                   #209#) 
COMMENT                             #211=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #140# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.7093 2.99648 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #212=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #136# 51164812 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early promoter" "@N" 1 -10.7423 
COMMENT                                   -12.1963 0 -10 8.91657 1.04901 #211#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #213=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -182 100) 
COMMENT                            (CObjectList 
COMMENT                             #214=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #152# 353 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 
COMMENT                                    0.0286411 0.105276 1 0.082322) 0.8 1.8 1) 
COMMENT                             #215=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #136# 153098888 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "bla promoter" 
COMMENT                                   "@N" 1 -4.51253 13.9386 0 -10 5.56974 1.04901 
COMMENT                                   #214#) 
COMMENT                             #216=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #19# 0) 1 2 0 0 #158# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 5.51866 5.60592) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
COMMENT                             #217=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 14.4865 6.96932 0 -10 5.22007 1.04901 
COMMENT                                   #216#)) (CObjectList)) 
COMMENT                      #218=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -595 100) 
COMMENT                            (CObjectList 
COMMENT                             #219=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #139# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 2.99958 3.33166 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #220=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 origin" "@N" 
COMMENT                                   1 3.2036 -19.1656 0 -10 3.62158 1.04901 #219#) 
COMMENT                             #221=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #139# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 0.883231 1.40496 1 
COMMENT                                    0.082322) 0.8 1.8 1) 
COMMENT                             #222=(CLabel (CWidget 0 (0 0) 1 2 0 0 #136# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pUC origin" 
COMMENT                                   "@N" 1 -14.895 5.22699 0 -10 4.59566 1.04901 
COMMENT                                   #221#)) (CObjectList))) (CObjectList)) 
COMMENT               #223=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1088 100) 
COMMENT                     (CObjectList 
COMMENT                      #224=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -600 100) 
COMMENT                            (CObjectList 
COMMENT                             #225=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #143# 56101896 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 5.17089 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #226=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #145# 38836800 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Bam}HI (1425)" "@N (@S)" 5 16.1432 
COMMENT                                   -1.74233 0 -10 4.59566 0.799245 #225#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #227=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil 1 100) 
COMMENT                            (CObjectList 
COMMENT                             #228=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #143# 1 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.56025 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #229=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #145# 2016814457 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Kpn}I (922)" "@N (@S)" 5 14.7295 
COMMENT                                   5.22699 0 -10 3.62158 0.799245 #228#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #230=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -456 100) 
COMMENT                            (CObjectList 
COMMENT                             #231=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #143# 56460080 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.60027 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #232=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #145# 5617 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Not}I (3454)" "@N (@S)" 5 11.3617 
COMMENT                                   -10.454 0 -10 3.79641 0.799245 #231#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #233=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -1223 100) 
COMMENT                            (CObjectList 
COMMENT                             #234=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #143# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.58038 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #235=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #145# 25821736 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Nhe}I (896)" "@N (@S)" 5 11.4026 10.454 
COMMENT                                   0 -10 3.72148 0.799245 #234#)) (CObjectList)) 
COMMENT                      #236=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #3# 0) 1 2 0 0 Nil -1909 100) 
COMMENT                            (CObjectList 
COMMENT                             #237=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #143# 56115656 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.67226 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #238=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 153100888 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xho}I (3361)" "@N (@S)" 5 13.0377 
COMMENT                                   -8.71165 0 -10 3.79641 0.799245 #237#) 
COMMENT                             #239=(CScratch 
COMMENT                                   (CWidget 2 (1 #3# 2) 1 2 0 0 #143# 186039080 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 3.57318 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #240=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 739 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Xho}I (3489)" "@N (@S)" 5 8.82336 
COMMENT                                   -13.9386 0 -10 4.07115 0.799245 #239#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #241=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #4# 0) 1 2 0 0 Nil -391 100) 
COMMENT                            (CObjectList 
COMMENT                             #242=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #143# 56101952 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 5.3319 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #243=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 51165700 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Eco}RI (1217)" "@N (@S)" 5 15.9516 
COMMENT                                   1.74233 0 -10 3.97125 0.799245 #242#) 
COMMENT                             #244=(CScratch 
COMMENT                                   (CWidget 2 (1 #4# 2) 1 2 0 0 #143# 185046112 
COMMENT                                    100) (LOGPEN 0 6 10053171) 8 1 2.50726 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #245=(CLabel (CWidget 0 (0 0) 1 2 0 0 #135# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Eco}RI (4866)" "@N (@S)" 5 -11.6405 
COMMENT                                   -10.454 0 -10 4.52073 0.799245 #244#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #246=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #6# 0) 1 2 0 0 Nil -735 100) 
COMMENT                            (CObjectList 
COMMENT                             #247=(CScratch 
COMMENT                                   (CWidget 1 (1 #6# 1) 1 2 0 0 #143# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.56799 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #248=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 920 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (912)" "@N (@S)" 5 13.3251 
COMMENT                                   8.71165 0 -10 4.32092 0.799245 #247#) 
COMMENT                             #249=(CScratch 
COMMENT                                   (CWidget 2 (1 #6# 2) 1 2 0 0 #143# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.98279 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #250=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 57730356 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (1668)" "@N (@S)" 5 15.9773 
COMMENT                                   -3.48466 0 -10 4.84542 0.799245 #249#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #251=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -525 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #252=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -316 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) -80457350 
COMMENT       (CStringList) 4288855512 4289974765 (CObList)))
FEATURES             Location/Qualifiers
     CDS             complement(7108..7968)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     polyA_site      3531..3755
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     primer          complement(3525..3542)
                     /invitrogen="1910000"
                     /vntifkey="27"
                     /label=BGH\reverse\primer
     promoter        complement(7969..8067)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     primer          769..789
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        232..819
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     rep_origin      3801..4229
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     CDS             4622..5647
                     /invitrogen="1180000"
                     /vntifkey="4"
                     /label=Hygro(R)
     rep_origin      complement(6290..6963)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        4234..4604
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    5777..5907
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     primer          863..882
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        863..879
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    3461..3484
                     /vntifkey="21"
                     /label=Flag
     misc_feature    923..1312
                     /vntifkey="21"
                     /label=MS2\coat\protein\(N55K)
     misc_feature    1361..1393
                     /vntifkey="21"
                     /label=NLS
     misc_feature    1433..3451
                     /vntifkey="21"
                     /label=Tet1\CD
BASE COUNT     1982 a      2074 c      2074 g      1974 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttggta ccatggcttc taactttact cagttcgttc tcgtcgacaa 
      961 tggcggaact ggcgacgtga ctgtcgcccc aagcaacttc gctaacgggg tcgctgaatg 
     1021 gatcagctct aactcgcgtt cacaggctta caaagtaacc tgtagcgttc gtcagagctc 
     1081 tgcgcagaag cgcaaataca ccatcaaagt cgaggtgcct aaagtggcaa cccagactgt 
     1141 tggtggtgta gagcttcctg tagccgcatg gcgttcgtac ttaaatatgg aactaaccat 
     1201 tccaattttc gctacgaatt ccgactgcga gcttattgtt aaggcaatgc aaggtctcct 
     1261 aaaagatgga aacccgattc cctcagcaat cgcagcaaac tccggcatct acagcgctgg 
     1321 aggaggtgga agcggaggag gaggaagcgg aggaggaggt agccccaaga agaagagaaa 
     1381 ggtggaggcc agcggtggag gaagtggagg tggttcaggt ggaggatccg gcgaagctgc 
     1441 accctgtgac tgtgatggag gtacacaaaa agaaaaaggc ccatattata cacaccttgg 
     1501 ggcaggacca agtgtggctg ctgtcaggga gctcatggag actaggtttg gccagaaggg 
     1561 gaaggcaatc cggattgaga agatagtgtt cacggggaag gaagggaaga gctctcaggg 
     1621 ctgcccggtc gccaagtggg tgatcagaag aagtggtcct gaagagaagc ttatttgttt 
     1681 ggttcgtgag cgtgtagacc atcactgttc gacggctgtg atagttgtcc ttatcctgct 
     1741 gtgggaaggt atccctcgcc tgatggctga ccgcctgtac aaagagctca ctgagaactt 
     1801 gaggtcctac agcggacatc ccacagaccg aagatgtacc ctcaacaaaa agcgtacctg 
     1861 cacctgtcaa ggcatcgacc caaaaacctg cggagcgtcc ttctcctttg gctgttcgtg 
     1921 gagcatgtat ttcaacggct gtaagtttgg gaggagtgaa aaccccagaa aattcagact 
     1981 tgctccaaac taccccttac ataactacta taagagaatt actggaatga gttctgaagg 
     2041 aagtgacgtg aaaaccgggt ggatcattcc agaccgcaag accctcataa gcagagagga 
     2101 aaaacagctt gaaaagaatt tacaagaatt ggctacagta ttagctccac tttacaagca 
     2161 gatggctcca gttgcttatc aaaatcaggt ggaatatgaa gaagttgctg gagactgtcg 
     2221 acttggaaat gaagaggggc gtcctttctc tggtgtcacc tgttgcatgg atttttgtgc 
     2281 ccattctcac aaggacattc acaacatgca caacggaagc accgtggtgt gtacgttgat 
     2341 tcgagcagat ggccgtgaca caaattgtcc cgaggatgaa caactccacg tcctgccact 
     2401 ataccggctt gcagacactg atgaatttgg ctccgtggaa gggatgaagg ccaaaatcaa 
     2461 atctggggcc atccaagtca atgggccaac caggaagagg cgactacgtt ttactgagcc 
     2521 tgttcctcga tgtgggaaga gggccaaaat gaagcagaac cacaataaat caggttcaca 
     2581 caacactaag agcttttcat cagcctcatc tacttctcac ctagtgaaag acgaatctac 
     2641 agacttctgt cccctgcagg cttcctccgc agaaacatct acctgtacgt acagtaaaac 
     2701 agcctcaggt gggtttgcag aaacaagtag tattctccac tgcacaatgc cttctggagc 
     2761 acacagtggt gctaatgcag ctgctgggga atgtactgga acggtgcagc ctgccgaggt 
     2821 ggctgctcat cctcaccagt ctcttcccac agccgattct cccgttcatg ctgagcctct 
     2881 cactagtcca tctgagcagc taacttctaa ccagtcaaac cagcagctcc ctctcctcag 
     2941 caattctcag aaactggctt cctgtcaggt ggaagatgag cggcaccctg aagcggatga 
     3001 gcctcagcac cccgaggacg ataacttgcc tcaacttgat gagttctggt cagacagtga 
     3061 ggagatctac gccgatcctt cctttggtgg cgtggcgata gcacccattc acggctcggt 
     3121 gctcattgag tgcgctcgga aggagcttca tgctaccacc tctttgcgct cccccaaacg 
     3181 aggggtccct tttcgtgtgt cccttgtatt ctaccagcac aaaagcctaa acaagcctaa 
     3241 tcatggtttt gatatcaaca aaattaagtg taaatgcaaa aaagtaacga aaaaaaagcc 
     3301 cgcagaccgg gagtgtcctg atgtatcccc cgaagccaat ttatcacacc aaattccttc 
     3361 tcgagttgca tcaaccttaa cccgagacaa tgttgttacc gtgtccccat actctctcac 
     3421 tcatgttgcg ggaccctaca atcgttgggt cgcggccgca gactacaagg acgatgacga 
     3481 taagtaactc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc 
     3541 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 
     3601 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 
     3661 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 
     3721 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 
     3781 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     3841 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     3901 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat 
     3961 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 
     4021 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     4081 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     4141 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga 
     4201 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 
     4261 ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc tcaattagtc 
     4321 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 
     4381 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 
     4441 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 
     4501 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 
     4561 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt 
     4621 gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga 
     4681 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga 
     4741 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga 
     4801 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat 
     4861 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt 
     4921 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga 
     4981 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg 
     5041 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta 
     5101 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga 
     5161 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg 
     5221 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc 
     5281 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc 
     5341 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc 
     5401 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga 
     5461 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg 
     5521 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg 
     5581 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa 
     5641 ggaatagcac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 
     5701 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 
     5761 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     5821 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     5881 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 
     5941 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 
     6001 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 
     6061 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 
     6121 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 
     6181 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     6241 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
     6301 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     6361 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     6421 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     6481 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     6541 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     6601 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     6661 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     6721 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
     6781 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     6841 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     6901 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     6961 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
     7021 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
     7081 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
     7141 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
     7201 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
     7261 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
     7321 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
     7381 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 
     7441 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
     7501 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
     7561 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
     7621 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
     7681 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 
     7741 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
     7801 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
     7861 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
     7921 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
     7981 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
     8041 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 
     8101 cgtc 
//
