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Reference Icon Redbook 多种 TALEN 试剂盒


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转录激活因子样效应蛋白(TALE) 由12或更多个模块重复组成, 每个识别特异的 DNA碱基对。TALE可与FokI核酸酶融合产生TALEN,后者可用于基因打靶。可定制的TALE和TALEN被证明是DNA操纵和遗传工程的有力工具。建立TALE和TALEN所需的质粒已存放于Addgene,可供学术界索取。

TALE和TALEN 的组装两步过程:

  1. 将12-31个不同的“重复可变双残基”(RVD) 组装为阵列
  2. 克隆到pTAL骨架中,与特定催化域融合

TALEN优点

  • 被高特异性并可定制
  • 组装快捷,在约1周内构建定制TAl效应子!
  • 异地针对低量
  • 独特靶点预测约每35个碱基对
TALEN-fig.png

TALEN 试剂盒:

TALEN 试剂盒 说明 存放实验室
Golden Gate TALEN 2.0 Golden Gate克隆方法。在多个生物中验证。 Dan Voytas and Adam Bogdanove
Joung Lab TAL 通过连续连接组装。 在斑马鱼中验证 Keith Joung
Zhang Lab TALE 工具箱 PCR/Golden Gate克隆方法。优化人胞。 Feng Zhang
LIC TAL Effector 组装套件 大会通过结扎独立克隆(LIC)。 验证人胞。 Veit Hornung
Musunuru/Cowan Lab TALEN 试剂盒 套件包括834质粒
预组装的三聚体和四聚体RVD域。
Kiran Musunuru and Chad Cowan

使用与其他质粒Golden Gate TALEN 2.0:

适用于 质粒 存放实验室
Golden Gate TALEN 2.0 RCIscript-GoldyTALEN and pC-GoldyTALEN Daniel Carlson
Golden Gate TALEN 2.0 TAL5-BB and pTAL6-BB Tom Ellis
Golden Gate TALEN 2.0 pCS2TAL3-DD and pCS2TALE3-RR David Grunwald
Golden Gate TALEN 2.0 pCAG-T7-TALEN(Sangamo)-目的地构造 Pawel Pelczar
Golden Gate TALEN 2.0 TALEN 构建与评价配件包 Takashi Yamamoto