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Individual REAL TALE Repeat Plasmids


The Joung Lab recently described a rapid and robust protocol for constructing TALE repeat arrays of any desired sequence, referred to as the REAL (Restriction Enzyme And Ligation) assembly method (Sander et al., Nat Biotechnol. 2011). REAL utilizes a set of 28 plasmids each encoding a single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays.

These 28 plasmids are available individually or as a set as part of the Joung Lab REAL Assembly TALEN Kit, which also includes the four TALEN expression vectors.

More detailed information about REAL, including detailed and up-to-date protocols, can be found on the TALengineering website. Users may also wish to join the TALengineering newsgroup.

ID Plasmid
32250 TAL006
32251 TAL007
32253 TAL009
32254 TAL010
32255 TAL011
32256 TAL012
32257 TAL014
32258 TAL015
32259 TAL016
32260 TAL017
32261 TAL019
32262 TAL020
32263 TAL021
32264 TAL022
32265 TAL024
32266 TAL025
32267 TAL026
32268 TAL027
32269 TAL029
32270 TAL030
32271 TAL031
32272 TAL032
32273 TAL034
32274 TAL035
32275 TAL036
32276 TAL037
32277 TAL039
32278 TAL040