Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

Open Source Wnt Project Wnt Logo

Open Source Wnt Project Plasmids
(Kit # 1000000022 )

Depositing Labs:   Marian Waterman, David Virshup

The Open Source Wnt Kit is a 95-plasmid library of all 19 Wnt clones. The collection contains full-length open reading frames (ORFs) for every human Wnt ligand.

This kit consists of 1 plate, and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80oCelsius immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$375 USD + shipping
Available to Academic and Nonprofits Only

Original Publication

A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML. Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PubMed.

Disulfide Bond Requirements for Active Wnt Ligands. MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. J. Biol. Chem.. 2014 May 19; doi:10.1074/jbc.M114.575027 PubMed.

Description

The Open Source Wnt Project was initiated to create a collection of standardized Wnt plasmids that are easily accessible to the research community. The project and plasmids were created by Dr. Marian Waterman's group at UC Irvine and Dr. David Virshup's group at Duke Singapore as a way to pool community resources and efforts to develop an accessible and affordable set of reagents for the Wnt community. The Open Source Wnt Kit is a 95-plasmid library of all 19 Wnt clones. The collection contains full-length open reading frames (ORFs) for every human Wnt ligand.

In addition to this original set of 76 clones, Dr. Bryan MacDonald and Dr. Xi He have contributed a modified version of each tagged Wnt that enables epitope tagging without loss of Wnt signaling activity.

To read Addgene's Septemeber 2013 interview with Marian Waterman, Crowd-Sourced Science: The Open Source Wnt Project, click here.

Kit Documentation

Icon Information and Plate Map PDF (96.8 KB)

Resources

Icon Open Source Wnt Info Sheet (94.4 KB)

Icon Open source Wnt Nomenclature (42.5 KB)

Icon Wnts in pcDNA3.2 V5-DEST Map (4.5 MB)

Icon Wnts in pENTR D-TOPO Map (1.9 MB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

"The plasmid kit used in this publication was a gift from Marian Waterman, David Virshup and Xi He (Addgene kit # 1000000022)"

For your Reference section:

A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities. Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, Waterman ML. Differentiation. 2012 Sep;84(2):203-13. doi: 10.1016/j.diff.2012.06.004. Epub 2012 Jul 9. PubMed.

Disulfide Bond Requirements for Active Wnt Ligands. MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. J. Biol. Chem.. 2014 May 19; doi:10.1074/jbc.M114.575027 PubMed.

Resistance Color Key:

 
  Kanamycin  
 
  Ampicillin  

Inventory

Well Plasmid Resistance
A / 1 Wnt1 STOP
  Kanamycin
A / 2 Wnt2 STOP
  Kanamycin
A / 3 Wnt2B2 STOP
  Kanamycin
A / 4 Wnt3A STOP
  Kanamycin
A / 5 Wnt3 STOP
  Kanamycin
A / 6 Wnt4 STOP
  Kanamycin
A / 7 Wnt5A STOP
  Kanamycin
A / 8 Wnt5B STOP
  Kanamycin
A / 9 Wnt6 STOP
  Kanamycin
A / 10 Wnt7A STOP
  Kanamycin
A / 11 Wnt7B STOP
  Kanamycin
A / 12 Wnt8A STOP
  Kanamycin
B / 1 Wnt8B STOP
  Kanamycin
B / 2 Wnt9A STOP
  Kanamycin
B / 3 Wnt9B STOP
  Kanamycin
B / 4 Wnt10A STOP
  Kanamycin
B / 5 Wnt10B STOP
  Kanamycin
B / 6 Wnt11 STOP
  Kanamycin
B / 7 Wnt16 STOP
  Kanamycin
B / 8 Wnt1 Non-STOP
  Kanamycin
B / 9 Wnt2 Non-STOP
  Kanamycin
B / 10 Wnt2B2 Non-STOP
  Kanamycin
B / 11 Wnt3A Non-STOP
  Kanamycin
B / 12 Wnt3 Non-STOP
  Kanamycin
C / 1 Wnt4 Non-STOP
  Kanamycin
C / 2 Wnt5A Non-STOP
  Kanamycin
C / 3 Wnt5B Non-STOP
  Kanamycin
C / 4 Wnt6 Non-STOP
  Kanamycin
C / 5 Wnt7A Non-STOP
  Kanamycin
C / 6 Wnt7B Non-STOP
  Kanamycin
C / 7 Wnt8A Non-STOP
  Kanamycin
C / 8 Wnt8B Non-STOP
  Kanamycin
C / 9 Wnt9A Non-STOP
  Kanamycin
C / 10 Wnt9B Non-STOP
  Kanamycin
C / 11 Wnt10A Non-STOP
  Kanamycin
C / 12 Wnt11 Non-STOP
  Kanamycin
D / 1 Wnt16 Non-STOP
  Kanamycin
D / 2 Wnt10B Non-STOP
  Kanamycin
D / 3 pcDNA-Wnt2
  Ampicillin
D / 4 pcDNA-Wnt2B2
  Ampicillin
D / 5 pcDNA-Wnt3A
  Ampicillin
D / 6 pcDNA-Wnt3
  Ampicillin
D / 7 pcDNA-Wnt4
  Ampicillin
D / 8 pcDNA-Wnt5A
  Ampicillin
D / 9 pcDNA-Wnt5B
  Ampicillin
D / 10 pcDNA-Wnt6
  Ampicillin
D / 11 pcDNA-Wnt7A
  Ampicillin
D / 12 pcDNA-Wnt7B
  Ampicillin
E / 1 pcDNA-Wnt8A
  Ampicillin
E / 2 pcDNA-Wnt8B
  Ampicillin
E / 3 pcDNA-Wnt9A
  Ampicillin
E / 4 pcDNA-Wnt9B
  Ampicillin
E / 5 pcDNA-Wnt10A
  Ampicillin
E / 6 pcDNA-Wnt10B
  Ampicillin
E / 7 pcDNA-Wnt11
  Ampicillin
E / 8 pcDNA-Wnt16
  Ampicillin
E / 9 pcDNA-Wnt1-V5
  Ampicillin
E / 10 pcDNA-Wnt2-V5
  Ampicillin
E / 11 pcDNA-Wnt2B2-V5
  Ampicillin
E / 12 pcDNA-Wnt3A-V5
  Ampicillin
F / 1 pcDNA-Wnt3-V5
  Ampicillin
F / 2 pcDNA-Wnt4-V5
  Ampicillin
F / 3 pcDNA-Wnt5A-V5
  Ampicillin
F / 4 pcDNA-Wnt5B-V5
  Ampicillin
F / 5 pcDNA-Wnt6-V5
  Ampicillin
F / 6 pcDNA-Wnt7A-V5
  Ampicillin
F / 7 pcDNA-Wnt7B-V5
  Ampicillin
F / 8 pcDNA-Wnt8A-V5
  Ampicillin
F / 9 pcDNA-Wnt8B-V5
  Ampicillin
F / 10 pcDNA-Wnt9A-V5
  Ampicillin
F / 11 pcDNA-Wnt9B-V5
  Ampicillin
F / 12 pcDNA-Wnt10A-V5
  Ampicillin
G / 1 pcDNA-Wnt10B-V5
  Ampicillin
G / 2 pcDNA-Wnt11-V5
  Ampicillin
G / 3 pcDNA-Wnt16-V5
  Ampicillin
G / 4 pcDNA-Wnt1
  Ampicillin
G / 5 Active Wnt1-V5
  Ampicillin
G / 6 Active Wnt2B-V5
  Ampicillin
G / 7 Active Wnt2-V5
  Ampicillin
G / 8 Active Wnt3A-V5
  Ampicillin
G / 9 Active Wnt3-V5
  Ampicillin
G / 10 Active Wnt4-V5
  Ampicillin
G / 11 Active Wnt5A-V5
  Ampicillin
G / 12 Active Wnt5B-V5
  Ampicillin
H / 1 Active Wnt6-V5
  Ampicillin
H / 2 Active Wnt7A-V5
  Ampicillin
H / 3 Active Wnt7B-V5
  Ampicillin
H / 4 Active Wnt8A-V5
  Ampicillin
H / 5 Active Wnt8B-V5
  Ampicillin
H / 6 Active Wnt9A-V5
  Ampicillin
H / 7 Active Wnt9B-V5
  Ampicillin
H / 8 Active Wnt10A-V5
  Ampicillin
H / 9 Active Wnt10B-V5
  Ampicillin
H / 10 Active Wnt11-V5
  Ampicillin
H / 11 Active Wnt16-V5
  Ampicillin
Data from: CALCULATED @ 2015-04-01 04:06:19.340124