pAAV SCH9
(Plasmid #212708)

• Purpose: A SCHEMA evolved AAV variant effective at targeting subventricular NSCs
• Depositing Lab: David Schaffer
• Publication: Ojala et al Mol Ther. 2018 Jan 3;26(1):304-319. doi: 10.1016/j.ymthe.2017.09.006. Epub 2017 Sep 8.

Backbone

• Vector backbone: pSub2Cap
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 8800
• Modifications to backbone: Removal of AAV ITR
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AAV2 Rep SCH9 Cap
• Species: AAV
• Insert Size (bp): 4800

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: GGTACGAAGCTTCGATCAACTACGCAG
• 3′ sequencing primer: AGCTAGCCTATTTACCGATAC

Resource Information

• Supplemental Documents:
  • sch9-2211-bp.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry